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Invitrogen
Granulysin Monoclonal Antibody (eBioDH2 (DH2)), Alexa Fluor™ 647, eBioscience™
FIGURE: 1 / 5
Granulysin Antibody (51-8828-42) in Flow
Normal human peripheral blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD56 Monoclonal Antibody, FITC (Product # 11-0566-42) and Mouse IgG1 kappa Isotype Control, Alexa Fluor 647 (Product # 51-4714-81) (left) or Granulysin Monoclonal Antibody, Alexa Fluor 647 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
51-8828-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
eBioDH2 (DH2)
Conjugate
Excitation/Emission Max
650/671 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815200
Product Specific Information
Description: The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin is a member of the saposin-like protein (SAPLIP) family. Two proteins 9 and 15kDa are thought to be post translation modified versions of the 15kDa protein with a change in confirmation. Granulysin is found in NK and activated T cells and the expression is confined to the cytolytic granules which also contains perforin and granzyme. Granulysin with perforn are suspected to function synergistically to kill bacteria and induce apoptosis in nucleated cells.
Applications Reported: This eBioDh2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This eBioDh2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.
Target Information
GNLY is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. GNLY is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: Granulysin; lymphocyte-activation gene 2; Lymphokine; Lymphokine LAG-2; Lymphokine NKG5519; Protein NKG5; T-cell activation protein 519; T-lymphocyte activation gene 519
Gene Aliases: D2S69E; GNLY; LAG-2; LAG2; NKG5; TLA519
UniProt ID: (Human) P22749
Entrez Gene ID: (Human) 10578
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