Proteintech

CHMP2A Polyclonal Antibody

CHMP2A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CHMP2A Antibody (10477-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 10477-1-AP (CHMP2A antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Product Image Gallery

Product Details

10477-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 22 publications 22 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

View 18 publications 18 publications

Not applicable (N/A)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CHMP2A Fusion Protein Ag0719 (1-220 aa encoded by BC002502)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.19 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MDLLFGRRK TPEELLRQNQ RALNRAMREL DRERQKLETQ EKKIIADIKK MAKQGQMDAV RIMAKDLVRT RRYVRKFVLM RANIQAVSLK IQTLKSNNSM AQAMKGVTKA MGTMNRQLKL PQIQKIMMEF ERQAEIMDMK EEMMNDAIDD AMGDEEDEEE SDAVVSQVLD ELGLSLTDEL SNLPSTGGSL SVAAGGKKAE AAASALADAD ADLEERLKNL R (1-220 aa encoded by BC002502)

Target Information

CHMP2A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A is required for both MVB formation and regulation of cell cycle progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

An ATG12-ATG5-TECPR1 E3-like complex regulates unconventional LC3 lipidation at damaged lysosomes.

EMBO reports

Corkery DP,Castro-Gonzalez S,Knyazeva A,Herzog LK,Wu YW

Wed Sep 06 00:00:00 EDT 2023

Mammalian ATG8 proteins maintain autophagosomal membrane integrity through ESCRTs.

The EMBO journal

Javed R,Jain A,Duque T,Hendrix E,Paddar MA,Khan S,Claude-Taupin A,Jia J,Allers L,Wang F,Mudd M,Timmins G,Lidke K,Rusten TE,Akepati PR,He Y,Reggiori F,Eskelinen EL,Deretic V

Mon Jul 17 00:00:00 EDT 2023

Baicalein Suppresses NLRP3 and AIM2 Inflammasome-Mediated Pyroptosis in Macrophages Infected by Mycobacterium tuberculosis via Induced Autophagy.

Microbiology spectrum

Ning B,Shen J,Liu F,Zhang H,Jiang X

Thu Jun 15 00:00:00 EDT 2023

ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.

Developmental cell

Wang F,Peters R,Jia J,Mudd M,Salemi M,Allers L,Javed R,Duque TLA,Paddar MA,Trosdal ES,Phinney B,Deretic V

Mon May 22 00:00:00 EDT 2023

ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.

Developmental cell

Wang F,Peters R,Jia J,Mudd M,Salemi M,Allers L,Javed R,Duque TLA,Paddar MA,Trosdal ES,Phinney B,Deretic V

Mon May 22 00:00:00 EDT 2023

ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.

Developmental cell

Wang F,Peters R,Jia J,Mudd M,Salemi M,Allers L,Javed R,Duque TLA,Paddar MA,Trosdal ES,Phinney B,Deretic V

Mon May 22 00:00:00 EDT 2023

ATG5 provides host protection acting as a switch in the atg8ylation cascade between autophagy and secretion.

Developmental cell

Wang F,Peters R,Jia J,Mudd M,Salemi M,Allers L,Javed R,Duque TLA,Paddar MA,Trosdal ES,Phinney B,Deretic V

Mon May 22 00:00:00 EDT 2023

Downregulation of YAP Activity Restricts P53 Hyperactivation to Promote Cell Survival in Confinement.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Hemmati F,Akinpelu A,Song J,Amiri F,McDaniel A,McMurray C,Afthinos A,Andreadis ST,Aitken AV,Biancardi VC,Gerecht S,Mistriotis P

Tue Aug 01 00:00:00 EDT 2023

CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity.

Nature communications

Bernareggi D,Xie Q,Prager BC,Yun J,Cruz LS,Pham TV,Kim W,Lee X,Coffey M,Zalfa C,Azmoon P,Zhu H,Tamayo P,Rich JN,Kaufman DS

Thu Apr 07 00:00:00 EDT 2022

Polymyxin B Reduces Brain Injury in Ischemic Stroke Rat Through a Mechanism Involving Targeting ESCRT-III Machinery and RIPK1/RIPK3/MLKL Pathway.

