Invitrogen

Gata-3 Monoclonal Antibody (TWAJ), PE-Cyanine7, eBioscience™

Gata-3 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 21

Gata-3 Antibody (25-9966-42) in Flow

C57Bl/6 thymocytes were surface stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and Anti-Mouse CD8a APC (Product # 17-0081-82) followed by intracellular staining with Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82) (blue histogram) or Anti-Human/Mouse Gata-3 PE-Cyanine7 (purple histogram) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the CD4 single positive (left) or CD8 single positive (right) gates were used for analysis.

Product Image Gallery

Product Details

25-9966-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.00375 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rhesus monkey

Published species

Human, Mouse, Non-human primate

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TWAJ

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573568

Product Specific Information

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(Product # 00-5523-00)

Crossreactivity in rhesus monkeys has been published.

Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.00375 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

25-9966-42 was used in Flow cytometry/Cell sorting to report that human blood CD1c+ dendritic cells activated by GM-CSF (also known as CSF2) drive the differentiation of naive CD4+ T cells into Tfh1 cells.

Tue Nov 01 00:00:00 EDT 2022

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Tue Nov 01 00:00:00 EDT 2022

Airway Administration of Bacterial Lysate OM-85 Protects Mice Against Respiratory Syncytial Virus Infection.

Frontiers in immunology

Antunes KH,Cassão G,Santos LD,Borges SG,Poppe J,Gonçalves JB,Nunes EDS,Recacho GF,Sousa VB,Da Silva GS,Mansur D,Stein RT,Pasquali C,De Souza APD

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Tue May 24 00:00:00 EDT 2022

The molecular and epigenetic mechanisms of innate lymphoid cell (ILC) memory and its relevance for asthma.

The Journal of experimental medicine

Verma M,Michalec L,Sripada A,McKay J,Sirohi K,Verma D,Sheth D,Martin R,Dyjack N,Seibold MA,Knapp JR,Tu TH,O'Connor BP,Gorska MM,Alam R

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Thu Dec 02 00:00:00 EST 2021

Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma.

Cell reports. Medicine

Chao JL,Korzinkin M,Zhavoronkov A,Ozerov IV,Walker MT,Higgins K,Lingen MW,Izumchenko E,Savage PA

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

LmjF.36.3850, a novel hypothetical Leishmania major protein, contributes to the infection.

Immunology

Zutshi S,Sarode AY,Ghosh SK,Jha MK,Sudan R,Kumar S,Sadhale LP,Roy S,Saha B

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

25-9966-42 was used in Flow cytometry/Cell sorting to suggest that graded RhoA expression in Treg cells distinguishes tumor immunity from autoimmunity and that rational targeting of RhoA in Treg cells may trigger anti-tumor T cell immunity without causing autoimmune responses.

Tue Jan 11 00:00:00 EST 2022

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Tue Jan 11 00:00:00 EST 2022

BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

Nature

Vogel AB,Kanevsky I,Che Y,Swanson KA,Muik A,Vormehr M,Kranz LM,Walzer KC,Hein S,Güler A,Loschko J,Maddur MS,Ota-Setlik A,Tompkins K,Cole J,Lui BG,Ziegenhals T,Plaschke A,Eisel D,Dany SC,Fesser S,Erbar S,Bates F,Schneider D,Jesionek B,Sänger B,Wallisch AK,Feuchter Y,Junginger H,Krumm SA,Heinen AP,Adams-Quack P,Schlereth J,Schille S,Kröner C,de la Caridad Güimil Garcia R,Hiller T,Fischer L,Sellers RS,Choudhary S,Gonzalez O,Vascotto F,Gutman MR,Fontenot JA,Hall-Ursone S,Brasky K,Griffor MC,Han S,Su AAH,Lees JA,Nedoma NL,Mashalidis EH,Sahasrabudhe PV,Tan CY,Pavliakova D,Singh G,Fontes-Garfias C,Pride M,Scully IL,Ciolino T,Obregon J,Gazi M,Carrion R,Alfson KJ,Kalina WV,Kaushal D,Shi PY,Klamp T,Rosenbaum C,Kuhn AN,Türeci Ö,Dormitzer PR,Jansen KU,Sahin U

25-9966-42 was used in flow cytometry to report the preclinical development of two vaccine candidates (BNT162b1 and BNT162b2) that contain nucleoside-modified messenger RNA that encodes immunogens derived from the spike glycoprotein (S) of SARS-CoV-2, formulated in lipid nanoparticles.

Thu Apr 01 00:00:00 EDT 2021

BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

Nature

Thu Apr 01 00:00:00 EDT 2021

BNT162b vaccines protect rhesus macaques from SARS-CoV-2.

Nature

Thu Apr 01 00:00:00 EDT 2021

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

The obesity-induced adipokine sST2 exacerbates adipose T_reg and ILC2 depletion and promotes insulin resistance.

