Invitrogen

Calprotectin Monoclonal Antibody (MAC387)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4 Published Figures

25 References

View all (98) Calprotectin antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Calprotectin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 19

Calprotectin Antibody (MA1-81381) in ICC/IF

Immunofluorescence analysis of Calprotectin (S100A9) was performed using 70% confluent log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-80155) at 1:250 dilution ... View More

Calprotectin Antibody in Western Blot (WB)

Calprotectin Antibody in Immunohistochemistry (IHC)

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Product Details

MA1-81381

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:200

Immunocytochemistry (ICC/IF)

1:250

- -

Flow Cytometry (Flow)

1:50-1:100

View 4 publications 4 publications

Product Specifications

Species Reactivity

Goat, Human, Non-human primate, Pig, Rabbit, Rat, Bovine, Dog, Deer, Horse, Cat, Guinea pig

Published species

Bovine, Cynomolgus monkey, Dog, Horse, Human, Mouse, Pig, Rat, Rhesus monkey

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

MAC387

Immunogen

Human monocytes.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2184262

Product Specific Information

This clone recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha. The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species.

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase prior to IHC staining. A suggested positive control is human spleen. For flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Equine cervical remodeling during placentitis and the prepartum period: a transcriptomic approach.

Reproduction (Cambridge, England)

El-Sheikh Ali H,Scoggin KE,Ruby R,Loynachan A,Boakari Y,Fernandes C,Dini P,Fedorka CE,Loux SC,Esteller-Vico A,Ball BA

MA1-81381 was used in Immunohistochemistry to investigate the molecular mechanisms underlying cervical remodeling.

Wed May 05 00:00:00 EDT 2021

High Numbers of CD163-Positive Macrophages in the Fibrotic Region of Exuberant Granulation Tissue in Horses.

Animals : an open access journal from MDPI

Du Cheyne C,Martens A,De Spiegelaere W

Published figure using Calprotectin monoclonal antibody (Product # MA1-81381) in Immunohistochemistry

Sat Sep 18 00:00:00 EDT 2021

High Numbers of CD163-Positive Macrophages in the Fibrotic Region of Exuberant Granulation Tissue in Horses.

Animals : an open access journal from MDPI

Du Cheyne C,Martens A,De Spiegelaere W

Published figure using Calprotectin monoclonal antibody (Product # MA1-81381) in Immunohistochemistry

Sat Sep 18 00:00:00 EDT 2021

Reinforcement of Colonic Anastomosis with Improved Ultrafine Nanofibrous Patch: Experiment on Pig.

Biomedicines

Rosendorf J,Klicova M,Cervenkova L,Horakova J,Klapstova A,Hosek P,Palek R,Sevcik J,Polak R,Treska V,Chvojka J,Liska V

MA1-81381 was used in Immunohistochemistry to show that the levels of collagen were significantly higher in the Experimental group, which we consider to be an indirect sign of higher mechanical strength.

Thu Jan 21 00:00:00 EST 2021

Reinforcement of Colonic Anastomosis with Improved Ultrafine Nanofibrous Patch: Experiment on Pig.

Biomedicines

Rosendorf J,Klicova M,Cervenkova L,Horakova J,Klapstova A,Hosek P,Palek R,Sevcik J,Polak R,Treska V,Chvojka J,Liska V

Thu Jan 21 00:00:00 EST 2021

Experimental fortification of intestinal anastomoses with nanofibrous materials in a large animal model.

Scientific reports

Rosendorf J,Horakova J,Klicova M,Palek R,Cervenkova L,Kural T,Hosek P,Kriz T,Tegl V,Moulisova V,Tonar Z,Treska V,Lukas D,Liska V

Published figure using Calprotectin monoclonal antibody (Product # MA1-81381) in Immunohistochemistry

Fri Jan 24 00:00:00 EST 2020

Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration.

