Invitrogen

EOMES Monoclonal Antibody (Dan11mag), eFluor™ 660, eBioscience™

FIGURE: 1 / 50

EOMES Antibody (50-4875-82) in Flow

Surface staining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 FITC (Product # 11-5941-82), followed by fixation and permeabilization with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and intracellular staining with 0.125 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (left) or 0.125 µg of Anti-Mouse EOMES eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.

EOMES Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

EOMES Antibody in Immunocytochemistry (ICC/IF)

EOMES Antibody in Immunohistochemistry (Frozen) (IHC (F))

EOMES Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

50-4875-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 12 publications 12 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 7 publications 7 publications

Flow Cytometry (Flow)

0.25 µg/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

Dan11mag

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574227

Product Specific Information

Description: This Dan11mag antibody recognizes Eomesodermin (EOMES), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This Dan11mag antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This Dan11mag antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

Aiolos represses CD4⁺ T cell cytotoxic programming via reciprocal regulation of T_FH transcription factors and IL-2 sensitivity.

Nature communications

Read KA,Jones DM,Pokhrel S,Hales EDS,Varkey A,Tuazon JA,Eisele CD,Abdouni O,Saadey A,Leonard MR,Warren RT,Powell MD,Boss JM,Hemann EA,Yount JS,Xin G,Ghoneim HE,Lio CJ,Freud AG,Collins PL,Oestreich KJ

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Fri Mar 24 00:00:00 EDT 2023

PTP1B Is an Intracellular Checkpoint that Limits T-cell and CAR T-cell Antitumor Immunity.

Cancer discovery

Wiede F,Lu KH,Du X,Zeissig MN,Xu R,Goh PK,Xirouchaki CE,Hogarth SJ,Greatorex S,Sek K,Daly RJ,Beavis PA,Darcy PK,Tonks NK,Tiganis T

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Tissue-specific transcriptional profiles and heterogeneity of natural killer cells and group 1 innate lymphoid cells.

Cell reports. Medicine

Lopes N,Galluso J,Escalière B,Carpentier S,Kerdiles YM,Vivier E

50-4875-82 was used in Flow cytometry/Cell sorting to reveal that the expressions of EOMES, GZMA, IRF8, JAK1, NKG7, PLEK, PRF1, and ZEB2 define NK cells and that IL7R, LTB, and RGS1 differentiate ILC1s from NK cells in mice and humans.

Tue Nov 15 00:00:00 EST 2022

BAF subunit switching regulates chromatin accessibility to control cell cycle exit in the developing mammalian cortex.

Genes & development

Braun SMG,Petrova R,Tang J,Krokhotin A,Miller EL,Tang Y,Panagiotakos G,Crabtree GR

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

CD8⁺ T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis.

Cell death & disease

Feizi N,Focaccetti C,Pacella I,Tucci G,Rossi A,Costanza M,Pedotti R,Sidney J,Sette A,La Rocca C,Procaccini C,Matarese G,Barnaba V,Piconese S

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis.

PLoS genetics

Rashidieh B,Shohayeb B,Bain AL,Fortuna PRJ,Sinha D,Burgess A,Mills R,Adams RC,Lopez JA,Blumbergs P,Finnie J,Kalimutho M,Piper M,Hudson JE,Ng DCH,Khanna KK

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunocytochemistry

Fri Oct 01 00:00:00 EDT 2021

Cep55 regulation of PI3K/Akt signaling is required for neocortical development and ciliogenesis.

PLoS genetics

Rashidieh B,Shohayeb B,Bain AL,Fortuna PRJ,Sinha D,Burgess A,Mills R,Adams RC,Lopez JA,Blumbergs P,Finnie J,Kalimutho M,Piper M,Hudson JE,Ng DCH,Khanna KK

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunocytochemistry

Fri Oct 01 00:00:00 EDT 2021

LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis.

Nature communications

Hu HB,Song ZQ,Song GP,Li S,Tu HQ,Wu M,Zhang YC,Yuan JF,Li TT,Li PY,Xu YL,Shen XL,Han QY,Li AL,Zhou T,Chun J,Zhang XM,Li HY

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry

Thu Jan 28 00:00:00 EST 2021

Stage-dependent effects of intermittent hypoxia influence the outcome of hippocampal adult neurogenesis.

Scientific reports

Khuu MA,Nallamothu T,Castro-Rivera CI,Arias-Cavieres A,Szujewski CC,Garcia Iii AJ

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunocytochemistry

Tue Mar 16 00:00:00 EDT 2021

Interferon Lambda Regulates Cellular and Humoral Immunity in Pristane-Induced Lupus.

