Invitrogen

HA Tag Monoclonal Antibody (2-2.2.14), Alexa Fluor™ 647

HA Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 3

HA Tag Antibody (26183-A647) in ICC/IF

Immunofluorescent analysis of HA Tag was performed using 70% confluent HA-H3 transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Anti-HA Tag Monoclonal Antibody (2-2.2.14), Alexa Fluor 647 (Product # 26183-A647) at 1:200 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labe... View More

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Product Details

26183-A647

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

- -

Immunocytochemistry (ICC/IF)

1:200

View 3 publications 3 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2-2.2.14

Immunogen

human influenza hemagglutinin, HA-tag, HA, HA Epitope Tag

Conjugate

Alexa Fluor™ 647 Alexa Fluor™ 647 Alexa Fluor™ 647

Excitation/Emission Max

650/671 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610626

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Neurexin-3 subsynaptic densities are spatially distinct from Neurexin-1 and essential for excitatory synapse nanoscale organization in the hippocampus.

Nature communications

Lloyd BA,Han Y,Roth R,Zhang B,Aoto J

26183-A647 was used in Immunocytochemistry-immunofluorescence to identify neurexin-3 as a critical presynaptic adhesion molecule that regulates excitatory synapse nano-organization in hippocampus.

Sat Aug 05 00:00:00 EDT 2023

SNX5 targets a monoamine transporter to the TGN for assembly into dense core vesicles by AP-3.

The Journal of cell biology

Xu H,Chang F,Jain S,Heller BA,Han X,Liu Y,Edwards RH

26183-A647 was used in Immunocytochemistry to identify a transient role for AP-3 at the TGN, where it associates with DCV cargo.

Mon May 02 00:00:00 EDT 2022

Rapid generation of potent antibodies by autonomous hypermutation in yeast.

Nature chemical biology

Wellner A,McMahon C,Gilman MSA,Clements JR,Clark S,Nguyen KM,Ho MH,Hu VJ,Shin JE,Feldman J,Hauser BM,Caradonna TM,Wingler LM,Schmidt AG,Marks DS,Abraham J,Kruse AC,Liu CC

26183-A647 was used in Flow cytometry/Cell sorting to develop the autonomous hypermutation yeast surface display technique for recombinant antibody generation.

Fri Oct 01 00:00:00 EDT 2021

Genetic Code Expansion and Click-Chemistry Labeling to Visualize GABA-A Receptors by Super-Resolution Microscopy.

Frontiers in synaptic neuroscience

Kuhlemann A,Beliu G,Janzen D,Petrini EM,Taban D,Helmerich DA,Doose S,Bruno M,Barberis A,Villmann C,Sauer M,Werner C

26183-A647 was used in Immunocytochemistry, Western Blot, Sample Prep to report site-specific labeling of the extracellular domain of γ-aminobutyric acid type A (GABA-A) receptor subunits by genetic code expansion (GCE) with unnatural amino acids (ncAA) combined with bioorthogonal click-chemistry labeling with tetrazine dyes in HEK-293-T cells and primary cultured neurons.

Wed Dec 15 00:00:00 EST 2021

Molecular basis for the evolved instability of a human G-protein coupled receptor.

Cell reports

Chamness LM,Zelt NB,Harrington HR,Kuntz CP,Bender BJ,Penn WD,Ziarek JJ,Meiler J,Schlebach JP

26183-A647 was used in Flow cytometry/Cell sorting to suggest that the marginal energetics of cotranslational folding can be exploited to tune membrane protein fitness.

Tue Nov 23 00:00:00 EST 2021

A toolbox of nanobodies developed and validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons.

eLife

Dong JX,Lee Y,Kirmiz M,Palacio S,Dumitras C,Moreno CM,Sando R,Santana LF,Südhof TC,Gong B,Murray KD,Trimmer JS

26183-A647 was used in Immunocytochemistry-immunofluorescence to report the generation and validation of nAbs against a set of proteins prominently expressed at specific subcellular sites in mammalian brain neurons.

Mon Sep 30 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin

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