Invitrogen

CXCR4 Monoclonal Antibody (12G5)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

10 Published Figures

29 References

View all (76) CXCR4 (CD184) antibodies

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CXCR4 Antibody (35-8800) in Flow

Knockout of CXCR4 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR695599_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CXCR4 was performed by staining HeLa CXCR4 Knock out cells with4 µg Mouse / IgG2a, kappa (Product # 14-4724-82, yellow histogram) or 4 µg CXCR4 Monoclonal Antibody (12G5) (Product # 358800, blue histogram) followed by Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A55... View More

CXCR4 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

CXCR4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CXCR4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CXCR4 Antibody — The specificity of anti-CXCR4 antibody (Product # 35-8800) was demonstrated by the western blot detection of specific expression of CXCR2 in Ramos and Raji cells, but not in negative control HepG2 cells. NOTE: The presence of a 60kD band likely represents an isoform or post-translationally modified form of CXCR4, many of which have been described in the literature. {RE}

Product Details

35-8800

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

Assay-dependent

View 4 publications 4 publications

Flow Cytometry (Flow)

3-5 µg/mL

View 6 publications 6 publications

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

12G5

Immunogen

SIV-infected SupT1 cells injected into Balb/c mice.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533227

Product Specific Information

By Western blot, 35-8800 detects a band at ~60kD, which likely represents an isoform or post-translationally modified form of CXCR4, many of which have been described in the literature.

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nanoparticle elasticity regulates the formation of cell membrane-coated nanoparticles and their nano-bio interactions.

Proceedings of the National Academy of Sciences of the United States of America

Zou D,Wu Z,Yi X,Hui Y,Yang G,Liu Y,Wang H,Brooks A,Wang H,Liu X,Xu ZP,Roberts MS,Gao H,Zhao CX

Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

β1-Integrin plays a major role in resveratrol-mediated anti-invasion effects in the CRC microenvironment.

Frontiers in pharmacology

Brockmueller A,Mueller AL,Shayan P,Shakibaei M

Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Western Blot

Tue Sep 20 00:00:00 EDT 2022

EGFR Activates a TAZ-Driven Oncogenic Program in Glioblastoma.

Cancer research

Gao M,Fu Y,Zhou W,Gui G,Lal B,Li Y,Xia S,Ji H,Eberhart CG,Laterra J,Ying M

35-8800 was used in Immunocytochemistry to show that the therapeutic efficacy of osimertinib relies on effective TAZ inhibition, thus identifying TAZ as a potential biomarker of osimertinib sensitivity.

Thu Jul 01 00:00:00 EDT 2021

CXCR4 promotes B cell viability by the cooperation of nuclear factor (erythroid-derived 2)-like 2 and hypoxia-inducible factor-1α under hypoxic conditions.

Cell death & disease

Jang JW,Thuy PX,Lee JW,Moon EY

Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Western Blot

Fri Mar 26 00:00:00 EDT 2021

MiR-140-3p Ameliorates the Progression of Osteoarthritis via Targeting CXCR4.

Biological & pharmaceutical bulletin

Ren T,Wei P,Song Q,Ye Z,Wang Y,Huang L

35-8800 was used in Western Blotting to evaluate the potential function of miRNA-140-3p during the pathogenesis of osteoarthritis.

Fri May 01 00:00:00 EDT 2020

Optimized nonviral gene delivery for primary urinary renal progenitor cells to enhance cell migration.

Journal of biomedical materials research. Part A

Liu GW,Johnson SL,Jain R,Peeler DJ,Shankland SJ,Pun SH

35-8800 was used in Flow Cytometry to transfect uRPCs to overexpress CXCR4, an important chemokine receptor that mediates uRPC migration to the kidneys after intravenous injection, and demonstrate that CXCR4-uRPCs exhibit enhanced migration compared to mock-transfected cells.

Sun Dec 01 00:00:00 EST 2019

Cocktail Strategy Based on NK Cell-Derived Exosomes and Their Biomimetic Nanoparticles for Dual Tumor Therapy.

Cancers

Wang G,Hu W,Chen H,Shou X,Ye T,Xu Y

35-8800 was used in Western Blotting to propose this NN/NKEXO cocktail as a new avenue for tumor therapy, with potential prospects for clinical applications.

Mon Oct 14 00:00:00 EDT 2019

The Role of C-X-C Chemokine Receptor Type 4 (CXCR4) in Cell Adherence and Spheroid Formation of Human Ewing's Sarcoma Cells under Simulated Microgravity.

International journal of molecular sciences

Romswinkel A,Infanger M,Dietz C,Strube F,Kraus A

35-8800 was used in Western Blotting to studied the behavior of Ewing's Sarcoma cells of the line A673 under simulated microgravity.

Mon Dec 02 00:00:00 EST 2019

PLCε1 regulates SDF-1α-induced lymphocyte adhesion and migration to sites of inflammation.

Proceedings of the National Academy of Sciences of the United States of America

Strazza M,Azoulay-Alfaguter I,Peled M,Smrcka AV,Skolnik EY,Srivastava S,Mor A

358800 was used in flow cytometry to discover new regulatory proteins involved in Rap1-dependent T-cell adhesion and migration.