Journal of cardiovascular translational research

Tian J,Zhang YY,Peng YW,Liu B,Zhang XJ,Hu ZY,Hu CP,Luo XJ,Peng J

Sat Oct 01 00:00:00 EDT 2022

V. cholerae MakA is a cholesterol-binding pore-forming toxin that induces non-canonical autophagy.

The Journal of cell biology

Jia X,Knyazeva A,Zhang Y,Castro-Gonzalez S,Nakamura S,Carlson LA,Yoshimori T,Corkery DP,Wu YW

Mon Dec 05 00:00:00 EST 2022

ATG9A protects the plasma membrane from programmed and incidental permeabilization.

Nature cell biology

Claude-Taupin A,Jia J,Bhujabal Z,Garfa-Traoré M,Kumar S,da Silva GPD,Javed R,Gu Y,Allers L,Peters R,Wang F,da Costa LJ,Pallikkuth S,Lidke KA,Mauthe M,Verlhac P,Uchiyama Y,Salemi M,Phinney B,Tooze SA,Mari MC,Johansen T,Reggiori F,Deretic V

Sun Aug 01 00:00:00 EDT 2021

An mTORC1-GRASP55 signaling axis controls unconventional secretion to reshape the extracellular proteome upon stress.

Molecular cell

Nüchel J,Tauber M,Nolte JL,Mörgelin M,Türk C,Eckes B,Demetriades C,Plomann M

Thu Aug 19 00:00:00 EDT 2021

Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes.

Cell

Kumar S,Javed R,Mudd M,Pallikkuth S,Lidke KA,Jain A,Tangavelou K,Gudmundsson SR,Ye C,Rusten TE,Anonsen JH,Lystad AH,Claude-Taupin A,Simonsen A,Salemi M,Phinney B,Li J,Guo LW,Bradfute SB,Timmins GS,Eskelinen EL,Deretic V

Wed Nov 24 00:00:00 EST 2021

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

PLoS genetics

Merigliano C,Burla R,La Torre M,Del Giudice S,Teo H,Liew CW,Chojnowski A,Goh WI,Olmos Y,Maccaroni K,Giubettini M,Chiolo I,Carlton JG,Raimondo D,Vernì F,Stewart CL,Rhodes D,Wright GD,Burke BE,Saggio I

Sun Aug 01 00:00:00 EDT 2021

Loss of Coiled-Coil Protein Cep55 Impairs Neural Stem Cell Abscission and Results in p53-Dependent Apoptosis in Developing Cortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Little JN,McNeely KC,Michel N,Bott CJ,Lettieri KS,Hecht MR,Martin SA,Dwyer ND

Wed Apr 14 00:00:00 EDT 2021

Targeting the ESCRT-III component CHMP2A for noncanonical Caspase-8 activation on autophagosomal membranes.

Cell death and differentiation

Hattori T,Takahashi Y,Chen L,Tang Z,Wills CA,Liang X,Wang HG

Mon Feb 01 00:00:00 EST 2021

PTEN alleviates maladaptive repair of renal tubular epithelial cells by restoring CHMP2A-mediated phagosome closure.

Cell death & disease

Wang H,Wang Y,Wang X,Huang H,Bao J,Zhong W,Li A

Tue Nov 16 00:00:00 EST 2021

The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model.

Nature communications

Yuste-Checa P,Trinkaus VA,Riera-Tur I,Imamoglu R,Schaller TF,Wang H,Dudanova I,Hipp MS,Bracher A,Hartl FU

Wed Aug 11 00:00:00 EDT 2021

CDK1 controls CHMP7-dependent nuclear envelope reformation.

eLife

Gatta AT,Olmos Y,Stoten CL,Chen Q,Rosenthal PB,Carlton JG

Wed Jul 21 00:00:00 EDT 2021

ER-directed TREX1 limits cGAS activation at micronuclei.

Molecular cell

Mohr L,Toufektchan E,von Morgen P,Chu K,Kapoor A,Maciejowski J

Thu Feb 18 00:00:00 EST 2021

The balance between AIM2-associated inflammation and autophagy: the role of CHMP2A in brain injury after cardiac arrest.

Journal of neuroinflammation

Shao R,Wang X,Xu T,Xia Y,Cui D

Fri Nov 05 00:00:00 EDT 2021

The balance between AIM2-associated inflammation and autophagy: the role of CHMP2A in brain injury after cardiac arrest.

