Invitrogen

GL7 Monoclonal Antibody (GL-7 (GL7)), Alexa Fluor™ 488, eBioscience™

18 Published Figures

26 References

View all (7) GL7 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (18)

FIGURE: 1 / 19

GL7 Antibody (53-5902-82) in Flow

Staining of unstimulated (left) or Con A-stimulated (right) C57Bl/6 splenocytes with Anti-Mouse CD3e PE (Product # 12-0031-82) and 0.25 µg of Anti-Human/Mouse GL7 (T and B Cell Activation Marker) Alexa Fluor® 488. Total viable cells, as determined by Fixable Viability Dye eFluor® 780 (Product # 65-0865-14) were used for analysis.

GL7 Antibody in Immunocytochemistry (ICC/IF)

GL7 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-5902-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.5 µg/test

View 19 publications 19 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgM

Recommended Isotype Control

Rat IgM Isotype Control (eBRM), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

GL-7 (GL7)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2016717

Product Specific Information

Description: This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

Applications Reported: This GL-7 (GL7) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This GL-7 (GL7) antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GL7 is a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac. This moiety is recognized by CD22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids.

ACS central science

Moeller TD,Shah SB,Lai K,Lopez-Barbosa N,Desai P,Wang W,Zhong Z,Redmond D,Singh A,DeLisa MP

53-5902-82 was used in Flow cytometry/Cell sorting to highlight the potential of synthetic organoids for rapidly predicting conjugate vaccine efficacy as well as expediting antigen-specific antibody discovery.

Wed Apr 26 00:00:00 EDT 2023

Microbiota-Derived Metabolites Suppress Arthritis by Amplifying Aryl-Hydrocarbon Receptor Activation in Regulatory B Cells.

Cell metabolism

Rosser EC,Piper CJM,Matei DE,Blair PA,Rendeiro AF,Orford M,Alber DG,Krausgruber T,Catalan D,Klein N,Manson JJ,Drozdov I,Bock C,Wedderburn LR,Eaton S,Mauri C

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Immunohistochemistry

Tue Apr 07 00:00:00 EDT 2020

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Immunohistochemistry

Mon Jun 03 00:00:00 EDT 2019

Activated Peyer's patch B cells sample antigen directly from M cells in the subepithelial dome.

Nature communications

Komban RJ,Strömberg A,Biram A,Cervin J,Lebrero-Fernández C,Mabbott N,Yrlid U,Shulman Z,Bemark M,Lycke N

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Immunohistochemistry

Mon Jun 03 00:00:00 EDT 2019

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

53-5902-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 EDT 2019

Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction.

Immunity

Li X,Gadzinsky A,Gong L,Tong H,Calderon V,Li Y,Kitamura D,Klein U,Langdon WY,Hou F,Zou YR,Gu H

53-5902-82 was used in Flow Cytometry to show that deleting E3 ubiquitin ligases Cbl and Cbl-b (Cbls) in GC B cells results in the early exit of high-affinity antigen-specific B cells from the GC reaction and thus impaired clonal expansion.

Tue Mar 20 00:00:00 EDT 2018

αv Integrins regulate germinal center B cell responses through noncanonical autophagy.

The Journal of clinical investigation

Raso F,Sagadiev S,Du S,Gage E,Arkatkar T,Metzler G,Stuart LM,Orr MT,Rawlings DJ,Jackson SW,Lacy-Hulbert A,Acharya M

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Flow Cytometry

Fri Aug 31 00:00:00 EDT 2018

Human herpesvirus-encoded kinase induces B cell lymphomas in vivo.

The Journal of clinical investigation

Anders PM,Montgomery ND,Montgomery SA,Bhatt AP,Dittmer DP,Damania B

53-5902 was used in Flow cytometry/Cell sorting to analyse the function of viral protein kinase in vivo.

Fri Jun 01 00:00:00 EDT 2018

RNA Helicase DDX1 Converts RNA G-Quadruplex Structures into R-Loops to Promote IgH Class Switch Recombination.

Molecular cell

Ribeiro de Almeida C,Dhir S,Dhir A,Moghaddam AE,Sattentau Q,Meinhart A,Proudfoot NJ

53-5902 was used in Flow cytometry/Cell sorting to provide evidence for how S-region transcripts interconvert between G4 and R-loop structures to promote IgH class switch recombination.

Thu May 17 00:00:00 EDT 2018

Anti‑IL‑39 (IL‑23p19/Ebi3) polyclonal antibodies ameliorate autoimmune symptoms in lupus‑like mice.

Molecular medicine reports

Wang X,Zhang Y,Wang Z,Liu X,Zhu G,Han G,Chen G,Hou C,Wang T,Shen B,Li Y,Xiao H,Ma N,Wang R

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

B cell activation and plasma cell differentiation are inhibited by de novo DNA methylation.

Nature communications

Barwick BG,Scharer CD,Martinez RJ,Price MJ,Wein AN,Haines RR,Bally APR,Kohlmeier JE,Boss JM

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Immunofluorescence

Tue May 15 00:00:00 EDT 2018

Act1 is a negative regulator in T and B cells via direct inhibition of STAT3.

