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Invitrogen
HLA-DR Monoclonal Antibody (LN3), NovaFluor™ Blue 610-30S, eBioscience™
FIGURE: 1 / 3
HLA-DR Antibody (H049T03B05-A) in Flow
Normal human peripheral blood cells were unstained (left) or stained with HLA-DR Monoclonal Antibody, NovaFluor Blue 610-30S (Product #: h049t03b05) (right). All cells were co-stained with CD19 Monoclonal Antibody, eFluor 450 (Product #: 48-0199-42). Total viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product #: L34962). Data was acquired on a 5-laser Cytek Aurora.
Product Details
H049T03B05-A
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b, kappa
Class
Monoclonal
Type
Antibody
Clone
LN3
Conjugate
Excitation/Emission Max
508/615 nm
View spectra
Form
Liquid
Concentration
0.8 µg/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_3098592 (AB_2925977)
Product Specific Information
Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4^+ T lymphocytes.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: The LN3 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.0075 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 509 nm; Emission: 614 nm; Laser: 488 nm (Blue) Laser
Target Information
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DASS-397D15.1; DR beta-5; DR-9; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; leukocyte antigen; leukocyte antigen class II; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen HLA-DR-beta; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; p33; XXbac-BPG161M6.1
Gene Aliases: CD74; DHLAG; DR-4; DR4; DRB1; DRB4; DRw10; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; Ia-GAMMA; II; MLRW; SS1
UniProt ID: (Human) P04233, (Human) P01903, (Human) Q5UBA2, (Human) P79483, (Human) P13762, (Human) Q30154
Entrez Gene ID: (Human) 972, (Human) 3122, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127
beta-amyloid binding
cytokine receptor activity
protein binding
cytokine binding
MHC class II protein complex binding
macrophage migration inhibitory factor binding
MHC class II protein binding
MHC class II protein binding, via antigen binding groove
identical protein binding
protein binding involved in protein folding
nitric-oxide synthase binding
MHC class II receptor activity
peptide antigen binding
major histocompatibility complex protein
scaffold/adaptor protein
activation of MAPK activity
prostaglandin biosynthetic process
positive regulation of cytokine-mediated signaling pathway
positive regulation of dendritic cell antigen processing and presentation
negative regulation of peptide secretion
positive regulation of type 2 immune response
negative regulation of mature B cell apoptotic process
protein complex assembly
intracellular protein transport
defense response
signal transduction
cell proliferation
immunoglobulin mediated immune response
antigen processing and presentation of endogenous antigen
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of B cell proliferation
macrophage migration inhibitory factor signaling pathway
regulation of macrophage activation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
T cell selection
positive thymic T cell selection
negative thymic T cell selection
negative regulation of T cell differentiation
positive regulation of T cell differentiation
positive regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
chaperone mediated protein folding requiring cofactor
positive regulation of macrophage cytokine production
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
peptide antigen assembly with MHC class II protein complex
antigen processing and presentation of peptide or polysaccharide antigen via MHC class II
polysaccharide assembly with MHC class II protein complex
immune response
viral process
T cell costimulation
T cell receptor signaling pathway
cognition
interferon-gamma-mediated signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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