International journal of molecular sciences

Aschman T,Schaffer S,Biniaris Georgallis SI,Triantafyllopoulou A,Staeheli P,Voll RE

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Fri Oct 29 00:00:00 EDT 2021

Heterologous arenavirus vector prime-boost overrules self-tolerance for efficient tumor-specific CD8 T cell attack.

Cell reports. Medicine

Bonilla WV,Kirchhammer N,Marx AF,Kallert SM,Krzyzaniak MA,Lu M,Darbre S,Schmidt S,Raguz J,Berka U,Vincenti I,Pauzuolis M,Kerber R,Hoepner S,Günther S,Magnus C,Merkler D,Orlinger KK,Zippelius A,Pinschewer DD

50-4875-82 was used in Flow Cytometry to establish an arenavirus-based immunotherapy regimen that allows reshuffling of immunodominance hierarchies and breaking self-directed tolerance for efficient tumor control.

Tue Mar 16 00:00:00 EDT 2021

Coordination of asparagine uptake and asparagine synthetase expression modulates CD8+ T cell activation.

JCI insight

Hope HC,Brownlie RJ,Fife CM,Steele L,Lorger M,Salmond RJ

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Centrosome anchoring regulates progenitor properties and cortical formation.

Nature

Shao W,Yang J,He M,Yu XY,Lee CH,Yang Z,Joyner AL,Anderson KV,Zhang J,Tsou MB,Shi H,Shi SH

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunocytochemistry

Wed Apr 01 00:00:00 EDT 2020

A Fas-4-1BB fusion protein converts a death to a pro-survival signal and enhances T cell therapy.

The Journal of experimental medicine

Oda SK,Anderson KG,Ravikumar P,Bonson P,Garcia NM,Jenkins CM,Zhuang S,Daman AW,Chiu EY,Bates BM,Greenberg PD

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Mon Dec 07 00:00:00 EST 2020

The Spindle-Associated Microcephaly Protein, WDR62, Is Required for Neurogenesis and Development of the Hippocampus.

Frontiers in cell and developmental biology

Shohayeb B,Ho UY,Hassan H,Piper M,Ng DCH

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry

Tue Oct 13 00:00:00 EDT 2020

Developmental exposure to diesel exhaust upregulates transcription factor expression, decreases hippocampal neurogenesis, and alters cortical lamina organization: relevance to neurodevelopmental disorders.

Journal of neurodevelopmental disorders

Cole TB,Chang YC,Dao K,Daza R,Hevner R,Costa LG

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry

Wed Dec 16 00:00:00 EST 2020

Loss of the neurodevelopmental disease-associated gene miR-146a impairs neural progenitor differentiation and causes learning and memory deficits.

Molecular autism

Fregeac J,Moriceau S,Poli A,Nguyen LS,Oury F,Colleaux L

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunocytochemistry

Mon Mar 30 00:00:00 EDT 2020

Loss of the neurodevelopmental disease-associated gene miR-146a impairs neural progenitor differentiation and causes learning and memory deficits.

Molecular autism

Fregeac J,Moriceau S,Poli A,Nguyen LS,Oury F,Colleaux L

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunocytochemistry

Mon Mar 30 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Tue Sep 22 00:00:00 EDT 2020

Intermediate progenitors support migration of neural stem cells into dentate gyrus outer neurogenic niches.

eLife

Nelson BR,Hodge RD,Daza RA,Tripathi PP,Arnold SJ,Millen KJ,Hevner RF

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry

Fri Apr 03 00:00:00 EDT 2020

Neutrophils Driving Unconventional T Cells Mediate Resistance against Murine Sarcomas and Selected Human Tumors.

Cell

Ponzetta A,Carriero R,Carnevale S,Barbagallo M,Molgora M,Perucchini C,Magrini E,Gianni F,Kunderfranco P,Polentarutti N,Pasqualini F,Di Marco S,Supino D,Peano C,Cananzi F,Colombo P,Pilotti S,Alomar SY,Bonavita E,Galdiero MR,Garlanda C,Mantovani A,Jaillon S

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Thu Jul 11 00:00:00 EDT 2019

An Id2^RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials.

