Product References
Prolonged activity of the transposase helper may raise safety concerns during DNA transposon-based gene therapy.
Molecular therapy. Methods & clinical development
Imre G,Takács B,Czipa E,Drubi AB,Jaksa G,Latinovics D,Nagy A,Karkas R,Hudoba L,Vásárhelyi BM,Pankotai-Bodó G,Blastyák A,Hegedűs Z,Germán P,Bálint B,Ahmed Abdullah KS,Kopasz AG,Kovács A,Nagy LG,Sükösd F,Pintér L,Rülicke T,Barta E,Nagy I,Haracska L,Mátés L
MA5-15738-HRP was used in Western Blotting to reveal that the piggyBac transposase protein exhibits prolonged activity, which predicts the risk of oncogenesis by generating chromosomal double-strand breaks.
Thu Jun 08 00:00:00 EDT 2023
A Dual-Function "TRE-Lox" System for Genetic Deletion or Reversible, Titratable, and Near-Complete Downregulation of Cathepsin D.
International journal of molecular sciences
Terron HM,Maranan DS,Burgard LA,LaFerla FM,Lane S,Leissring MA
MA5-15738-HRP was used in Western Blotting to show that CatD expression can be disrupted by as much as 98% in mouse embryonic fibroblasts (MEFs).
Tue Apr 04 00:00:00 EDT 2023
Oxidized low density lipoprotein in the liver causes decreased permeability of liver lymphatic- but not liver sinusoidal-endothelial cells via VEGFR-3 regulation of VE-Cadherin.
Frontiers in physiology
Goldberg AR,Ferguson M,Pal S,Cohen R,Orlicky DJ,McCullough RL,Rutkowski JM,Burchill MA,Tamburini BAJ
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Tue Feb 14 00:00:00 EST 2023
Oxidized Low-Density Lipoprotein Drives Dysfunction of the Liver Lymphatic System.
Cellular and molecular gastroenterology and hepatology
Burchill MA,Finlon JM,Goldberg AR,Gillen AE,Dahms PA,McMahan RH,Tye A,Winter AB,Reisz JA,Bohrnsen E,Schafer JB,D'Alessandro A,Orlicky DJ,Kriss MS,Rosen HR,McCullough RL,Jirón Tamburini BA
MA5-15738-HRP was used in Western Blotting to identify oxLDL as a major contributor to decreased lymphatic permeability in the liver, a change which is consistent with decreased protein homeostasis and increased inflammation during chronic liver disease.
Mon Dec 20 00:00:00 EST 2021
Oxidized Low-Density Lipoprotein Drives Dysfunction of the Liver Lymphatic System.
Cellular and molecular gastroenterology and hepatology
Burchill MA,Finlon JM,Goldberg AR,Gillen AE,Dahms PA,McMahan RH,Tye A,Winter AB,Reisz JA,Bohrnsen E,Schafer JB,D'Alessandro A,Orlicky DJ,Kriss MS,Rosen HR,McCullough RL,Jirón Tamburini BA
MA5-15738-HRP was used in Western Blotting to identify oxLDL as a major contributor to decreased lymphatic permeability in the liver, a change which is consistent with decreased protein homeostasis and increased inflammation during chronic liver disease.
Mon Dec 20 00:00:00 EST 2021
DNA repair enzyme NEIL3 enables a stable neural representation of space by shaping transcription in hippocampal neurons.
iScience
Kunath N,Bugaj AM,Bigonah P,Fernandez-Berrocal MS,Bjørås M,Ye J
MA5-15738-HRP was used in Western Blot to identify a role of the DNA repair enzyme NEIL3 in modulating the maturation and function of hippocampal CA1 neurons by shaping the CA1 transcriptome during postnatal development and in association with spatial behavior.
