Invitrogen

GM-CSF Monoclonal Antibody (MP1-22E9), PE, eBioscience™

GM-CSF Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 4

GM-CSF Antibody (12-7331-82) in Flow

Mouse splenocytes were stimulated with ConA for 2 days, followed by Mouse IL-2 Recombinant Protein (Product # 14-8021-64) and Mouse IL-4 Recombinant Protein (Product # 14-8041-80) for 3 days, and restimulated with immobilized Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) and soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82) in the presence of Brefeldin A for 5 hours. The cells were surface stained with Anti-Mouse CD4 FITC and intracellularly stained with Anti-Mouse GM-CSF PE.

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Product Details

12-7331-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MP1-22E9

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466205

Product Specific Information

Description: The MP1-22E9 antibody reacts with mouse granulocyte/macrophage - colony stimulating factor (GM-CSF). The MP1-22E9 antibody is a neutralizing antibody. Mouse GM-CSF is a 14 kDa factor produced mainly by activated T cells and macrophages. Other cell types, such as endothelium and fibroblasts, also secrete GM-CSF in response to TNF-alpha, IL-2, IL-1, and IFN-gamma. GM-CSF stimulates growth of macrophages, granulocytes and dendritic cells. GM-CSF is found as a membrane-bound form and also as a complex associated with the extracellular matrix. Non-glycosylated GM-CSF is biologically active.

Applications Reported:MP1-22E9 has been reported for use in intracellular flow cytometric analysis.

Applications Tested: Has been tested bu intracellular staining and flow cytometric analysis of cultured mouse splencoytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The GM-CSF gene encodes for a cytokine that regulates the production, differentiation, and function of macrophages and granulocytes. The active form of the protein exists as a homodimer in the extracellular space. This gene is located in a cluster of related genes at the chromosome region 5q31, which has been associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include interleukins 4, 5, and 13. The gene is involved in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been observed in SARS-CoV-2 infected patients with acute respiratory distress syndrome. Mice that lack this gene or its receptor have been shown to develop pulmonary alveolar proteinosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Itaconate ameliorates autoimmunity by modulating T cell imbalance via metabolic and epigenetic reprogramming.

Nature communications

Aso K,Kono M,Kanda M,Kudo Y,Sakiyama K,Hisada R,Karino K,Ueda Y,Nakazawa D,Fujieda Y,Kato M,Amengual O,Atsumi T

Published figure using GM-CSF monoclonal antibody (Product # 12-7331-82) in Flow Cytometry

Mon Feb 27 00:00:00 EST 2023

SYK coordinates neuroprotective microglial responses in neurodegenerative disease.

Cell

Ennerfelt H,Frost EL,Shapiro DA,Holliday C,Zengeler KE,Voithofer G,Bolte AC,Lammert CR,Kulas JA,Ulland TK,Lukens JR

12-7331-82 was used in flow cytometry to study the critical intracellular molecules that orchestrate neuroprotective functions of microglia.

Thu Oct 27 00:00:00 EDT 2022

Cold exposure protects from neuroinflammation through immunologic reprogramming.

Cell metabolism

Spiljar M,Steinbach K,Rigo D,Suárez-Zamorano N,Wagner I,Hadadi N,Vincenti I,Page N,Klimek B,Rochat MA,Kreutzfeldt M,Chevalier C,Stojanović O,Bejuy O,Colin D,Mack M,Cansever D,Greter M,Merkler D,Trajkovski M

Published figure using GM-CSF monoclonal antibody (Product # 12-7331-82) in Flow Cytometry

Tue Nov 02 00:00:00 EDT 2021

Prevention of EAE by tolerogenic vaccination with PEGylated antigenic peptides.

Therapeutic advances in chronic disease

Pfeil J,Simonetti M,Lauer U,von Thülen B,Durek P,Poulsen C,Pawlowska J,Kröger M,Krähmer R,Leenders F,Hoffmann U,Hamann A

Published figure using GM-CSF monoclonal antibody (Product # 12-7331-82) in Flow Cytometry

Tue Nov 07 00:00:00 EST 2023

Prevention of EAE by tolerogenic vaccination with PEGylated antigenic peptides.

Therapeutic advances in chronic disease

Pfeil J,Simonetti M,Lauer U,von Thülen B,Durek P,Poulsen C,Pawlowska J,Kröger M,Krähmer R,Leenders F,Hoffmann U,Hamann A

Published figure using GM-CSF monoclonal antibody (Product # 12-7331-82) in Flow Cytometry

Tue Nov 07 00:00:00 EST 2023

Astrocyte-Derived Pleiotrophin Mitigates Late-Stage Autoimmune CNS Inflammation.