Immunity

Xu W,Cherrier DE,Chea S,Vosshenrich C,Serafini N,Petit M,Liu P,Golub R,Di Santo JP

50-4875 was used in Flow cytometry/Cell sorting to suggest a revised model for ILC differentiation that redefines the cell-fate potential of helper-ILC-restricted Zbtb16+ ILCPs.

Tue Apr 16 00:00:00 EDT 2019

Aberrant calcium channel splicing drives defects in cortical differentiation in Timothy syndrome.

eLife

Panagiotakos G,Haveles C,Arjun A,Petrova R,Rana A,Portmann T,Paşca SP,Palmer TD,Dolmetsch RE

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunofluorescence

Mon Dec 23 00:00:00 EST 2019

EOMES interacts with RUNX3 and BRG1 to promote innate memory cell formation through epigenetic reprogramming.

Nature communications

Istaces N,Splittgerber M,Lima Silva V,Nguyen M,Thomas S,Le A,Achouri Y,Calonne E,Defrance M,Fuks F,Goriely S,Azouz A

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Wed Jul 24 00:00:00 EDT 2019

PI3K-Yap activity drives cortical gyrification and hydrocephalus in mice.

eLife

Roy A,Murphy RM,Deng M,MacDonald JW,Bammler TK,Aldinger KA,Glass IA,Millen KJ

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunofluorescence

Thu May 16 00:00:00 EDT 2019

Lineage does not regulate the sensory synaptic input of projection neurons in the mouse olfactory bulb.

eLife

Sánchez-Guardado L,Lois C

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunofluorescence

Tue Aug 27 00:00:00 EDT 2019

mTOR Modulates CD8+ T Cell Differentiation in Mice with Invasive Pulmonary Aspergillosis.

Open life sciences

Wang H,Xiao Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Helminth-induced IL-4 expands bystander memory CD8⁺ T cells for early control of viral infection.

Nature communications

Rolot M,Dougall AM,Chetty A,Javaux J,Chen T,Xiao X,Machiels B,Selkirk ME,Maizels RM,Hokke C,Denis O,Brombacher F,Vanderplasschen A,Gillet L,Horsnell WGC,Dewals BG

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Tue Oct 30 00:00:00 EDT 2018

Natural Killer Cells Promote Fetal Development through the Secretion of Growth-Promoting Factors.

Immunity

Fu B,Zhou Y,Ni X,Tong X,Xu X,Dong Z,Sun R,Tian Z,Wei H

50-4875-82 was used in Flow Cytometry to reveal properties of Natural Killer (NK) cells in promoting fetal growth.

Tue Dec 19 00:00:00 EST 2017

Meningeal retinoic acid contributes to neocortical lamination and radial migration during mouse brain development.

Biology open

Haushalter C,Schuhbaur B,Dollé P,Rhinn M

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry (Frozen)

Wed Feb 15 00:00:00 EST 2017

IL-12 Influence mTOR to Modulate CD8⁺ T Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.

International journal of medical sciences

Wang H,Li J,Han Q,Yang F,Xiao Y,Xiao M,Xu Y,Su L,Cui N,Liu D

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Mon May 21 00:00:00 EDT 2018

Active medulloblastoma enhancers reveal subgroup-specific cellular origins.

Nature

Lin CY,Erkek S,Tong Y,Yin L,Federation AJ,Zapatka M,Haldipur P,Kawauchi D,Risch T,Warnatz HJ,Worst BC,Ju B,Orr BA,Zeid R,Polaski DR,Segura-Wang M,Waszak SM,Jones DT,Kool M,Hovestadt V,Buchhalter I,Sieber L,Johann P,Chavez L,Gröschel S,Ryzhova M,Korshunov A,Chen W,Chizhikov VV,Millen KJ,Amstislavskiy V,Lehrach H,Yaspo ML,Eils R,Lichter P,Korbel JO,Pfister SM,Bradner JE,Northcott PA

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunofluorescence

Thu Feb 04 00:00:00 EST 2016

Tumor Interferon Signaling Regulates a Multigenic Resistance Program to Immune Checkpoint Blockade.

Cell

Benci JL,Xu B,Qiu Y,Wu TJ,Dada H,Twyman-Saint Victor C,Cucolo L,Lee DSM,Pauken KE,Huang AC,Gangadhar TC,Amaravadi RK,Schuchter LM,Feldman MD,Ishwaran H,Vonderheide RH,Maity A,Wherry EJ,Minn AJ

50-4875 was used in Flow cytometry/Cell sorting to demonstrate that prolonged interferon signalling orchestrates PDL1-dependent and PDL1-independent resistance to immune checkpoint blockade and to combinations such as radiation plus anti-CTLA4.