Xenotransplantation

Tee BC,Sun Z

MA1-81381 was used in Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) to examine whether the immune rejection of xenogeneic stem cell transplantation can be evaded by diminishing T-cell immunity using a rat critical-size mandibular defect model.

Tue Sep 01 00:00:00 EDT 2020

Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.

Mucosal immunology

Gideon HP,Phuah J,Junecko BA,Mattila JT

MA1-80155 was used in Immunohistochemistry, Flow Cytometry to compare neutrophil and T-cell expression of the Th1 cytokines IFNγ and TNF, the Th2 cytokine IL-4, and regulatory cytokine IL-10 in M. tuberculosis-infected macaques.

Fri Nov 01 00:00:00 EDT 2019

Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.

Mucosal immunology

Gideon HP,Phuah J,Junecko BA,Mattila JT

Fri Nov 01 00:00:00 EDT 2019

Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.

Mucosal immunology

Gideon HP,Phuah J,Junecko BA,Mattila JT

Fri Nov 01 00:00:00 EDT 2019

Neutrophils express pro- and anti-inflammatory cytokines in granulomas from Mycobacterium tuberculosis-infected cynomolgus macaques.

Mucosal immunology

Gideon HP,Phuah J,Junecko BA,Mattila JT

Fri Nov 01 00:00:00 EDT 2019

A Skin Rejection Grading System for Vascularized Composite Allotransplantation in a Preclinical Large Animal Model.

Transplantation

Etra JW,Grzelak MJ,Fidder SAJ,Kolegraff K,Bonawitz S,Shores J,Oh B,Cooney DS,Beck SE,Brandacher G

MA1-80446 was used in Immunohistochemistry to provide a standardized system for more accurate comparison of rejection in preclinical swine VCA models.

Mon Jul 01 00:00:00 EDT 2019

Circulating monocytes accelerate acute liver failure by IL-6 secretion in monkey.

Journal of cellular and molecular medicine

Guo G,Zhu Y,Wu Z,Ji H,Lu X,Zhou Y,Li Y,Cao X,Lu Y,Talbot P,Liao J,Shi Y,Bu H

MA1-80155 was used in Immunohistochemistry-immunofluorescence to identify the critical role of circulating monocytes in initiating and accelerating acute liver failure.

Sat Sep 01 00:00:00 EDT 2018

Differential Interstrain Susceptibility to Vertebrobasilar Dolichoectasia in a Mouse Model.

AJNR. American journal of neuroradiology

Zhu YQ,Xing H,Dai D,Kallmes DF,Kadirvel R

MA181381 was used in immunohistochemistry - paraffin section to assess the differential susceptibility to elastase-induced vertebrobasilar dolichoectasia induction

Wed Mar 01 00:00:00 EST 2017

Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Infection and immunity

Phuah J,Wong EA,Gideon HP,Maiello P,Coleman MT,Hendricks MR,Ruden R,Cirrincione LR,Chan J,Lin PL,Flynn JL

MA1-80155 was used in Flow cytometry/Cell sorting to examine the contribution of B cells and humoural immunity to the control of tuberculosis in nonhuman primates during the acute phase of infection.

Sun May 01 00:00:00 EDT 2016

Morphological and physiological retinal degeneration induced by intravenous delivery of vitamin A dimers in rabbits.

Disease models & mechanisms

Penn J,Mihai DM,Washington I

MA1-81381 was used in immunohistochemistry - paraffin section to determine the induction by intravenous delivery of vitamin A dimers in rabbits due to physiological and morphological retinal degeneration

Sun Feb 01 00:00:00 EST 2015

Polymeric embolization coil of bilayered polyvinyl alcohol strand for therapeutic vascular occlusion: a feasibility study in canine experimental vascular models.

