Invitrogen

FAK Monoclonal Antibody (ZF002)

Advanced Verification

IP-mass spectrometry

5 Published Figures

11 References

View all (102) FAK antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FAK Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 8

FAK Antibody (39-6500) in ICC/IF

Immunofluorescent analysis of FAK was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes; permeabilized with 0.25% Triton™ X-100 for 10 minutes followed by blocking with 5% BSA for 1 hour at room temperature. The cells were incubated with FAK Mouse Monoclonal Antibody (Product # 39-6500) at 1 µgmL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes... View More

FAK Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

FAK Antibody — IP-MS enrichment of FAK (LFQ intensity): FAK was enriched 55-fold from LNCAP lysate compared to background proteins, using the optimized IP-MS workflow with Pierce MS-Compatible Magnetic IP Kit protein A/G (Product # 90409) and FAK antibody (Product # 39-6500). See more information on IP-MS verification of antibody selectivity. {IP-MS}

Product Details

39-6500

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1-2 µg/mL

View 4 publications 4 publications

ELISA (ELISA)

0.1-1 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

ZF002

Immunogen

Recombinant protein derived from the N-terminal region of the mouse and rat FAK-1 proteins

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533427

Product Specific Information

Mouse and rat FAK-1 proteins share 93% homology with human. On western blots, this antibody identifies the target band at ~110 kDa. Reactivity has been confirmed with HeLa cell lysates and mouse and rat brain homogenates by western blotting.

Target Information

Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Co-targeting FAK and Gli1 inhibits the tumor-associated macrophages-released CCL22-mediated esophageal squamous cell carcinoma malignancy.

MedComm

Chen J,Zhu Y,Zhao D,Zhang L,Zhang J,Xiao Y,Wu Q,Wang Y,Zhan Q

Published figure using FAK monoclonal antibody (Product # 39-6500) in Western Blot

Fri Dec 01 00:00:00 EST 2023

Mechanosensitive expression of lamellipodin promotes intracellular stiffness, cyclin expression and cell proliferation.

Journal of cell science

Brazzo JA,Biber JC,Nimmer E,Heo Y,Ying L,Zhao R,Lee K,Krause M,Bae Y

39-6500 was used in Western Blot to suggest that lamellipodin levels may be critical for regulating cell proliferation. This article has an associated First Person interview with the first author of the paper.

Tue Jun 15 00:00:00 EDT 2021

Phosphatidylinositol 4-kinase III beta regulates cell shape, migration, and focal adhesion number.

Molecular biology of the cell

Bilodeau P,Jacobsen D,Law-Vinh D,Lee JM

39-6500 was used in Western Blotting to show that the lipid kinase phosphatidylinositol 4-kinase III beta (PI4KIIIβ) regulates cell shape, migration, and focal adhesion (FA) number.

Sat Aug 01 00:00:00 EDT 2020

The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth.

eLife

Mondal B,Jin H,Kallappagoudar S,Sedkov Y,Martinez T,Sentmanat MF,Poet GJ,Li C,Fan Y,Pruett-Miller SM,Herz HM

39-6500 was used in Western Blotting to indicate a crucial role for the mitotic deacetylase complex (MiDAC) in regulating the ligands of the SLIT3 and NTN1 signaling axes to ensure the proper integrity of neurite development.

Thu Apr 16 00:00:00 EDT 2020

The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth.

eLife

Mondal B,Jin H,Kallappagoudar S,Sedkov Y,Martinez T,Sentmanat MF,Poet GJ,Li C,Fan Y,Pruett-Miller SM,Herz HM

Thu Apr 16 00:00:00 EDT 2020

Predicting gene expression using morphological cell responses to nanotopography.

Nature communications

Cutiongco MFA,Jensen BS,Reynolds PM,Gadegaard N

Published figure using FAK monoclonal antibody (Product # 39-6500) in Immunocytochemistry

Fri Mar 13 00:00:00 EDT 2020

HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl₂.

Scientific reports

Magdaleno C,Dixon L,Rajasekaran N,Varadaraj A

39-6500 was used in Immunocytochemistry-immunoflourescence to systematically reexamine the role of hypoxia mimetics as experimental substitutes for hypoxia and provides new findings on HIFα stabilization and the FN matrix in the context of renal cancer.

Thu Oct 29 00:00:00 EDT 2020

HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl₂.

Scientific reports

Magdaleno C,Dixon L,Rajasekaran N,Varadaraj A

Thu Oct 29 00:00:00 EDT 2020

HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoCl₂.

Scientific reports

Magdaleno C,Dixon L,Rajasekaran N,Varadaraj A

Thu Oct 29 00:00:00 EDT 2020

mDia1 and Cdc42 Regulate Activin B-Induced Migration of Bone Marrow-Derived Mesenchymal Stromal Cells.

Stem cells (Dayton, Ohio)

Wang X,Tang P,Guo F,Zhang M,Yan Y,Huang M,Chen Y,Zhang L,Zhang L

39-6500 was used in Western Blotting to study the role of RoA effectors in bone marrow derived mesenchymal stromal cell migration.

Tue Jan 01 00:00:00 EST 2019

Rhodoptilometrin, a Crinoid-Derived Anthraquinone, Induces Cell Regeneration by Promoting Wound Healing and Oxidative Phosphorylation in Human Gingival Fibroblast Cells.

Marine drugs

Tseng CC,Lai YC,Kuo TJ,Su JH,Sung PJ,Feng CW,Lin YY,Chen PC,Tai MH,Cheng SY,Kuo HM,Wen ZH

39-6500 was used in Immunocytochemistry to speculate that (+)-rhodoptilometrin might increase FAK expression and promote mitochondrial function to affect cell migration and promote gingival regeneration.

Wed Feb 27 00:00:00 EST 2019

Cell circuits between B cell progenitors and IL-7⁺ mesenchymal progenitor cells control B cell development.

The Journal of experimental medicine

Fistonich C,Zehentmeier S,Bednarski JJ,Miao R,Schjerven H,Sleckman BP,Pereira JP

39-6500 was used in Immunocytochemistry-immunoflourescence to determine how B cell progenitors regulate access to paracrine signals provided by bone marrow stromal cells for proliferation and survival.

Mon Oct 01 00:00:00 EDT 2018

Knockdown of glucose-regulated protein 78 decreases the invasion, metalloproteinase expression and ECM degradation in hepatocellular carcinoma cells.

Journal of experimental & clinical cancer research : CR

Li H,Song H,Luo J,Liang J,Zhao S,Su R

39-6500 was used in western blot to test if GRP78 knockdown affects extracellular matrix degradation.

Mon Apr 30 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2

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Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2

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UniProt ID: (Human) Q05397, (Mouse) Q5DTH7, (Rat) O35346

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Entrez Gene ID: (Human) 5747, (Mouse) 14083, (Rat) 25614

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Function(s)

actin binding protein kinase activity protein tyrosine kinase activity non-membrane spanning protein tyrosine kinase activity signal transducer activity Ras guanyl-nucleotide exchange factor activity receptor binding protein binding ATP binding JUN kinase binding protein kinase binding SH2 domain binding nucleotide binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding identical protein binding phosphatidylinositol 3-kinase binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FAK Monoclonal Antibody (ZF002)

FAK Antibody (39-6500) in ICC/IF

Product Details

39-6500

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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