Thu Dec 01 00:00:00 EST 2016

Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity.

Science translational medicine

Dendrou CA,Cortes A,Shipman L,Evans HG,Attfield KE,Jostins L,Barber T,Kaur G,Kuttikkatte SB,Leach OA,Desel C,Faergeman SL,Cheeseman J,Neville MJ,Sawcer S,Compston A,Johnson AR,Everett C,Bell JI,Karpe F,Ultsch M,Eigenbrot C,McVean G,Fugger L

50-4875 was used in Flow cytometry/Cell sorting to reveal a protective homozygous effect that defined a signalling optimum between autoimmunity and immunodeficiency and identified TYK2 as a potential drug target for autoimmune disorders.

Wed Nov 02 00:00:00 EDT 2016

Cited2 Regulates Neocortical Layer II/III Generation and Somatosensory Callosal Projection Neuron Development and Connectivity.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Fame RM,MacDonald JL,Dunwoodie SL,Takahashi E,Macklis JD

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunocytochemistry

Wed Jun 15 00:00:00 EDT 2016

The G protein-coupled receptor GPR157 regulates neuronal differentiation of radial glial progenitors through the Gq-IP3 pathway.

Scientific reports

Takeo Y,Kurabayashi N,Nguyen MD,Sanada K

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunofluorescence

Wed May 04 00:00:00 EDT 2016

A Threshold Level of Intratumor CD8+ T-cell PD1 Expression Dictates Therapeutic Response to Anti-PD1.

Cancer research

Ngiow SF,Young A,Jacquelot N,Yamazaki T,Enot D,Zitvogel L,Smyth MJ

50-4875 was used in Flow cytometry/Cell sorting to provide a framework to interrogate intra-tumour CD8(+) T-cell PD1 and immune PDL1 levels and response in human cancer.

Tue Sep 15 00:00:00 EDT 2015

Type I interferons regulate eomesodermin expression and the development of unconventional memory CD8(+) T cells.

Nature communications

Martinet V,Tonon S,Torres D,Azouz A,Nguyen M,Kohler A,Flamand V,Mao CA,Klein WH,Leo O,Goriely S

50-4875 was used in Flow cytometry/Cell sorting to demonstrate that type I interferon signalling regulates the function and homeostasis of memory-like CD8(+) T cells.

Fri May 08 00:00:00 EDT 2015

Tbr2 deficiency in mitral and tufted cells disrupts excitatory-inhibitory balance of neural circuitry in the mouse olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mizuguchi R,Naritsuka H,Mori K,Mao CA,Klein WH,Yoshihara Y

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry

Wed Jun 27 00:00:00 EDT 2012

Tbr2 deficiency in mitral and tufted cells disrupts excitatory-inhibitory balance of neural circuitry in the mouse olfactory bulb.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mizuguchi R,Naritsuka H,Mori K,Mao CA,Klein WH,Yoshihara Y

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Immunohistochemistry

Wed Jun 27 00:00:00 EDT 2012

CD134 plus CD137 dual costimulation induces Eomesodermin in CD4 T cells to program cytotoxic Th1 differentiation.

Journal of immunology (Baltimore, Md. : 1950)

Qui HZ,Hagymasi AT,Bandyopadhyay S,St Rose MC,Ramanarasimhaiah R,Ménoret A,Mittler RS,Gordon SM,Reiner SL,Vella AT,Adler AJ

Published figure using EOMES monoclonal antibody (Product # 50-4875-82) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2011

IFN-alpha beta and self-MHC divert CD8 T cells into a distinct differentiation pathway characterized by rapid acquisition of effector functions.

Journal of immunology (Baltimore, Md. : 1950)

Marshall HD,Prince AL,Berg LJ,Welsh RM

Sun Aug 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

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Gene Aliases: C77258; Eomes; TBR-2; Tbr2

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UniProt ID: (Mouse) O54839

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Entrez Gene ID: (Mouse) 13813

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification blastocyst development trophectodermal cell differentiation CD8-positive, alpha-beta T cell differentiation involved in immune response immune system process transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter multicellular organism development gastrulation brain development cardioblast differentiation regulation of gene expression stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation cell differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EOMES Monoclonal Antibody (Dan11mag), eFluor™ 660, eBioscience™

EOMES Antibody (50-4875-82) in Flow

Product Details

50-4875-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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