Fri Dec 17 00:00:00 EST 2021
MYC regulates ribosome biogenesis and mitochondrial gene expression programs through its interaction with host cell factor-1.
eLife
Popay TM,Wang J,Adams CM,Howard GC,Codreanu SG,Sherrod SD,McLean JA,Thomas LR,Lorey SL,Machida YJ,Weissmiller AM,Eischen CM,Liu Q,Tansey WP
MA5-15738-HRP was used in Western Blot to define HCF-1 as a critical MYC co-factor.
Fri Jan 08 00:00:00 EST 2021
Neuroimmune and Mu-Opioid Receptor Alterations in the Mesocorticolimbic System in a Sex-Dependent Inflammatory Pain-Induced Alcohol Relapse-Like Rat Model.
Frontiers in immunology
Cuitavi J,Lorente JD,Campos-Jurado Y,Polache A,Hipólito L
MA5-15738-HRP was used in Western Blotting to study neuroinflammatory states and microglial activation in the mesocorticolimbic system during the abstinence and relapse phases.
Wed Oct 27 00:00:00 EDT 2021
NFAT signaling in human mesenchymal stromal cells affects extracellular matrix remodeling and antifungal immune responses.
iScience
Tidu F,De Zuani M,Jose SS,Bendíčková K,Kubala L,Caruso F,Cavalieri F,Forte G,Frič J
MA5-15738-HRP was used in Western Blot to reveal a dual mechanism underlying the MSC response to zymosan: while NF-κB directly controls inflammatory cytokine expression, NFAT impacts immune-cell functions by regulating ECM remodeling.
Fri Jun 25 00:00:00 EDT 2021
ACTRIIA-Fc rebalances activin/GDF versus BMP signaling in pulmonary hypertension.
Science translational medicine
Yung LM,Yang P,Joshi S,Augur ZM,Kim SSJ,Bocobo GA,Dinter T,Troncone L,Chen PS,McNeil ME,Southwood M,Poli de Frias S,Knopf J,Rosas IO,Sako D,Pearsall RS,Quisel JD,Li G,Kumar R,Yu PB
MA5-15738-HRP was used in Western Blotting to reveal an unexpectedly prominent role of GDF8, GDF11, and activin as drivers of pulmonary vascular disease and represents a therapeutic strategy for restoring the balance between SMAD1/5/9 and SMAD2/3 signaling in PAH.
Wed May 13 00:00:00 EDT 2020
miR-29a Is Repressed by MYC in Pancreatic Cancer and Its Restoration Drives Tumor-Suppressive Effects via Downregulation of LOXL2.
Molecular cancer research : MCR
Dey S,Kwon JJ,Liu S,Hodge GA,Taleb S,Zimmers TA,Wan J,Kota J
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Sat Feb 01 00:00:00 EST 2020
MiR-33a-5p inhibits the growth and metastasis of melanoma cells by targeting SNAI2.
Neoplasma
Zhang ZR,Yang N
MA5-15738-HRP was used in Western Blotting to study how miR-33a-5p repressed the progression of melanoma by targeting SNAI2 via inactivation of the PI3K/AKT/mTOR signaling pathway, providing a potential molecular mechanism for the treatment of melanoma.
Wed Jul 01 00:00:00 EDT 2020
PD-L1 Reverse Signaling in Dermal Dendritic Cells Promotes Dendritic Cell Migration Required for Skin Immunity.
Cell reports
Lucas ED,Schafer JB,Matsuda J,Kraus M,Burchill MA,Tamburini BAJ
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Tue Oct 13 00:00:00 EDT 2020
PD-L1 Reverse Signaling in Dermal Dendritic Cells Promotes Dendritic Cell Migration Required for Skin Immunity.
Cell reports
Lucas ED,Schafer JB,Matsuda J,Kraus M,Burchill MA,Tamburini BAJ
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Tue Oct 13 00:00:00 EDT 2020
Dietary nitrate attenuates high-fat diet-induced obesity via mechanisms involving higher adipocyte respiration and alterations in inflammatory status.
