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Product Details
MA5-28129
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MAC387
Immunogen
Human monocytes
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
Protein A
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745112
Product Specific Information
Reconstitute with 1.0 mL distilled water
Mouse anti Human macrophages, clone MAC387 recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain.
Target Information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AI323541; B8Ag; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Protein S100-A8; Protein S100-A9; S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8/S100A9; Urinary stone protein band A
Gene Aliases: 60B8AG; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; L1AG; LIAG; MA387; MAC387; MIF; MRP14; MRP8; NIF; P14; P8; S100A8; S100A9
UniProt ID: (Human) P05109, (Human) P06702
Entrez Gene ID: (Human) 6279, (Human) 6280
cytokine production
leukocyte migration involved in inflammatory response
acute inflammatory response
chronic inflammatory response
positive regulation of peptide secretion
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
inflammatory response
response to zinc ion
astrocyte development
peptidyl-cysteine S-nitrosylation
positive regulation of cell growth
neutrophil chemotaxis
sequestering of zinc ion
response to lipopolysaccharide
chemokine production
wound healing
defense response to bacterium
innate immune response
response to ethanol
positive regulation of inflammatory response
defense response to fungus
positive regulation of NF-kappaB transcription factor activity
regulation of cytoskeleton organization
neutrophil aggregation
positive regulation of intrinsic apoptotic signaling pathway
regulation of translation
signal transduction
cell-cell signaling
positive regulation of blood coagulation
actin cytoskeleton reorganization
peptidyl-cysteine S-trans-nitrosylation
regulation of integrin biosynthetic process
cellular oxidant detoxification
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