Frontiers in immunology

Linnerbauer M,Lößlein L,Farrenkopf D,Vandrey O,Tsaktanis T,Naumann U,Rothhammer V

Published figure using GM-CSF monoclonal antibody (Product # 12-7331-82) in Flow Cytometry

Mon Feb 14 00:00:00 EST 2022

GM-CSF production by non-classical monocytes controls antagonistic LPS-driven functions in allergic inflammation.

Cell reports

Kaur K,Bachus H,Lewis C,Papillion AM,Rosenberg AF,Ballesteros-Tato A,León B

12-7331-82 was used in Flow cytometry/Cell sorting to show here that lipopolysaccharide activity switches from pro-pathogenic to protective depending on the production of granulocyte-macrophage colony-stimulating factor by non-classical monocytes.

Tue Dec 28 00:00:00 EST 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

12-7331 was used in Flow cytometry/Cell sorting to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

Regulatory T Cells Restrain Interleukin-2- and Blimp-1-Dependent Acquisition of Cytotoxic Function by CD4⁺ T Cells.

Immunity

Śledzińska A,Vila de Mucha M,Bergerhoff K,Hotblack A,Demane DF,Ghorani E,Akarca AU,Marzolini MAV,Solomon I,Vargas FA,Pule M,Ono M,Seddon B,Kassiotis G,Ariyan CE,Korn T,Marafioti T,Lord GM,Stauss H,Jenner RG,Peggs KS,Quezada SA

12-7331 was used in Flow cytometry/Cell sorting to underscore the role of Treg cells, IL-2, and Blimp-1 in controlling the differentiation of cytotoxic CD4+ T cells and offer a pathway to enhancement of anti-tumor activity through their manipulation.

Tue Jan 14 00:00:00 EST 2020

Regulatory T Cells Restrain Interleukin-2- and Blimp-1-Dependent Acquisition of Cytotoxic Function by CD4⁺ T Cells.

Immunity

Tue Jan 14 00:00:00 EST 2020

Interleukin-17A Serves a Priming Role in Autoimmunity by Recruiting IL-1β-Producing Myeloid Cells that Promote Pathogenic T Cells.

Immunity

McGinley AM,Sutton CE,Edwards SC,Leane CM,DeCourcey J,Teijeiro A,Hamilton JA,Boon L,Djouder N,Mills KHG

12-7331 was used in Flow cytometry/Cell sorting to demonstrate that a key function of IL-17A in central nervous system (CNS) autoimmunity is to recruit IL-1β-secreting myeloid cells that prime pathogenic γδT17 and Th17 cells.

Tue Feb 18 00:00:00 EST 2020

Insulin-Like Growth Factors Are Key Regulators of T Helper 17 Regulatory T Cell Balance in Autoimmunity.

Immunity

DiToro D,Harbour SN,Bando JK,Benavides G,Witte S,Laufer VA,Moseley C,Singer JR,Frey B,Turner H,Bruning J,Darley-Usmar V,Gao M,Conover C,Hatton RD,Frank S,Colonna M,Weaver CT

12-7331 was used in Flow cytometry/Cell sorting to show that, among effector T cell subsets, Th17 and Treg cells selectively expressed multiple components of the IGF system.

Tue Apr 14 00:00:00 EDT 2020

Interleukin-1 promotes autoimmune neuroinflammation by suppressing endothelial heme oxygenase-1 at the blood-brain barrier.

Acta neuropathologica

Hauptmann J,Johann L,Marini F,Kitic M,Colombo E,Mufazalov IA,Krueger M,Karram K,Moos S,Wanke F,Kurschus FC,Klein M,Cardoso S,Strauß J,Bolisetty S,Lühder F,Schwaninger M,Binder H,Bechman I,Bopp T,Agarwal A,Soares MP,Regen T,Waisman A

12-7331-82 was used in Flow Cytometry to studied the role of IL-1 signaling in blood-brain barrier endothelial cells, astrocytes, and microglia for experimental autoimmune encephalomyelitis development.

Thu Oct 01 00:00:00 EDT 2020

Cross-talk between T Cells and Hematopoietic Stem Cells during Adoptive Cellular Therapy for Malignant Glioma.

Clinical cancer research : an official journal of the American Association for Cancer Research

Wildes TJ,Grippin A,Dyson KA,Wummer BM,Damiani DJ,Abraham RS,Flores CT,Mitchell DA

Published figure using GM-CSF monoclonal antibody (Product # 12-7331-82) in Flow Cytometry

Wed Aug 15 00:00:00 EDT 2018

Cholesterol Accumulation in Dendritic Cells Links the Inflammasome to Acquired Immunity.

Cell metabolism

Westerterp M,Gautier EL,Ganda A,Molusky MM,Wang W,Fotakis P,Wang N,Randolph GJ,D'Agati VD,Yvan-Charvet L,Tall AR

12-7331 was used in Flow cytometry/Cell sorting to identify an essential role of DC cholesterol efflux pathways in maintaining immune tolerance.