Redox biology
Peleli M,Ferreira DMS,Tarnawski L,McCann Haworth S,Xuechen L,Zhuge Z,Newton PT,Massart J,Chagin AS,Olofsson PS,Ruas JL,Weitzberg E,Lundberg JO,Carlström M
MA5-15738-HRP was used in Western Blotting to explore mechanisms involved in the effects of dietary nitrate on the metabolic dysfunctions induced by high-fat diet in mice.
Wed Jan 01 00:00:00 EST 2020
Dietary nitrate attenuates high-fat diet-induced obesity via mechanisms involving higher adipocyte respiration and alterations in inflammatory status.
Redox biology
Peleli M,Ferreira DMS,Tarnawski L,McCann Haworth S,Xuechen L,Zhuge Z,Newton PT,Massart J,Chagin AS,Olofsson PS,Ruas JL,Weitzberg E,Lundberg JO,Carlström M
MA5-15738-HRP was used in Western Blotting to explore mechanisms involved in the effects of dietary nitrate on the metabolic dysfunctions induced by high-fat diet in mice.
Wed Jan 01 00:00:00 EST 2020
Cyclin N-Terminal Domain-Containing-1 Coordinates Meiotic Crossover Formation with Cell-Cycle Progression in a Cyclin-Independent Manner.
Cell reports
Gray S,Santiago ER,Chappie JS,Cohen PE
MA5-15738-HRP was used in Western Blotting to demonstrate the role for CNTD1 as a stop-go regulator during prophase I.
Tue Jul 07 00:00:00 EDT 2020
RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells.
eLife
Tullett KM,Tan PS,Park HY,Schittenhelm RB,Michael N,Li R,Policheni AN,Gruber E,Huang C,Fulcher AJ,Danne JC,Czabotar PE,Wakim LM,Mintern JD,Ramm G,Radford KJ,Caminschi I,O'Keeffe M,Villadangos JA,Wright MD,Blewitt ME,Heath WR,Shortman K,Purcell AW,Nicola NA,Zhang JG,Lahoud MH
MA5-15738-HRP was used in Western Blotting to show that this process is regulated by E3 ubiquitin ligase RNF41 and define a new ubiquitin-mediated mechanism for regulation of Clec9A, reflecting the unique properties of Clec9A as a receptor specialized for delivery of antigens for cross-presentation.
Wed Dec 02 00:00:00 EST 2020
Kainate Receptor Activation Shapes Short-Term Synaptic Plasticity by Controlling Receptor Lateral Mobility at Glutamatergic Synapses.
Cell reports
Polenghi A,Nieus T,Guazzi S,Gorostiza P,Petrini EM,Barberis A
MA5-15738-HRP was used in Western Blotting to study the crosstalk between conformational states and lateral mobility of KARs, a mechanism regulating glutamatergic signaling, particularly in conditions of sustained synaptic activity.
Tue Jun 09 00:00:00 EDT 2020
Altered expression of CYP17A1 and CYP19A1 in undescended testes of dogs with unilateral cryptorchidism.
Animal genetics
Krzeminska P,Stachowiak M,Skrzypski M,Nowak T,Maslak A,Switonski M
MA5-15738-HRP was used in Western Blotting to show that the identified polymorphisms cannot be considered markers associated with a predisposition to cryptorchidism.
Thu Oct 01 00:00:00 EDT 2020
Inhalation of lung spheroid cell secretome and exosomes promotes lung repair in pulmonary fibrosis.
Nature communications
Dinh PC,Paudel D,Brochu H,Popowski KD,Gracieux MC,Cores J,Huang K,Hensley MT,Harrell E,Vandergriff AC,George AK,Barrio RT,Hu S,Allen TA,Blackburn K,Caranasos TG,Peng X,Schnabel LV,Adler KB,Lobo LJ,Goshe MB,Cheng K
MA5-15738-HRP was used in Western Blotting to show that an inhalation treatment of secretome and exosome exhibited therapeutic potential for lung regeneration in two experimental models of pulmonary fibrosis.