Journal of neuroinflammation

Shao R,Wang X,Xu T,Xia Y,Cui D

Fri Nov 05 00:00:00 EDT 2021

Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.

Nature cell biology

Mercier V,Larios J,Molinard G,Goujon A,Matile S,Gruenberg J,Roux A

Sat Aug 01 00:00:00 EDT 2020

A non-canonical role for the autophagy machinery in anti-retroviral signaling mediated by TRIM5α.

PLoS pathogens

Saha B,Chisholm D,Kell AM,Mandell MA

Thu Oct 01 00:00:00 EDT 2020

Protein crowding mediates membrane remodeling in upstream ESCRT-induced formation of intraluminal vesicles.

Proceedings of the National Academy of Sciences of the United States of America

Liese S,Wenzel EM,Kjos I,Rojas Molina R,Schultz SW,Brech A,Stenmark H,Raiborg C,Carlson A

Tue Nov 17 00:00:00 EST 2020

ESCRT-mediated phagophore sealing during mitophagy.

Autophagy

Zhen Y,Spangenberg H,Munson MJ,Brech A,Schink KO,Tan KW,Sørensen V,Wenzel EM,Radulovic M,Engedal N,Simonsen A,Raiborg C,Stenmark H

Fri May 01 00:00:00 EDT 2020

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.

Nature cell biology

Vietri M,Schultz SW,Bellanger A,Jones CM,Petersen LI,Raiborg C,Skarpen E,Pedurupillay CRJ,Kjos I,Kip E,Timmer R,Jain A,Collas P,Knorr RL,Grellscheid SN,Kusumaatmaja H,Brech A,Micci F,Stenmark H,Campsteijn C

Wed Jul 01 00:00:00 EDT 2020

Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.

Nature cell biology

Wed Jul 01 00:00:00 EDT 2020

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Methods in molecular biology (Clifton, N.J.)

Adar-Levor S,Goliand I,Elbaum M,Elia N

Mon Mar 02 00:00:00 EST 2020

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 EDT 2018

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Nature

Liu S,Kwon M,Mannino M,Yang N,Renda F,Khodjakov A,Pellman D

Sat Sep 01 00:00:00 EDT 2018

Nonenvelopment Role for the ESCRT-III Complex during Human Cytomegalovirus Infection.

Journal of virology

Streck NT,Carmichael J,Buchkovich NJ

Fri Jun 15 00:00:00 EDT 2018

Nonenvelopment Role for the ESCRT-III Complex during Human Cytomegalovirus Infection.

Journal of virology

Streck NT,Carmichael J,Buchkovich NJ

Fri Jun 15 00:00:00 EDT 2018

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Scientific reports

Ott C,Nachmias D,Adar S,Jarnik M,Sherman S,Birnbaum RY,Lippincott-Schwartz J,Elia N

Tue Feb 20 00:00:00 EST 2018

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Scientific reports

Ott C,Nachmias D,Adar S,Jarnik M,Sherman S,Birnbaum RY,Lippincott-Schwartz J,Elia N

Tue Feb 20 00:00:00 EST 2018

CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.

Developmental cell

Ventimiglia LN,Cuesta-Geijo MA,Martinelli N,Caballe A,Macheboeuf P,Miguet N,Parnham IM,Olmos Y,Carlton JG,Weissenhorn W,Martin-Serrano J

Mon Dec 03 00:00:00 EST 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 EDT 2018

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Cell reports

Goliand I,Adar-Levor S,Segal I,Nachmias D,Dadosh T,Kozlov MM,Elia N

Tue Aug 14 00:00:00 EDT 2018

ESCRT-mediated lysosome repair precedes lysophagy and promotes cell survival.

The EMBO journal

Radulovic M,Schink KO,Wenzel EM,Nähse V,Bongiovanni A,Lafont F,Stenmark H

Fri Nov 02 00:00:00 EDT 2018

An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure.

Nature communications

Takahashi Y,He H,Tang Z,Hattori T,Liu Y,Young MM,Serfass JM,Chen L,Gebru M,Chen C,Wills CA,Atkinson JM,Chen H,Abraham T,Wang HG

Fri Jul 20 00:00:00 EDT 2018

An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure.