Tue Jun 06 00:00:00 EDT 2017

Bacillus anthracis lethal toxin negatively modulates ILC3 function through perturbation of IL-23-mediated MAPK signaling.

PLoS pathogens

Seshadri S,Allan DSJ,Carlyle JR,Zenewicz LA

12-7331-82 was used in Flow Cytometry to study the effects of MAPK signalling pathways in group 3 innate immune lymphocytes using Bacillus anthracis.

Sun Oct 01 00:00:00 EDT 2017

Protection from experimental autoimmune encephalomyelitis by polyclonal IgG requires adjuvant-induced inflammation.

Journal of neuroinflammation

Quast I,Keller CW,Weber P,Schneider C,von Gunten S,Lünemann JD

12-7331 was used in Flow cytometry/Cell sorting to identify factors affecting the efficacy of intravenous immunoglobulin treatment for multiple sclerosis.

Thu Feb 18 00:00:00 EST 2016

B cell-derived transforming growth factor-β1 expression limits the induction phase of autoimmune neuroinflammation.

Scientific reports

Bjarnadóttir K,Benkhoucha M,Merkler D,Weber MS,Payne NL,Bernard CCA,Molnarfi N,Lalive PH

12-7331-82 was used in Flow Cytometry to test the role for transforming growth factor-β1 expression in regulatory B cell function in autoimmune neuroinflammation.

Thu Oct 06 00:00:00 EDT 2016

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals.

Nature immunology

Salmond RJ,Brownlie RJ,Morrison VL,Zamoyska R

12-7331 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity.

Mon Sep 01 00:00:00 EDT 2014

Site-specific chemokine expression regulates central nervous system inflammation and determines clinical phenotype in autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Stoolman JS,Duncker PC,Huber AK,Segal BM

12-7331 was used in Flow cytometry/Cell sorting to investigate the role of CXCL2 in atypical EAE, showing that site-specific chemokine expression regulates CNS inflammation and determines clinical phenotype.

Tue Jul 15 00:00:00 EDT 2014

Phenotypic Identification of Spinal Cord-Infiltrating CD4⁺ T Lymphocytes in a Murine Model of Neuropathic Pain.

Journal of pain & relief

Draleau K,Maddula S,Slaiby A,Nutile-McMenemy N,De Leo J,Cao L

12-7331 was used in Flow cytometry/Cell sorting to investigate the phenotype of spinal cord-infiltrating CD4+ T lymphocytes involved in the maintenance of neuropathic pain in a murine model.

Sun Jun 01 00:00:00 EDT 2014

Intravenous gammaglobulin inhibits encephalitogenic potential of pathogenic T cells and interferes with their trafficking to the central nervous system, implicating sphingosine-1 phosphate receptor 1-mammalian target of rapamycin axis.

Journal of immunology (Baltimore, Md. : 1950)

Othy S,Hegde P,Topçu S,Sharma M,Maddur MS,Lacroix-Desmazes S,Bayry J,Kaveri SV

12-7331 was used in Flow cytometry/Cell sorting to reveal a novel role of Igs in the modulation of polarisation and trafficking of T lymphocytes, accounting for the observed beneficial effect in IVIg therapy.

Wed May 01 00:00:00 EDT 2013

Autoreactive Tbet-positive CD4 T cells develop independent of classic Th1 cytokine signaling during experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Yeh WI,McWilliams IL,Harrington LE

12-7331 was used in Flow cytometry/Cell sorting to study changes in expression of Tbet by CD4 T cells from distinct areas of the body following the onset of experimental autoimmune encephalomyelitis.

Tue Nov 15 00:00:00 EST 2011

Bioinformatics

Protein Aliases: Colony-stimulating factor; CSF; GM-CSF; granulocyte macrophage colony stimulating factor; granulocyte-macrophage colony stimulating factor 2; Granulocyte-macrophage colony-stimulating factor; M-GM-CSF; MGC131935; MGC138897; MGC151255; MGC151257; put. GM-CSF

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Gene Aliases: Csf2; Csfgm; Gm-CSf; GMCSF; MGI-IGM

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UniProt ID: (Mouse) P01587

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Entrez Gene ID: (Mouse) 12981

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Function(s)

cytokine activity granulocyte macrophage colony-stimulating factor receptor binding growth factor activity cytokine

Process(es)

embryonic placenta development immune response positive regulation of cell proliferation regulation of gene expression positive regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of interleukin-23 production epithelial fluid transport macrophage activation regulation of cell proliferation positive regulation of tyrosine phosphorylation of Stat5 protein myeloid dendritic cell differentiation positive regulation of DNA replication negative regulation of cytolysis positive regulation of podosome assembly dendritic cell differentiation negative regulation of extrinsic apoptotic signaling pathway in absence of ligand

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GM-CSF Monoclonal Antibody (MP1-22E9), PE, eBioscience™

GM-CSF Antibody (12-7331-82) in Flow

Product Details

12-7331-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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