Fri Feb 28 00:00:00 EST 2020
Myostatin: a Circulating Biomarker Correlating with Disease in Myotubular Myopathy Mice and Patients.
Molecular therapy. Methods & clinical development
Koch C,Buono S,Menuet A,Robé A,Djeddi S,Kretz C,Gomez-Oca R,Depla M,Monseur A,Thielemans L,Servais L,Laporte J,Cowling BS
MA5-15738-HRP was used in Western Blotting to provide evidence of a blood-based biomarker that may be used to monitor disease state in XL-CNM mice and patients and supports monitoring circulating myostatin during clinical trials for myotubular myopathy.
Fri Jun 12 00:00:00 EDT 2020
Sleep-wake cycles drive daily dynamics of synaptic phosphorylation.
Science (New York, N.Y.)
Brüning F,Noya SB,Bange T,Koutsouli S,Rudolph JD,Tyagarajan SK,Cox J,Mann M,Brown SA,Robles MS
MA5-15738-HRP was used in Western Blotting to find that sleep deprivation abolished 98% of all phosphorylation cycles in synaptoneurosomes, indicating that sleep-wake cycles rather than circadian signals are main drivers of synaptic phosphorylation, responding to both sleep and wake pressures.
Fri Oct 11 00:00:00 EDT 2019
A Human iPSC Double-Reporter System Enables Purification of Cardiac Lineage Subpopulations with Distinct Function and Drug Response Profiles.
Cell stem cell
Zhang JZ,Termglinchan V,Shao NY,Itzhaki I,Liu C,Ma N,Tian L,Wang VY,Chang ACY,Guo H,Kitani T,Wu H,Lam CK,Kodo K,Sayed N,Blau HM,Wu JC
MA5-15738-HRP was used in Western Blotting to study the effects of hiPSC TBX5Clover2 and NKX2-5TagRFP double reporters in their ability to delineate cardiac lineages and isolate lineage-specific subpopulations.
Thu May 02 00:00:00 EDT 2019
Retapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial Proteome.
Molecular cell
Meydan S,Marks J,Klepacki D,Sharma V,Baranov PV,Firth AE,Margus T,Kefi A,Vázquez-Laslop N,Mankin AS
MA5-15738-HRP was used in Western Blotting to contribute to the alternative bacterial proteome by identifying new internal start sights in Escherichia coli genes using Retapamulin-enhanced Ribo-seq analysis.
Thu May 02 00:00:00 EDT 2019
Knockdown of BNIP3L or SQSTM1 alters cellular response to mitochondria target drugs.
Autophagy
Rodrigo R,Mendis N,Ibrahim M,Ma C,Kreinin E,Roma A,Berg S,Blay J,Spagnuolo PA
MA5-15738-HRP was used in Western Blotting to investigate the mitochondria-specific changes that occur in leukemia cells after knockdown of BNIP3L/Nix or SQSTM1/p62.
Wed May 01 00:00:00 EDT 2019
Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment.
Bosnian journal of basic medical sciences
Demirgan S,Akyol O,Temel Z,Şengelen A,Pekmez M,Demirgan R,Sevdi MS,Erkalp K,Selcan A
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Tue Aug 20 00:00:00 EDT 2019
Displacement of WDR5 from Chromatin by a WIN Site Inhibitor with Picomolar Affinity.
Cell reports
Aho ER,Wang J,Gogliotti RD,Howard GC,Phan J,Acharya P,Macdonald JD,Cheng K,Lorey SL,Lu B,Wenzel S,Foshage AM,Alvarado J,Wang F,Shaw JG,Zhao B,Weissmiller AM,Thomas LR,Vakoc CR,Hall MD,Hiebert SW,Liu Q,Stauffer SR,Fesik SW,Tansey WP
MA5-15738-HRP was used in Western Blotting to identify WIN site inhibitors and demonstrate that the WIN site links WDR5 to chromatin at a small cohort of loci, including a specific subset of ribosome protein genes.
Tue Mar 12 00:00:00 EDT 2019
Engineering DNA recognition and allosteric response properties of TetR family proteins by using a module-swapping strategy.