Nature communications

Zhang CJ,Wang C,Jiang M,Gu C,Xiao J,Chen X,Martin BN,Tang F,Yamamoto E,Xian Y,Wang H,Li F,Sartor RB,Smith H,Husni ME,Shi FD,Gao J,Carman J,Dongre A,McKarns SC,Coppieters K,Jørgensen TN,Leonard WJ,Li X

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Flow Cytometry

Mon Jul 16 00:00:00 EDT 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

Deficiency in Calcium-Binding Protein S100A4 Impairs the Adjuvant Action of Cholera Toxin.

Frontiers in immunology

Sun JB,Holmgren J,Larena M,Terrinoni M,Fang Y,Bresnick AR,Xiang Z

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Immunofluorescence

Wed Nov 20 00:00:00 EST 2019

Loss of IP₃ Receptor-Mediated Ca²⁺ Release in Mouse B Cells Results in Abnormal B Cell Development and Function.

Journal of immunology (Baltimore, Md. : 1950)

Tang H,Wang H,Lin Q,Fan F,Zhang F,Peng X,Fang X,Liu J,Ouyang K

53-5902 was used in Flow cytometry/Cell sorting to determine the function of 1,4,5-trisphosphate receptor-mediated Ca2+ release in B cells.

Sat Jul 15 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Class-switched anti-insulin antibodies originate from unconventional antigen presentation in multiple lymphoid sites.

The Journal of experimental medicine

Wan X,Thomas JW,Unanue ER

53-5902 was used in Flow cytometry/Cell sorting to investigate the interactions of insulin-specific T and B lymphocytes using T cell and B cell receptor transgenic mice.

Mon May 30 00:00:00 EDT 2016

Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization.

Nature communications

Bemark M,Hazanov H,Strömberg A,Komban R,Holmqvist J,Köster S,Mattsson J,Sikora P,Mehr R,Lycke NY

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Immunofluorescence

Tue Sep 06 00:00:00 EDT 2016

The Extracellular Domains of IgG1 and T Cell-Derived IL-4/IL-13 Are Critical for the Polyclonal Memory IgE Response In Vivo.

PLoS biology

Turqueti-Neves A,Otte M,Schwartz C,Schmitt ME,Lindner C,Pabst O,Yu P,Voehringer D

53-5902 was used in Flow cytometry/Cell sorting to explore the relationship between repertoires of IgE and IgG1 in vivo.

Tue May 24 00:00:00 EDT 2016

BILL-cadherin/cadherin-17 contributes to the survival of memory B cells.

PloS one

Funakoshi S,Shimizu T,Numata O,Ato M,Melchers F,Ohnishi K

53-5902 was used in Flow cytometry/Cell sorting to study how CDH17 plays a role in the long-term survival of memory B cells via an 'MBC niche'.

Wed Sep 16 00:00:00 EDT 2015

CD40 ligand preferentially modulates immune response and enhances protection against influenza virus.

Journal of immunology (Baltimore, Md. : 1950)

Hashem AM,Gravel C,Chen Z,Yi Y,Tocchi M,Jaentschke B,Fan X,Li C,Rosu-Myles M,Pereboev A,He R,Wang J,Li X

53-5902 was used in Flow cytometry/Cell sorting to characterise an increased in vivo load of CD40-targeted Ag upon the secretion of the NP-CD40L fusion protein from adenovirus-infected cells.

Tue Jul 15 00:00:00 EDT 2014

Murine complement receptor 1 is required for germinal center B cell maintenance but not initiation.

Immunobiology

Donius LR,Weis JJ,Weis JH

53-5902 was used in Flow cytometry/Cell sorting to describe unique functions of Cr1 on the surface of both B cells and follicular dendritic cells.

Sun Jun 01 00:00:00 EDT 2014

Mutation of POLB causes lupus in mice.

Cell reports

Senejani AG,Liu Y,Kidane D,Maher SE,Zeiss CJ,Park HJ,Kashgarian M,McNiff JM,Zelterman D,Bothwell AL,Sweasy JB

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Flow Cytometry

Thu Jan 16 00:00:00 EST 2014

Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria.

The EMBO journal

Panda S,Zhang J,Tan NS,Ho B,Ding JL

Published figure using GL7 monoclonal antibody (Product # 53-5902-82) in Immunofluorescence

Wed Nov 13 00:00:00 EST 2013

TRAF binding is required for a distinct subset of in vivo B cell functions of the oncoprotein LMP1.

Journal of immunology (Baltimore, Md. : 1950)

Arcipowski KM,Bishop GA

53-5902 was used in Flow cytometry/Cell sorting to investigate whether the mechanism of action of TRAF proteins for LMP1 in vivo functions requires direct association.

Sat Dec 01 00:00:00 EST 2012

Human B-lymphocytes express alpha2-6-sialylated 6-sulfo-N-acetyllactosamine serving as a preferred ligand for CD22/Siglec-2.

The Journal of biological chemistry

Kimura N,Ohmori K,Miyazaki K,Izawa M,Matsuzaki Y,Yasuda Y,Takematsu H,Kozutsumi Y,Moriyama A,Kannagi R

Fri Nov 02 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: GL7 antigen; Ly-77; Ly77

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GL7 Monoclonal Antibody (GL-7 (GL7)), Alexa Fluor™ 488, eBioscience™

GL7 Antibody (53-5902-82) in Flow

Product Details

53-5902-82

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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