Journal of vascular and interventional radiology : JVIR

Jung SC,Choi SH,Cho HR,Lee TH,Kim TY,Jeong W,Rhee K,Jho JY,Kim JH,Han MH

MA1-81381 was used in immunohistochemistry to test polyvinyl alcohol polymer coil as an endovascular embolic agent in a canine vascular model

Thu Jan 01 00:00:00 EST 2015

Identification of the critical therapeutic entity in secreted Hsp90α that promotes wound healing in newly re-standardized healthy and diabetic pig models.

PloS one

O'Brien K,Bhatia A,Tsen F,Chen M,Wong AK,Woodley DT,Li W

MA1-80446 was used in immunohistochemistry - paraffin section to characterize the therapeutic component of secreted Hsp90alpha that promotes wound healing in diabetic and healthy pig models that were re-standardized

Mon Aug 31 00:00:00 EDT 2015

Microenvironments in tuberculous granulomas are delineated by distinct populations of macrophage subsets and expression of nitric oxide synthase and arginase isoforms.

Journal of immunology (Baltimore, Md. : 1950)

Mattila JT,Ojo OO,Kepka-Lenhart D,Marino S,Kim JH,Eum SY,Via LE,Barry CE,Klein E,Kirschner DE,Morris SM,Lin PL,Flynn JL

MA1-80155 was used in Flow cytometry/Cell sorting to study macrophage diversity and function within the complex structures of granulomas.

Mon Jul 15 00:00:00 EDT 2013

Xenotransplantation of human unrestricted somatic stem cells in a pig model of acute myocardial infarction.

Xenotransplantation

Gahremanpour A,Vela D,Zheng Y,Silva GV,Fodor W,Cardoso CO,Baimbridge F,Fernandes MR,Buja LM,Perin EC

MA1-81381 was used in immunohistochemistry to study whether xenotransplantation with human CD34-/CD45- unrestricted somatic stem cells is of therapeutic benefit in a porcine model of acute myocardial infarction

Mon Sep 30 00:00:00 EDT 2013

Transplantation with autologous mesenchymal stem cells after acute myocardial infarction evaluated by magnetic resonance imaging: an experimental study.

Journal of thoracic imaging

Lu M,Zhao S,Liu Q,Jiang S,Song P,Qian H,Zhang Y,Ling J,Yan C,Cheng H,Ma N,Zhao H,Liu Y

MA1-81381 was used in immunohistochemistry to study the effects of transplantation with autologous mesenchymal stem cells in postinfarction swine hearts

Thu Mar 01 00:00:00 EST 2012

Gene expression profile of vascular endothelial growth factor (VEGF) and its receptors in various cell types of the canine lymph node using laser capture microdissection (LCM).

Veterinary immunology and immunopathology

Jais A,Klein D,Wolfesberger B,Walter I

MA1-81381 was used in immunohistochemistry to study the expression of VEGF and its receptors in a range of cell types in normal canine lymph nodes.

Fri Apr 15 00:00:00 EDT 2011

The performance of photooxidatively crosslinked acellular bovine jugular vein conduits in the reconstruction of connections between pulmonary arteries and right ventricles.

Biomaterials

Lü WD,Zhang M,Wu ZS,Hu TH,Xu ZJ,Liu W,Hu YR

MA1-81381 was used in immunohistochemistry to evaluate photooxidatively crosslinked acellular bovine jugular vein conduits in vascular reconstruction

Thu Apr 01 00:00:00 EDT 2010

Insulin increases resistance to burn wound infection-associated sepsis.

Critical care medicine

Gauglitz GG,Toliver-Kinsky TE,Williams FN,Song J,Cui W,Herndon DN,Jeschke MG

MA1-81381 was used in flow cytometry to investigate the therapeutic efficacy of insulin on the treatment of sepsis

Fri Jan 01 00:00:00 EST 2010

Chronic intake of a high-cholesterol diet resulted in hepatic steatosis, focal nodular hyperplasia and fibrosis in non-obese mice.