Nucleic acids research
Dimas RP,Jordan BR,Jiang XL,Martini C,Glavy JS,Patterson DP,Morcos F,Chan CTY
MA5-15738-HRP was used in Western Blotting to demonstrate that TetR family regulators contain discrete and functional modules that can be used to build biological components with novel properties.
Thu Sep 19 00:00:00 EDT 2019
RAB11FIP5 Expression and Altered Natural Killer Cell Function Are Associated with Induction of HIV Broadly Neutralizing Antibody Responses.
Cell
Bradley T,Peppa D,Pedroza-Pacheco I,Li D,Cain DW,Henao R,Venkat V,Hora B,Chen Y,Vandergrift NA,Overman RG,Edwards RW,Woods CW,Tomaras GD,Ferrari G,Ginsburg GS,Connors M,Cohen MS,Moody MA,Borrow P,Haynes BF
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Thu Oct 04 00:00:00 EDT 2018
Correction of cognitive deficits in mouse models of Down syndrome by a pharmacological inhibitor of DYRK1A.
Disease models & mechanisms
Nguyen TL,Duchon A,Manousopoulou A,Loaëc N,Villiers B,Pani G,Karatas M,Mechling AE,Harsan LA,Limanton E,Bazureau JP,Carreaux F,Garbis SD,Meijer L,Herault Y
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Thu Sep 27 00:00:00 EDT 2018
Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function.
Scientific reports
Cops J,Mullens W,Verbrugge FH,Swennen Q,De Moor B,Reynders C,Penders J,Achten R,Driessen A,Dendooven A,Rigo JM,Hansen D
MA5-15738-HRP was used in Western Blotting to study the consequences of isolated abdominal venous congestion on the morphology and function of the kidneys, liver and heart in a rat model.
Mon Dec 10 00:00:00 EST 2018
Selective abdominal venous congestion induces adverse renal and hepatic morphological and functional alterations despite a preserved cardiac function.
Scientific reports
Cops J,Mullens W,Verbrugge FH,Swennen Q,De Moor B,Reynders C,Penders J,Achten R,Driessen A,Dendooven A,Rigo JM,Hansen D
MA5-15738-HRP was used in Western Blotting to study the consequences of isolated abdominal venous congestion on the morphology and function of the kidneys, liver and heart in a rat model.
Mon Dec 10 00:00:00 EST 2018
Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m6A mRNA Methylation.
Cell reports
Zhong X,Yu J,Frazier K,Weng X,Li Y,Cham CM,Dolan K,Zhu X,Hubert N,Tao Y,Lin F,Martinez-Guryn K,Huang Y,Wang T,Liu J,He C,Chang EB,Leone V
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Tue Nov 13 00:00:00 EST 2018
Modification of N6-methyladenosine RNA methylation on heat shock protein expression.
PloS one
Yu J,Li Y,Wang T,Zhong X
MA5-15738-HRP was used in Western Blotting to investigate the effect of N6-methyladenosine RNA methylation on Heat shock proteins (HSPs), and dissect the profile of HSP RNA methylation.
Mon Dec 31 00:00:00 EST 2018
Novel differences in renal gene expression in a diet-induced obesity model.
American journal of physiology. Renal physiology
Halperin Kuhns VL,Pluznick JL
MA5-15738-HRP was used in Western Blotting to demonstrate that in a HF-diet model of early disease, novel transcriptional changes occur that are both sex specific and specific to the renal cortex.
Sun Apr 01 00:00:00 EDT 2018
Mineralocorticoid Antagonist Improves Glucocorticoid Receptor Signaling and Dexamethasone Analgesia in an Animal Model of Low Back Pain.
Frontiers in cellular neuroscience
Ibrahim SIA,Xie W,Strong JA,Tonello R,Berta T,Zhang JM
MA5-15738-HRP was used in Western Blotting to examine the effects of local Dexamethasone, and eplerenone applied to the dorsal root ganglia at the time of inflammation.
