Invitrogen

HA Tag Monoclonal Antibody (2-2.2.14), DyLight™ 550

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

8 References

View all (114) HA Tag antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

HA Tag Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

HA Tag Antibody (26183-D550) in ICC/IF

Immunofluorescent analysis of HA Tag was performed using 70% confluent HA-H3 transfected HEK-293E cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with HA Tag Monoclonal Antibody (2-2.2.14), DyLight 550 (Product # 26183-D550) at 1:200 dilution and Histone H3 Rabbit Polyclonal Antibody (Product # 711055) at 0.5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with ... View More

HA Tag Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

26183-D550

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:25-1:200

View 2 publications 2 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2-2.2.14

Immunogen

HA peptide YPYDVPDYA derivitized to ovalbumin.

Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

Excitation/Emission Max

556/570 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533052

Product Specific Information

26183-D550 has been successfully used for immunofluorescence. DyLight 550 has an excitation/emission of 562/576 nm.

HA Synthetic Peptide (Product # 26184) is available for use in neutralization and control experiments and to competitively elute HA-tagged fusion proteins from immobilized anti-HA affinity resin.

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Lysine acetylation regulates moonlighting activity of the E2 subunit of the chloroplast pyruvate dehydrogenase complex in Chlamydomonas.

The Plant journal : for cell and molecular biology

Neusius D,Kleinknecht L,Teh JT,Ostermeier M,Kelterborn S,Eirich J,Hegemann P,Finkemeier I,Bohne AV,Nickelsen J

26183-D550 was used in Immunocytochemistry-immunofluorescence to report on the characterization of the molecular switch that regulates shuttling of DLA2 between its functions in carbon metabolism and D1 synthesis.

Thu Sep 01 00:00:00 EDT 2022

Molecular basis for the evolved instability of a human G-protein coupled receptor.

Cell reports

Chamness LM,Zelt NB,Harrington HR,Kuntz CP,Bender BJ,Penn WD,Ziarek JJ,Meiler J,Schlebach JP

26183-D550 was used in Flow cytometry/Cell sorting to suggest that the marginal energetics of cotranslational folding can be exploited to tune membrane protein fitness.

Tue Nov 23 00:00:00 EST 2021

Tissue topography steers migrating Drosophila border cells.

Science (New York, N.Y.)

Dai W,Guo X,Cao Y,Mondo JA,Campanale JP,Montell BJ,Burrous H,Streichan S,Gov N,Rappel WJ,Montell DJ

26183-D550 was used in Immunohistochemistry-immunofluorescence to investigate how physical features of the microenvironment influence path selection of Drosophila border cells.

Fri Nov 20 00:00:00 EST 2020

Highly Conserved Molecular Features in IgLONs Contrast Their Distinct Structural and Biological Outcomes.

Journal of molecular biology

Venkannagari H,Kasper JM,Misra A,Rush SA,Fan S,Lee H,Sun H,Seshadrinathan S,Machius M,Hommel JD,Rudenko G

26183-D550 was used in Immunohistochemistry (PFA) to reveal homodimeric structures of Neuronal growth regulator 1 and neurotrimin.

Fri Sep 04 00:00:00 EDT 2020

Conserved nuclear hormone receptors controlling a novel plastic trait target fast-evolving genes expressed in a single cell.

PLoS genetics

Sieriebriennikov B,Sun S,Lightfoot JW,Witte H,Moreno E,Rödelsperger C,Sommer RJ

26183-D550 was used in Immunohistochemistry-immunofluorescence to investigate the gene regulatory network controlling predatory vs. non-predatory dimorphism in the nematode Pristionchus pacificus.

Wed Apr 01 00:00:00 EDT 2020

Aggregate Filamentous Growth Responses in Yeast.

mSphere

Chow J,Dionne HM,Prabhakar A,Mehrotra A,Somboonthum J,Gonzalez B,Edgerton M,Cullen PJ

26183-D550 was used in Immunocytochemistry-immunoflourescence to identify responses in Saccharomyces cerevisiae and the opportunistic pathogen Candida albicans where cells undergo filamentous growth in groups of cells or aggregates.

Wed Mar 06 00:00:00 EST 2019

The Retinitis Pigmentosa-Linked Mutations in Transmembrane Helix 5 of Rhodopsin Disrupt Cellular Trafficking Regardless of Oligomerization State.

Biochemistry

Mallory DP,Gutierrez E,Pinkevitch M,Klinginsmith C,Comar WD,Roushar FJ,Schlebach JP,Smith AW,Jastrzebska B

26183-D550 was used in Flow Cytometry to suggest that the RP pathology associated with the expression of the V209M and F220C mutants could arise from defects in folding and cellular trafficking rather than the disruption of dimerization.

Tue Sep 04 00:00:00 EDT 2018

Valproic acid causes proteasomal degradation of DICER and influences miRNA expression.

PloS one

Zhang Z,Convertini P,Shen M,Xu X,Lemoine F,de la Grange P,Andres DA,Stamm S

26183-D550 was used in western blot to study the proteasomal degradation of DICER and altered miRNA levels induced by valproic acid

Tue Oct 21 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin

View more View less

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

HA Tag Monoclonal Antibody (2-2.2.14), DyLight™ 550

HA Tag Antibody (26183-D550) in ICC/IF

Product Details

26183-D550

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!