Invitrogen

EGR2 Monoclonal Antibody (erongr2), PE, eBioscience™

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Antibody Testing Data (1)
Published Figures (11)

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EGR2 Antibody (12-6691-82) in Flow

C57Bl/6 splenocytes stimulated overnight with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) were surface stained with Anti-Mouse CD19 PerCP-Cy5-5 (Product # 45-0193-82). Cells were then fixed and permeabilized using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and stained intracellularly with 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.125 µg of Anti-Mouse Egr2 PE (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

12-6691-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

erongr2

Immunogen

E. coli-derived protein, aa 1-319

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717804

Product Specific Information

Description: This erongr2 monoclonal antibody recognizes mouse early growth response 2 (Egr2, Krox-20). Egr2 is a zinc finger-containing transcription factor that is important for the development of T cells and NKT cells. Egr2 is predominantly expressed in the thymus and nervous system, and it is also upregulated in T cells following TCR-crosslinking. Upregulation in all non-adherent mouse splenocytes has been observed following stimulation with phorbol 12-myristate 13-acetate (PMA) and ionomycin. Egr2 is believed to antagonize T cell activation and has also been reported to promote apoptosis. The erongr2 antibody also appears to modestly stain a subpopulation of T cells and B cells in freshly isolated spleen cells. The erongr2 monoclonal antibody is specific for Egr2 and does not crossreact to Egr3, based on immunoblot analysis.

Applications Reported: This erongr2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This erongr2 antibody has been tested by intracellular flow cytometric analysis of stimulated mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The EGR2 gene provides instructions for making a protein called early growth response 2, which is part of the early growth response family of proteins. These proteins bind to specific areas of DNA and help control the activity of particular genes. On the basis of this action, the proteins are referred to as transcription factors. The early growth response 2 protein activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells. Myelin promotes the efficient transmission of nerve impulses. If myelin is lost (demyelination) or its structure is disrupted, the transmission of nerve impulses is impaired. Mutations in the EGR2 gene can cause two forms of Charcot-Marie-Tooth disease, type 1D or type 4E (sometimes called congenital hypomyelinating neuropathy) or a severe form of type 1D (sometimes called Dejerine-Sottas syndrome) that begins during infancy or early childhood.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

CD11c+ myeloid cell exosomes reduce intestinal inflammation during colitis.

JCI insight

Bauer KM,Nelson MC,Tang WW,Chiaro TR,Brown DG,Ghazaryan A,Lee SH,Weis AM,Hill JH,Klag KA,Tran VB,Thompson JW,Ramstead AG,Monts JK,Marvin JE,Alexander M,Voth WP,Stephens WZ,Ward DM,Petrey AC,Round JL,O'Connell RM

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Mon Oct 10 00:00:00 EDT 2022

Pro-inflammatory immunity supports fibrosis advancement in epidermolysis bullosa: intervention with Ang-(1-7).

EMBO molecular medicine

Bernasconi R,Thriene K,Romero-Fernández E,Gretzmeier C,Kühl T,Maler M,Nauroy P,Kleiser S,Rühl-Muth AC,Stumpe M,Kiritsi D,Martin SF,Hinz B,Bruckner-Tuderman L,Dengjel J,Nyström A

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Thu Oct 07 00:00:00 EDT 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Thu Dec 02 00:00:00 EST 2021

CAR-T cell-mediated depletion of immunosuppressive tumor-associated macrophages promotes endogenous antitumor immunity and augments adoptive immunotherapy.

Nature communications

Rodriguez-Garcia A,Lynn RC,Poussin M,Eiva MA,Shaw LC,O'Connor RS,Minutolo NG,Casado-Medrano V,Lopez G,Matsuyama T,Powell DJ

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Tue Feb 09 00:00:00 EST 2021

Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma.

Cell reports. Medicine

Chao JL,Korzinkin M,Zhavoronkov A,Ozerov IV,Walker MT,Higgins K,Lingen MW,Izumchenko E,Savage PA

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

Macrophages Expressing GALC Improve Peripheral Krabbe Disease by a Mechanism Independent of Cross-Correction.

Neuron

Weinstock NI,Shin D,Dhimal N,Hong X,Irons EE,Silvestri NJ,Reed CB,Nguyen D,Sampson O,Cheng YC,Lau JTY,Bongarzone ER,Kofler J,Escolar ML,Gelb MH,Wrabetz L,Feltri ML

12-6691 was used in Flow cytometry/Cell sorting to show that cross-correction does not occur efficiently in vivo and that Galc-deficient Schwann cells autonomously produce psychosine.

Wed Jul 08 00:00:00 EDT 2020

Mycobacterial Trehalose 6,6'-Dimycolate-Induced M1-Type Inflammation.