Tue Mar 07 00:00:00 EST 2017

In vitro formation of neuroclusters in microfluidic devices and cell migration as a function of stromal-derived growth factor 1 gradients.

Cell adhesion & migration

McCutcheon S,Unachukwu U,Thakur A,Majeska R,Redenti S,Vazquez M

Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Immunocytochemistry

Mon Jan 02 00:00:00 EST 2017

In vitro formation of neuroclusters in microfluidic devices and cell migration as a function of stromal-derived growth factor 1 gradients.

Cell adhesion & migration

McCutcheon S,Unachukwu U,Thakur A,Majeska R,Redenti S,Vazquez M

Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Immunocytochemistry

Mon Jan 02 00:00:00 EST 2017

In vitro formation of neuroclusters in microfluidic devices and cell migration as a function of stromal-derived growth factor 1 gradients.

Cell adhesion & migration

McCutcheon S,Unachukwu U,Thakur A,Majeska R,Redenti S,Vazquez M

Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Immunocytochemistry

Mon Jan 02 00:00:00 EST 2017

Distinct bone marrow blood vessels differentially regulate haematopoiesis.

Nature

Itkin T,Gur-Cohen S,Spencer JA,Schajnovitz A,Ramasamy SK,Kusumbe AP,Ledergor G,Jung Y,Milo I,Poulos MG,Kalinkovich A,Ludin A,Kollet O,Shakhar G,Butler JM,Rafii S,Adams RH,Scadden DT,Lin CP,Lapidot T

35-8800 was used in Functional assay to show that these findings may have relevance for clinical haematopoietic stem cell transplantation and mobilization protocols.

Thu Apr 21 00:00:00 EDT 2016

Circulating Progenitor Cells is Linked to Cognitive Decline in Healthy Adults.

The American journal of the medical sciences

Hajjar I,Goldstein FC,Waller EK,Moss LD,Quyyumi A

358800 was used in flow cytometry to investigate the association between progenitor cells and cognition in a sample of healthy adults

Mon Feb 01 00:00:00 EST 2016

Pantethine Alters Lipid Composition and Cholesterol Content of Membrane Rafts, With Down-Regulation of CXCL12-Induced T Cell Migration.

Journal of cellular physiology

van Gijsel-Bonnello M,Acar N,Molino Y,Bretillon L,Khrestchatisky M,de Reggi M,Gharib B

35-8800 was used in immunocytochemistry to examine the effects of pantethine on membrane lipid rafts.

Thu Oct 01 00:00:00 EDT 2015

EGF as a New Therapeutic Target for Medulloblastoma Metastasis.

Cellular and molecular bioengineering

Rico-Varela J,Singh T,McCutcheon S,Vazquez M

35-8800 was used in immunocytochemistry to examine the role of epidermal growth factor in medulloblastoma chemotactic migration

Tue Dec 01 00:00:00 EST 2015

Immunohistochemical Predictors of Bone Metastases in Breast Cancer Patients.

Pathology oncology research : POR

Winczura P,Sosińska-Mielcarek K,Duchnowska R,Badzio A,Lakomy J,Majewska H,Pęksa R,Pieczyńska B,Radecka B,Dębska-Szmich S,Adamowicz K,Biernat W,Jassem J

35-8800 was used in immunohistochemistry to evaluate the potential protein markers of bone metastases in breast cancer patients.

Tue Sep 01 00:00:00 EDT 2015

Brain metastasis in renal cancer patients: metastatic pattern, tumour-associated macrophages and chemokine/chemoreceptor expression.

British journal of cancer

Wyler L,Napoli CU,Ingold B,Sulser T,Heikenwälder M,Schraml P,Moch H

35-8800 was used in immunohistochemistry - paraffin section to discuss the role of chemokines in brain metastasis observed in patients with renal cell cancer

Tue Feb 04 00:00:00 EST 2014

Immunohistochemical analysis of cancer stem cell markers in pancreatic adenocarcinoma patients after neoadjuvant chemoradiotherapy.

BMC cancer

Mizukami T,Kamachi H,Mitsuhashi T,Tsuruga Y,Hatanaka Y,Kamiyama T,Matsuno Y,Taketomi A

35-8800 was used in immunohistochemistry - paraffin section to investigate the effect of neoadjuvant chemoradiotherapy on pancreatic adenocarcinoma cancer stem cell markers

Sun Sep 21 00:00:00 EDT 2014

Immunohistochemical prediction of brain metastases in patients with advanced breast cancer: the role of Rad51.

Breast (Edinburgh, Scotland)

Sosińska-Mielcarek K,Duchnowska R,Winczura P,Badzio A,Majewska H,Lakomy J,Pęksa R,Pieczyńska B,Radecka B,Dębska S,Biernat W,Jassem J

35-8800 was used in immunohistochemistry to correlate expression of proteins in the primary tumor with the risk of brain metastases in patients with metastatic breast cancer.

Sun Dec 01 00:00:00 EST 2013

Participation of peribiliary glands in biliary tract pathophysiologies.

