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Invitrogen
Granzyme B Monoclonal Antibody (GB11), PE-Cyanine5.5
FIGURE: 1 / 1
Granzyme B Antibody (GRB18) in Flow
Science translational medicine 2022 -
Figure 6. Upregulation of exhaustion-related transcription factors in PRDM1 KO CAR T-cells is attributed to increased chromatin accessibility and calcineurin-NFAT signaling. ( A to D ) ATAC-seq analysis of AAVS1 KO and PRDM1 KO CAR T-cells is shown. At the end of manufacturing, CAR T-cells were enriched and subjected to ATAC-seq analysis. ( A ) The volcano plot shows differentially accessible chromatin reg... View More
Product Details
GRB18
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
GB11
Immunogen
Human Granzyme B.
Conjugate
Excitation/Emission Max
564/692 nm
View spectra
Form
Liquid
Purification
purified
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536541
Target Information
Granzyme B is a member of the granzyme serine protease family, and is found in the granules of cytotoxic T cells and NK cells. Granzyme B has been described as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation, and CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. Granzyme B is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved in this process has been identified as mannose 6-phosphate receptor which functions as a death receptor for Granzyme B during cytotoxic T cell-induced apoptosis. Granzyme B enters target cells to cleave caspase-3 and initiate the caspase cascade leading to DNA fragmentation and apoptosis. Granzyme B can also act through a mitochondrial apoptosis pathway by cleaving the Bid protein. Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes (A to G) have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C11; Cathepsin G-like 1; CTLA-1; CTSGL1; cytotoxic serine protease B; Cytotoxic T lymphocyte associated serine esterase 1; Cytotoxic T-lymphocyte proteinase 2; cytotoxic T-lymphocyte-associated serine esterase 1; fragmentin 2; Fragmentin-2; granzyme 2; Granzyme B; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); Granzyme-2; GranzymeB; HLP; Human lymphocyte protein; Human lymphocyte protein (Hlp); Lymphocyte protease; OTTHUMP00000028189; SECT; T-cell serine protease 1-3E
Gene Aliases: CCPI; CGL-1; CGL1; CSP-B; CSPB; CTLA1; CTSGL1; GRB; GZMB; HLP; SECT
UniProt ID: (Human) P10144
Entrez Gene ID: (Human) 3002
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