The American journal of pathology

Nguyen TKT,d'Aigle J,Chinea L,Niaz Z,Hunter RL,Hwang SA,Actor JK

Published figure using EGR2 monoclonal antibody (Product # 12-6691-82) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2020

Thymic iNKT single cell analyses unmask the common developmental program of mouse innate T cells.

Nature communications

Harsha Krovi S,Zhang J,Michaels-Foster MJ,Brunetti T,Loh L,Scott-Browne J,Gapin L

12-6691 was used in Flow cytometry/Cell sorting to highlight the common requirements for the development of innate-like T cells with a focus on how Hivep3 impacts the maturation of these lymphocytes.

Mon Dec 07 00:00:00 EST 2020

SHIP-1 Regulates Phagocytosis and M2 Polarization Through the PI3K/Akt-STAT5-Trib1 Circuit in Pseudomonas aeruginosa Infection.

Frontiers in immunology

Qin S,Li J,Zhou C,Privratsky B,Schettler J,Deng X,Xia Z,Zeng Y,Wu H,Wu M

12-6691-82 was used in Flow cytometry/Cell sorting to reveal a previously unrecognized role of SHIP-1 in inflammatory responses and macrophage homeostasis during P. aeruginosa infection through a PI3K/Akt-STAT5-Trib1 axis.

Thu Mar 25 00:00:00 EDT 2021

Transcription factors early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.

Immunity, inflammation and disease

Omodho B,Miao T,Symonds ALJ,Singh R,Li S,Wang P

12-6691 was used in Flow cytometry/Cell sorting to show that despite impaired proliferation and IL2 production, tolerant T cells can display inflammatory responses in response to antigen stimulation and this is controlled at least partly by Egr2 and 3.

Fri Jun 01 00:00:00 EDT 2018

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Mon Jun 05 00:00:00 EDT 2017

A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development.

Nature communications

Mao AP,Ishizuka IE,Kasal DN,Mandal M,Bendelac A

Mon Oct 16 00:00:00 EDT 2017

Adverse early life environment increases hippocampal microglia abundance in conjunction with decreased neural stem cells in juvenile mice.

International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience

Cohen S,Ke X,Liu Q,Fu Q,Majnik A,Lane R

12-6691 was used in Flow cytometry/Cell sorting to observe that adverse early life environment (AELE) significantly decreased neural stem cell and neuronal numbers in juvenile mice.

Thu Dec 01 00:00:00 EST 2016

TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.

Nature communications

Okamura T,Sumitomo S,Morita K,Iwasaki Y,Inoue M,Nakachi S,Komai T,Shoda H,Miyazaki J,Fujio K,Yamamoto K

12-6691 was used in Flow cytometry/Cell sorting to investigate the role of GF-β3-expressing CD4+CD25(-)LAG3+ Tregs in humoral immune responses.

Thu Feb 19 00:00:00 EST 2015

Egr-2 transcription factor is required for Blimp-1-mediated IL-10 production in IL-27-stimulated CD4+ T cells.

European journal of immunology

Iwasaki Y,Fujio K,Okamura T,Yanai A,Sumitomo S,Shoda H,Tamura T,Yoshida H,Charnay P,Yamamoto K

Mon Apr 01 00:00:00 EDT 2013

Elevated and sustained expression of the transcription factors Egr1 and Egr2 controls NKT lineage differentiation in response to TCR signaling.

Nature immunology

Seiler MP,Mathew R,Liszewski MK,Spooner CJ,Barr K,Meng F,Singh H,Bendelac A

Sun Feb 05 00:00:00 EST 2012

Bioinformatics

Protein Aliases: DKFZp686J1957; E3 SUMO-protein ligase EGR2; E3 SUMO-protein transferase ERG2; Early growth response protein 2; EGR-2; FLJ1454; FLJ14547; Krox-20 homolog; OTTHUMP00000062133; Zinc finger protein Krox-20

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Gene Aliases: Egr-2; Egr2; Krox-20; Krox20; NGF1-B; Zfp-25; Zfp-6

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UniProt ID: (Mouse) P08152

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Entrez Gene ID: (Mouse) 13654

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II activating transcription factor binding nucleic acid binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ligase activity ribosomal S6-glutamic acid ligase activity ubiquitin protein ligase binding coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity transcription regulatory region DNA binding metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity HMG box domain binding C2H2 zinc finger transcription factor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein export from nucleus rhythmic behavior motor neuron axon guidance Schwann cell differentiation rhombomere 3 development facial nerve structural organization rhombomere 3 formation rhombomere 5 formation regulation of ossification response to insulin brain segmentation skeletal muscle cell differentiation myelination negative regulation of apoptotic process fat cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of neuronal synaptic plasticity cellular response to organic substance cellular response to cAMP cellular response to gonadotropin stimulus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EGR2 Monoclonal Antibody (erongr2), PE, eBioscience™

EGR2 Antibody (12-6691-82) in Flow

Product Details

12-6691-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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