World journal of hepatology

Igarashi S,Sato Y,Ren XS,Harada K,Sasaki M,Nakanuma Y

Published figure using CXCR4 monoclonal antibody (Product # 35-8800) in Immunohistochemistry

Tue Aug 27 00:00:00 EDT 2013

Hypoxia induces CXCR4 expression and biological activity in gastric cancer cells through activation of hypoxia-inducible factor-1α.

Oncology reports

Oh YS,Kim HY,Song IC,Yun HJ,Jo DY,Kim S,Lee HJ

35-8800 was used in Western Blotting to study the role of hypoxia in regulating CXCR4 expression and biological activity in gastric cancer.

Sat Dec 01 00:00:00 EST 2012

Co-culture of hematopoietic stem cells with mesenchymal stem cells increases VCAM-1-dependent migration of primitive hematopoietic stem cells.

International journal of hematology

Perdomo-Arciniegas AM,Vernot JP

35-8800 was used in flow cytometry to investigate how mesenchymal stem cells improve hematopoietic stem cell transplantability.

Thu Dec 01 00:00:00 EST 2011

Increased levels of active c-Src distinguish invasive from in situ lobular lesions.

Breast cancer research : BCR

Zou D,Yoon HS,Anjomshoaa A,Perez D,Fukuzawa R,Guilford P,Humar B

Wed Feb 24 00:00:00 EST 2010

Comparative analysis of tumorbiology and CD133 positivity in primary and recurrent pancreatic ductal adenocarcinoma.

Clinical & experimental metastasis

Welsch T,Keleg S,Bergmann F,Degrate L,Bauer S,Schmidt J

35-8800 was used in immunohistochemistry - paraffin section to measure the expression of CXCR4, actinin-4, and CD133 in primary and recurrent pancreatic ductal adenocarcinoma

Fri Nov 27 00:00:00 EST 2009

GM-CSF DNA: an adjuvant for higher avidity IgG, rectal IgA, and increased protection against the acute phase of a SHIV-89.6P challenge by a DNA/MVA immunodeficiency virus vaccine.

Virology

Lai L,Vödrös D,Kozlowski PA,Montefiori DC,Wilson RL,Akerstrom VL,Chennareddi L,Yu T,Kannanganat S,Ofielu L,Villinger F,Wyatt LS,Moss B,Amara RR,Robinson HL

358800 was used in flow cytometry to assess the effects of using GM-CSF DNA with the DNA prime for a simian-human immunodeficiency virus-89.6 vaccine

Wed Dec 05 00:00:00 EST 2007

Gene expression profiling identifies genes associated with invasive intraductal papillary mucinous neoplasms of the pancreas.

The American journal of pathology

Sato N,Fukushima N,Maitra A,Iacobuzio-Donahue CA,van Heek NT,Cameron JL,Yeo CJ,Hruban RH,Goggins M

35-8800 was used in immunohistochemistry - paraffin section to examine the gene expression profiles of intraductal papillary mucinous neoplasms

Mon Mar 01 00:00:00 EST 2004

Acquisition of lymph node, but not distant metastatic potentials, by the overexpression of CXCR4 in human oral squamous cell carcinoma.

Laboratory investigation; a journal of technical methods and pathology

Uchida D,Begum NM,Tomizuka Y,Bando T,Almofti A,Yoshida H,Sato M

35-8800 was used in Immunohistochemistry to indicate that SDF-1/CXCR4 signaling mediates the establishment of lymph node metastasis in oral SCC via ERK1/2 or Akt/PKB pathway.

Wed Dec 01 00:00:00 EST 2004

CD4-independent infection by HIV-2 is mediated by fusin/CXCR4.

Cell

Endres MJ,Clapham PR,Marsh M,Ahuja M,Turner JD,McKnight A,Thomas JF,Stoebenau-Haggarty B,Choe S,Vance PJ,Wells TN,Power CA,Sutterwala SS,Doms RW,Landau NR,Hoxie JA

Fri Nov 15 00:00:00 EST 1996

CD4-independent infection by HIV-2 is mediated by fusin/CXCR4.

Cell

Endres MJ,Clapham PR,Marsh M,Ahuja M,Turner JD,McKnight A,Thomas JF,Stoebenau-Haggarty B,Choe S,Vance PJ,Wells TN,Power CA,Sutterwala SS,Doms RW,Landau NR,Hoxie JA

Fri Nov 15 00:00:00 EST 1996

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine receptor; CXC; CXC motif chemokine; CXC-R4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

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Gene Aliases: CD184; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; WHIM; WHIMS

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UniProt ID: (Human) P61073

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Entrez Gene ID: (Human) 7852

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Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding

Process(es)

activation of MAPK activity response to hypoxia neuron migration epithelial cell development dendritic cell chemotaxis apoptotic process inflammatory response G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration neuron recognition response to virus fusion of virus membrane with host plasma membrane calcium-mediated signaling telencephalon cell migration entry into host cell regulation of cell migration regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus

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CXCR4 Monoclonal Antibody (12G5)

CXCR4 Antibody (35-8800) in Flow

Product Details

35-8800

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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