Invitrogen

EOMES Monoclonal Antibody (Dan11mag), PE-Cyanine5, eBioscience™

FIGURE: 1 / 19

EOMES Antibody (15-4875-82) in Flow

C57BL/6 splenocytes were stained with NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82), followed by intracellular staining with 0.25 µg of Rat IgG2a kappa Isotype Control, PE-Cyanine5 (Product # 15-4321-82) (left) or 0.25 µg of EOMES Monoclonal Antibody, PE-Cyanine5 (right) using Foxp3/Transcription Factor Buffer Staining Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

EOMES Antibody in Immunocytochemistry (ICC/IF)

EOMES Antibody in Immunohistochemistry (IHC)

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Product Details

15-4875-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Dan11mag

Conjugate

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

Excitation/Emission Max

568/666 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2802207

Product Specific Information

Description: This Dan11mag antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This DAN11MAG antibody has been reported for use in flow cytometric analysis.

Applications Tested: This DAN11MAG antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Aiolos represses CD4⁺ T cell cytotoxic programming via reciprocal regulation of T_FH transcription factors and IL-2 sensitivity.

Nature communications

Read KA,Jones DM,Pokhrel S,Hales EDS,Varkey A,Tuazon JA,Eisele CD,Abdouni O,Saadey A,Leonard MR,Warren RT,Powell MD,Boss JM,Hemann EA,Yount JS,Xin G,Ghoneim HE,Lio CJ,Freud AG,Collins PL,Oestreich KJ

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Fri Mar 24 00:00:00 EDT 2023

PTP1B Is an Intracellular Checkpoint that Limits T-cell and CAR T-cell Antitumor Immunity.

Cancer discovery

Wiede F,Lu KH,Du X,Zeissig MN,Xu R,Goh PK,Xirouchaki CE,Hogarth SJ,Greatorex S,Sek K,Daly RJ,Beavis PA,Darcy PK,Tonks NK,Tiganis T

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Neoantigen vaccine-induced CD4 T cells confer protective immunity in a mouse model of multiple myeloma through activation of CD8 T cells against non-vaccine, tumor-associated antigens.

Journal for immunotherapy of cancer

Bekri S,Rodney-Sandy R,Gruenstein D,Mei A,Bogen B,Castle J,Levey D,Cho HJ

15-4875-82 was used in Flow cytometry/Cell sorting to indicate that activation of CD8 CTL against MOPC315-associated antigens not present in the vaccine is one of the major mechanisms of tumor immunity.

Tue Feb 01 00:00:00 EST 2022

BAF subunit switching regulates chromatin accessibility to control cell cycle exit in the developing mammalian cortex.

Genes & development

Braun SMG,Petrova R,Tang J,Krokhotin A,Miller EL,Tang Y,Panagiotakos G,Crabtree GR

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

CD8⁺ T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis.

Cell death & disease

Feizi N,Focaccetti C,Pacella I,Tucci G,Rossi A,Costanza M,Pedotti R,Sidney J,Sette A,La Rocca C,Procaccini C,Matarese G,Barnaba V,Piconese S

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis.

PLoS genetics

Rashidieh B,Shohayeb B,Bain AL,Fortuna PRJ,Sinha D,Burgess A,Mills R,Adams RC,Lopez JA,Blumbergs P,Finnie J,Kalimutho M,Piper M,Hudson JE,Ng DCH,Khanna KK

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Immunocytochemistry

Fri Oct 01 00:00:00 EDT 2021

Stage-dependent effects of intermittent hypoxia influence the outcome of hippocampal adult neurogenesis.

Scientific reports

Khuu MA,Nallamothu T,Castro-Rivera CI,Arias-Cavieres A,Szujewski CC,Garcia Iii AJ

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Immunocytochemistry

Tue Mar 16 00:00:00 EDT 2021

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus.

International journal of molecular sciences

Aschman T,Schaffer S,Biniaris Georgallis SI,Triantafyllopoulou A,Staeheli P,Voll RE

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation.

JCI insight

Hope HC,Brownlie RJ,Fife CM,Steele L,Lorger M,Salmond RJ

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy.

The Journal of experimental medicine

Oda SK,Anderson KG,Ravikumar P,Bonson P,Garcia NM,Jenkins CM,Zhuang S,Daman AW,Chiu EY,Bates BM,Greenberg PD

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Tue Sep 22 00:00:00 EDT 2020

Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors.

Cell

Ponzetta A,Carriero R,Carnevale S,Barbagallo M,Molgora M,Perucchini C,Magrini E,Gianni F,Kunderfranco P,Polentarutti N,Pasqualini F,Di Marco S,Supino D,Peano C,Cananzi F,Colombo P,Pilotti S,Alomar SY,Bonavita E,Galdiero MR,Garlanda C,Mantovani A,Jaillon S

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Thu Jul 11 00:00:00 EDT 2019

EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming.

Nature communications

Istaces N,Splittgerber M,Lima Silva V,Nguyen M,Thomas S,Le A,Achouri Y,Calonne E,Defrance M,Fuks F,Goriely S,Azouz A

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Wed Jul 24 00:00:00 EDT 2019

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 15-4875-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

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Gene Aliases: C77258; Eomes; TBR-2; Tbr2

View more View less

UniProt ID: (Mouse) O54839

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Entrez Gene ID: (Mouse) 13813

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification blastocyst development trophectodermal cell differentiation CD8-positive, alpha-beta T cell differentiation involved in immune response immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation brain development cardioblast differentiation regulation of gene expression stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation cell differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EOMES Monoclonal Antibody (Dan11mag), PE-Cyanine5, eBioscience™

EOMES Antibody (15-4875-82) in Flow

Product Details

15-4875-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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