The British journal of nutrition

Sumiyoshi M,Sakanaka M,Kimura Y

MA1-81381 was used in immunohistochemistry to investigate the influence of high-cholesterol food on liver function in normal mice

Mon Feb 01 00:00:00 EST 2010

Characterization of a preclinical model of chronic ischemic wound.

Physiological genomics

Roy S,Biswas S,Khanna S,Gordillo G,Bergdall V,Green J,Marsh CB,Gould LJ,Sen CK

MA1-81381 was used in immunohistochemistry to develop and evaluate a preclinical porcine model of chronic ischemic wound

Wed May 13 00:00:00 EDT 2009

Role of cardiopulmonary bypass and arrested heart status in the early cell distribution after intracoronary infusion of bone marrow stromal cells.

The Journal of surgical research

Song P,Zhang H,Lu MJ,Li J,Liu XW,Wei YJ,Hu SS

MA1-81381 was used in immunohistochemistry to study the distribution of cells following intracoronary infusion of bone marrow stromal cells

Fri May 01 00:00:00 EDT 2009

Injection of bone marrow mesenchymal stem cells in the borderline area of infarcted myocardium: heart status and cell distribution.

The Journal of thoracic and cardiovascular surgery

Zhang H,Song P,Tang Y,Zhang XL,Zhao SH,Wei YJ,Hu SS

MA1-80381 was used in immunohistochemistry to study the distribution of bone marrow mesenchymal stem cells following injection into infarcted myocardium

Thu Nov 01 00:00:00 EDT 2007

Histopathology of spleen allograft rejection in miniature swine.

International journal of experimental pathology

Dor FJ,Gollackner B,Kuwaki K,Ko DS,Cooper DK,Houser SL

MA1-80446 was used in immunohistochemistry to study the histopathology of spleen allograft rejection in miniature swine

Tue Feb 01 00:00:00 EST 2005

Bioinformatics

Protein Aliases: AI323541; B8Ag; BEE11; BEE22; calcium binding protein; S100 protein; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; calprotectin; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; macrophage migration inhibitory factor-related protein-14; macrophage migration inhibitory factor-related protein-8; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; p14; p23; P7; p8; Protein S100-A8; Protein S100-A9; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8/S100A9; Urinary stone protein band A

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Gene Aliases: 60B8AG; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; L1AG; LIAG; MA387; MAC387; MIF; MRP-14; MRP-8; MRP14; MRP8; NIF; P14; P8; S100A8; S100A9

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UniProt ID: (Bovine) P28782, (Human) P05109, (Bovine) P28783, (Rabbit) P50117, (Human) P06702, (Rat) P50115, (Rat) P50116

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Function(s)

calcium ion binding microtubule binding zinc ion binding antioxidant activity Toll-like receptor 4 binding arachidonic acid binding RAGE receptor binding protein binding signal transducer activity molecular_function calmodulin-related

Process(es)

leukocyte migration involved in inflammatory response positive regulation of peptide secretion autophagy apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process astrocyte development peptidyl-cysteine S-nitrosylation neutrophil chemotaxis innate immune response positive regulation of inflammatory response neutrophil aggregation cellular oxidant detoxification positive regulation of intrinsic apoptotic signaling pathway cytokine production acute inflammatory response chronic inflammatory response inflammatory response response to zinc ion positive regulation of cell growth sequestering of zinc ion response to lipopolysaccharide chemokine production wound healing defense response to bacterium response to ethanol defense response to fungus positive regulation of NF-kappaB transcription factor activity regulation of cytoskeleton organization regulation of translation signal transduction cell-cell signaling positive regulation of blood coagulation actin cytoskeleton reorganization peptidyl-cysteine S-trans-nitrosylation regulation of integrin biosynthetic process leukocyte chemotaxis

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Calprotectin Monoclonal Antibody (MAC387)

Calprotectin Antibody (MA1-81381) in ICC/IF

Product Details

MA1-81381

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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