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CENPA Antibody (MA5-45299) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human colon cancer cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with CENP-A monoclonal antibody (Product # MA5-45299) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was DAPI (blue) nuclear stain at 1:5,000 for 5 min RT. Localization: Nucleus. Magnification: 60X.
Product Details
MA5-45299
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5A7-2E11
Immunogen
Synthetic peptide corresponding to a portion of human CENP-A
Conjugate
Excitation/Emission Max
482/675 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
47.35mM phosphate/2.15mM MES with 2mM EDTA, 0.17066µM Pentachlorophenol, 0.8mM ethanol
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931753
Product Specific Information
1 µg/mL of MA5-45299 was sufficient for detection of CENPA in 20 µg of U2OS cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 18kDa. Recognizes Human CENP-A.
Target Information
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CENP A; CENP-A; Centromere autoantigen A; Centromere protein A; centromere protein A, 17kDa; centromere-specific histone; Histone H3-like centromeric protein A
Gene Aliases: CenH3; CENP-A; CENPA
UniProt ID: (Human) P49450
Entrez Gene ID: (Human) 1058
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