Science advances

Zhao XY,Zhou L,Chen Z,Ji Y,Peng X,Qi L,Li S,Lin JD

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Fri May 01 00:00:00 EDT 2020

B cells induced by Schistosoma japonicum infection display diverse regulatory phenotypes and modulate CD4⁺ T cell response.

Parasites & vectors

Xiao J,Guan F,Sun L,Zhang Y,Zhang X,Lu S,Liu W

25-9966-42 was used in Flow Cytometry to show that Schistosoma japonicum infection modulates the differentiation of B cell subsets that have the capability to affect the CD4+ T cell response.

Fri Mar 20 00:00:00 EDT 2020

Compartmentalized gut lymph node drainage dictates adaptive immune responses.

Nature

Esterházy D,Canesso MCC,Mesin L,Muller PA,de Castro TBR,Lockhart A,ElJalby M,Faria AMC,Mucida D

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

TREM-1-dependent M1 macrophage polarization restores intestinal epithelium damaged by DSS-induced colitis by activating IL-22-producing innate lymphoid cells.

Journal of biomedical science

Yang FC,Chiu PY,Chen Y,Mak TW,Chen NJ

Wed Jun 12 00:00:00 EDT 2019

IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization.

Nature communications

Eissmann MF,Dijkstra C,Jarnicki A,Phesse T,Brunnberg J,Poh AR,Etemadi N,Tsantikos E,Thiem S,Huntington ND,Hibbs ML,Boussioutas A,Grimbaldeston MA,Buchert M,O'Donoghue RJJ,Masson F,Ernst M

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Fri Jun 21 00:00:00 EDT 2019

Establishment of Chronic Typhoid Infection in a Mouse Carriage Model Involves a Type 2 Immune Shift and T and B Cell Recruitment to the Gallbladder.

mBio

González JF,Kurtz J,Bauer DL,Hitt R,Fitch J,Wetzel A,La Perle K,White P,McLachlan J,Gunn JS

25-9966 was used in Flow cytometry/Cell sorting to analyze the transcriptional profile of the gallbladder in a mouse model of chronic carriage.

Tue Oct 01 00:00:00 EDT 2019

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

25-9966 was used in Flow cytometry/Cell sorting to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 EDT 2017

ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control.

The Journal of experimental medicine

Schwartz C,Khan AR,Floudas A,Saunders SP,Hams E,Rodewald HR,McKenzie ANJ,Fallon PG

25-9966 was used in Flow cytometry/Cell sorting to study how group 2 innate lymphoid cells regulate adaptive Th2 cell functions.

Mon Sep 04 00:00:00 EDT 2017

Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Wellcome open research

Dutton EE,Camelo A,Sleeman M,Herbst R,Carlesso G,Belz GT,Withers DR

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Sat Jan 09 00:00:00 EST 2021

Antibiotic-induced perturbations in microbial diversity during post-natal development alters amyloid pathology in an aged APP_SWE/PS1_ΔE9 murine model of Alzheimer's disease.

Scientific reports

Minter MR,Hinterleitner R,Meisel M,Zhang C,Leone V,Zhang X,Oyler-Castrillo P,Zhang X,Musch MW,Shen X,Jabri B,Chang EB,Tanzi RE,Sisodia SS

25-9966 was used in Flow cytometry/Cell sorting to investigate how ABX-induced microbial diversity perturbations during post-natal stages of development coincide with altered host immunity mechanisms and amyloidosis in a murine model of AD.

Tue Sep 05 00:00:00 EDT 2017

Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3.

PloS one

Deng YQ,Yang YQ,Wang SB,Li F,Liu MZ,Hua QQ,Tao ZZ

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Tue May 31 00:00:00 EDT 2016

Impaired proteasome function activates GATA3 in T cells and upregulates CTLA-4: relevance for Sézary syndrome.

The Journal of investigative dermatology

Gibson HM,Mishra A,Chan DV,Hake TS,Porcu P,Wong HK

Published figure using Gata-3 monoclonal antibody (Product # 25-9966-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Role of CD44 in the differentiation of Th1 and Th2 cells: CD44-deficiency enhances the development of Th2 effectors in response to sheep RBC and chicken ovalbumin.

Journal of immunology (Baltimore, Md. : 1950)

Guan H,Nagarkatti PS,Nagarkatti M

Wed Jul 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: GATA binding factor-3; GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

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Gene Aliases: EGK_19445; Gata-3; GATA3; HDR; HDRS; jal

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UniProt ID: (Human) P23771, (Mouse) P23772

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Entrez Gene ID: (Pig) 733631, (Rhesus monkey) 713840, (Human) 2625, (Mouse) 14462

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding sequence-specific DNA binding metal ion binding DNA-binding transcription factor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Gata-3 Monoclonal Antibody (TWAJ), PE-Cyanine7, eBioscience™

Gata-3 Antibody (25-9966-42) in Flow

Product Details

25-9966-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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