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Invitrogen
CD88 (C5aR1) Monoclonal Antibody (20/70), PE, eBioscience™
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 2
CD88 (C5aR1) Antibody (12-0882-82) in Flow
Mouse bone marrow cells were stained with CD11b Monoclonal Antibody, PE-Cyanine7 (Product # 25-0112-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, PE (Product # 12-4031-83) (left) or 0.25 µg of CD88 (C5aR1) Monoclonal Antibody, PE (right). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).
Product Details
12-0882-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
20/70
Immunogen
RBL-2H3 transfected cells expressing murine C5aR
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2811743
Product Specific Information
Description: This 20/70 monoclonal antibody recognizes mouse CD88 also known as C5aR1. CD88 is a complement receptor widely expressed on cells of the myeloid lineage. Clone 20/70 has been reported to block the binding of C5a to murine CD88.
Applications Reported: This 20/70 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 20/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
Target Information
CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a anaphylatoxin receptor; C5a ligand; C5a Receptor; C5a-R; CD88; CD88 antigen; complement component 5, receptor 1
Gene Aliases: C5aR; C5ar1; C5r1; Cd88; D7Msu1
UniProt ID: (Mouse) P30993
Entrez Gene ID: (Mouse) 12273
complement receptor mediated signaling pathway
apoptotic process
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
positive regulation of vascular endothelial growth factor production
positive regulation of macrophage chemotaxis
cell proliferation in hindbrain
neutrophil chemotaxis
response to peptidoglycan
complement component C5a signaling pathway
mRNA transcription from RNA polymerase II promoter
negative regulation of neuron apoptotic process
positive regulation of angiogenesis
positive regulation of epithelial cell proliferation
defense response to Gram-positive bacterium
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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