Nature communications

Takahashi Y,He H,Tang Z,Hattori T,Liu Y,Young MM,Serfass JM,Chen L,Gebru M,Chen C,Wills CA,Atkinson JM,Chen H,Abraham T,Wang HG

Fri Jul 20 00:00:00 EDT 2018

Nuclear Envelope Rupture Is Enhanced by Loss of p53 or Rb.

Molecular cancer research : MCR

Yang Z,Maciejowski J,de Lange T

Wed Nov 01 00:00:00 EDT 2017

Nuclear Envelope Rupture Is Enhanced by Loss of p53 or Rb.

Molecular cancer research : MCR

Yang Z,Maciejowski J,de Lange T

Wed Nov 01 00:00:00 EDT 2017

Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.

Current biology : CB

Olmos Y,Perdrix-Rosell A,Carlton JG

Mon Oct 10 00:00:00 EDT 2016

Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.

Current biology : CB

Olmos Y,Perdrix-Rosell A,Carlton JG

Mon Oct 10 00:00:00 EDT 2016

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 EDT 2015

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 EDT 2015

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 EDT 2015

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Nature

Vietri M,Schink KO,Campsteijn C,Wegner CS,Schultz SW,Christ L,Thoresen SB,Brech A,Raiborg C,Stenmark H

Thu Jun 11 00:00:00 EDT 2015

ESCRT-III controls nuclear envelope reformation.

Nature

Olmos Y,Hodgson L,Mantell J,Verkade P,Carlton JG

Thu Jun 11 00:00:00 EDT 2015

ESCRT-III controls nuclear envelope reformation.

Nature

Olmos Y,Hodgson L,Mantell J,Verkade P,Carlton JG

Thu Jun 11 00:00:00 EDT 2015

ESCRT-III controls nuclear envelope reformation.

Nature

Olmos Y,Hodgson L,Mantell J,Verkade P,Carlton JG

Thu Jun 11 00:00:00 EDT 2015

ESCRT-III controls nuclear envelope reformation.

Nature

Olmos Y,Hodgson L,Mantell J,Verkade P,Carlton JG

Thu Jun 11 00:00:00 EDT 2015

MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis.

Molecular biology of the cell

Lee S,Chang J,Renvoisé B,Tipirneni A,Yang S,Blackstone C

Thu Nov 01 00:00:00 EDT 2012

MITD1 is recruited to midbodies by ESCRT-III and participates in cytokinesis.

Molecular biology of the cell

Lee S,Chang J,Renvoisé B,Tipirneni A,Yang S,Blackstone C

Thu Nov 01 00:00:00 EDT 2012

Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15.

Journal of virology

Kuang Z,Seo EJ,Leis J

Fri Jul 01 00:00:00 EDT 2011

Mechanism of inhibition of retrovirus release from cells by interferon-induced gene ISG15.

Journal of virology

Kuang Z,Seo EJ,Leis J

Fri Jul 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: Charged multivesicular body protein 2a; CHMP2a; chromatin modifying protein 2A; Chromatin-modifying protein 2a; mVps2; putative breast adenocarcinoma marker (32kD); Putative breast adenocarcinoma marker BC-2; Vacuolar protein sorting-associated protein 2; Vacuolar protein sorting-associated protein 2-1; vacuolar protein-sorting-associated protein 2; VPS2 homolog A; Vps2-1

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Gene Aliases: 1500016L11Rik; BC-2; BC2; CHMP2; CHMP2A; RGD1305050; VPS2; VPS2A

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UniProt ID: (Human) O43633, (Mouse) Q9DB34

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Entrez Gene ID: (Human) 27243, (Rat) 365191, (Mouse) 68953

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Function(s)

protein binding protein domain specific binding phosphatidylcholine binding membrane traffic protein

Process(es)

cell separation after cytokinesis autophagy nucleus organization mitotic metaphase plate congression membrane invagination exit from mitosis regulation of centrosome duplication protein transport endosomal transport viral life cycle nuclear envelope reassembly multivesicular body assembly viral budding via host ESCRT complex establishment of protein localization regulation of viral process protein polymerization protein homooligomerization protein heterooligomerization negative regulation of cell death regulation of mitotic spindle assembly positive regulation of viral release from host cell positive regulation of exosomal secretion negative regulation of centriole elongation ESCRT III complex disassembly vacuolar transport transport

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CHMP2A Polyclonal Antibody

CHMP2A Antibody (10477-1-AP) in IHC (P)

Product Details

10477-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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