Thu Oct 01 00:00:00 EDT 2020
Low Frequency Magnetic Fields Induce Autophagy-associated Cell Death in Lung Cancer through miR-486-mediated Inhibition of Akt/mTOR Signaling Pathway.
Scientific reports
Xu Y,Wang Y,Yao A,Xu Z,Dou H,Shen S,Hou Y,Wang T
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Mon Sep 18 00:00:00 EDT 2017
Diamond, graphite, and graphene oxide nanoparticles decrease migration and invasiveness in glioblastoma cell lines by impairing extracellular adhesion.
International journal of nanomedicine
Wierzbicki M,Jaworski S,Kutwin M,Grodzik M,Strojny B,Kurantowicz N,Zdunek K,Chodun R,Chwalibog A,Sawosz E
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Fri Jan 26 00:00:00 EST 2018
YAP regulates cell mechanics by controlling focal adhesion assembly.
Nature communications
Nardone G,Oliver-De La Cruz J,Vrbsky J,Martini C,Pribyl J,Skládal P,Pešl M,Caluori G,Pagliari S,Martino F,Maceckova Z,Hajduch M,Sanz-Garcia A,Pugno NM,Stokin GB,Forte G
MA5-15738-HRP was used in Western Blotting to study the molecular mechanism by which cell spreading and RhoA GTPase activity control focal adhesion formation through YAP to stabilise the anchorage of the actin cytoskeleton to the cell membrane.
Mon May 15 00:00:00 EDT 2017
Deletions linked to TP53 loss drive cancer through p53-independent mechanisms.
Nature
Liu Y,Chen C,Xu Z,Scuoppo C,Rillahan CD,Gao J,Spitzer B,Bosbach B,Kastenhuber ER,Baslan T,Ackermann S,Cheng L,Wang Q,Niu T,Schultz N,Levine RL,Mills AA,Lowe SW
MA5-15738-HRP was used in western blot to assess the effect of 11B3 loss on tumorigenesis
Thu Mar 24 00:00:00 EDT 2016
Progress of endocytic CHRN to autophagic degradation is regulated by RAB5-GTPase and T145 phosphorylation of SH3GLB1 at mouse neuromuscular junctions in vivo.
Autophagy
Wild F,Khan MM,Straka T,Rudolf R
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Thu Dec 01 00:00:00 EST 2016
Protein-Protein Interactions Suggest Novel Activities of Human Cytomegalovirus Tegument Protein pUL103.
Journal of virology
Ortiz DA,Glassbrook JE,Pellett PE
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Thu Sep 01 00:00:00 EDT 2016
Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice.
American journal of physiology. Endocrinology and metabolism
Charrier A,Wang L,Stephenson EJ,Ghanta SV,Ko CW,Croniger CM,Bridges D,Buchner DA
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Tue Nov 01 00:00:00 EDT 2016
Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.
eLife
Zhang L,Tran NT,Su H,Wang R,Lu Y,Tang H,Aoyagi S,Guo A,Khodadadi-Jamayran A,Zhou D,Qian K,Hricik T,Côté J,Han X,Zhou W,Laha S,Abdel-Wahab O,Levine RL,Raffel G,Liu Y,Chen D,Li H,Townes T,Wang H,Deng H,Zheng YG,Leslie C,Luo M,Zhao X
Published figure using GAPDH Loading Control monoclonal antibody (Product # MA5-15738-HRP) in Western Blot
Tue Nov 17 00:00:00 EST 2015
Novel miRNA-31 and miRNA-200a-Mediated Regulation of Retinoblastoma Proliferation.
PloS one
Montoya V,Fan H,Bryar PJ,Weinstein JL,Mets MB,Feng G,Martin J,Martin A,Jiang H,Laurie NA
MA5-15738-HRP was used in Western Blotting to elucidate the roles of miR-31 and miR-200a in tumour proliferation.
Fri May 20 00:00:00 EDT 2016
