Invitrogen

HA Tag Monoclonal Antibody (2-2.2.14), HRP

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

8 Published Figures

14 References

View all (114) HA Tag antibodies

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HA Tag Antibody (26183-HRP) in WB

Western blot was performed using HRP-conjugated Anti-HA Mouse Monoclonal Antibody (Product # 26183-HRP) by loading whole cell extracts of untransfected and transiently transfected HEK-293E lysates: untransfected, 40 µg (Lane 1), empty vector control, 40 µg (Lane 2), HA-H3, 40 µg (Lane 3), HA-H3, 20 µg (Lane 4), HA-H3, 10 µg (Lane 5), HA-H3, 5 µg (Lane 6), p65-HA, 40 µg (Lane 7), Myc-p65-V5, 40 µg (Lane 8) and 25 ng of Positope (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins we... View More

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Product Details

26183-HRP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 14 publications 14 publications

Product Specifications

Species Reactivity

Tag

Published species

Tag

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2-2.2.14

Immunogen

HA peptide YPYDVPDYA derivitized to ovalbumin.

Conjugate

HRP HRP HRP

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

proprietary buffer, pH 7.4

Contains

0.07% Kathon

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2533056

Target Information

HA Tag (hemagglutinin tag) is an epitope tag that provides a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HA (hemagglutinin) is a surface glycoprotein derived from influenza hemagglutinin, and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. HA tag antibodies can be used to facilitate protein detection or co-immunopreciptation of tagged proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

TRIM28-dependent SUMOylation protects the adult ovary from activation of the testicular pathway.

Nature communications

Rossitto M,Déjardin S,Rands CM,Le Gras S,Migale R,Rafiee MR,Neirijnck Y,Pruvost A,Nguyen AL,Bossis G,Cammas F,Le Gallic L,Wilhelm D,Lovell-Badge R,Boizet-Bonhoure B,Nef S,Poulat F

26183-HRP was used in Western Blotting to show that TRIM28 is required to prevent female-to-male sex reversal of the mouse ovary after birth.

Fri Jul 29 00:00:00 EDT 2022

TMK-based cell-surface auxin signalling activates cell-wall acidification.

Nature

Lin W,Zhou X,Tang W,Takahashi K,Pan X,Dai J,Ren H,Zhu X,Pan S,Zheng H,Gray WM,Xu T,Kinoshita T,Yang Z

Published figure using HA Tag monoclonal antibody (Product # 26183-HRP) in Western Blot

Mon Nov 01 00:00:00 EDT 2021

Modified chromosome structure caused by phosphomimetic H2A modulates the DNA damage response by increasing chromatin mobility in yeast.

Journal of cell science

García Fernández F,Lemos B,Khalil Y,Batrin R,Haber JE,Fabre E

26183-HRP was used in Western Blot to investigate the role of H2A phosphorylation in the DNA damage response.

Mon Mar 29 00:00:00 EDT 2021

The N-terminus of an Ustilaginoidea virens Ser-Thr-rich glycosylphosphatidylinositol-anchored protein elicits plant immunity as a MAMP.

Nature communications

Song T,Zhang Y,Zhang Q,Zhang X,Shen D,Yu J,Yu M,Pan X,Cao H,Yong M,Qi Z,Du Y,Zhang R,Yin X,Qiao J,Liu Y,Liu W,Sun W,Zhang Z,Wang Y,Dou D,Ma Z,Liu Y

26183-HRP was used in Western Blotting to show that a microbe-associated molecular pattern (MAMP), Ser-Thr-rich Glycosyl-phosphatidyl-inositol-anchored protein (SGP1) from U. virens, induces immune responses in rice leaves but not panicles.

Tue Apr 27 00:00:00 EDT 2021

The N-terminus of an Ustilaginoidea virens Ser-Thr-rich glycosylphosphatidylinositol-anchored protein elicits plant immunity as a MAMP.

Nature communications

Song T,Zhang Y,Zhang Q,Zhang X,Shen D,Yu J,Yu M,Pan X,Cao H,Yong M,Qi Z,Du Y,Zhang R,Yin X,Qiao J,Liu Y,Liu W,Sun W,Zhang Z,Wang Y,Dou D,Ma Z,Liu Y

Tue Apr 27 00:00:00 EDT 2021

Molecular basis of the interaction of the human tyrosine phosphatase PTPN3 with the hepatitis B virus core protein.

Scientific reports

Genera M,Quioc-Salomon B,Nourisson A,Colcombet-Cazenave B,Haouz A,Mechaly A,Matondo M,Duchateau M,König A,Windisch MP,Neuveut C,Wolff N,Caillet-Saguy C

Published figure using HA Tag monoclonal antibody (Product # 26183-HRP) in Western Blot

Wed Jan 13 00:00:00 EST 2021

Molecular basis of the interaction of the human tyrosine phosphatase PTPN3 with the hepatitis B virus core protein.

Scientific reports

Genera M,Quioc-Salomon B,Nourisson A,Colcombet-Cazenave B,Haouz A,Mechaly A,Matondo M,Duchateau M,König A,Windisch MP,Neuveut C,Wolff N,Caillet-Saguy C

Published figure using HA Tag monoclonal antibody (Product # 26183-HRP) in Western Blot

Wed Jan 13 00:00:00 EST 2021

The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity.

PloS one

Lee J,Oh S,Bhattacharya S,Zhang Y,Florens L,Washburn MP,Workman JL

26183-HRP was used in Western Blot to indicate that the structure of PDC may not always maintain its ~ 10 MDa organization, but is rather variable.

Wed Jan 27 00:00:00 EST 2021

The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity.

PloS one

Lee J,Oh S,Bhattacharya S,Zhang Y,Florens L,Washburn MP,Workman JL

26183-HRP was used in Western Blot to indicate that the structure of PDC may not always maintain its ~ 10 MDa organization, but is rather variable.

Wed Jan 27 00:00:00 EST 2021

The rice NLR pair Pikp-1/Pikp-2 initiates cell death through receptor cooperation rather than negative regulation.

PloS one

Zdrzałek R,Kamoun S,Terauchi R,Saitoh H,Banfield MJ

Published figure using HA Tag monoclonal antibody (Product # 26183-HRP) in Western Blot

Wed Oct 28 00:00:00 EDT 2020

An RNA Repair Operon Regulated by Damaged tRNAs.

Cell reports

Hughes KJ,Chen X,Burroughs AM,Aravind L,Wolin SL

Published figure using HA Tag monoclonal antibody (Product # 26183-HRP) in Western Blot

Tue Dec 22 00:00:00 EST 2020

FGF23 expression is stimulated in transgenic α-Klotho longevity mouse model.

JCI insight

Xiao Z,King G,Mancarella S,Munkhsaikhan U,Cao L,Cai C,Quarles LD

Published figure using HA Tag monoclonal antibody (Product # 26183-HRP) in Western Blot

Thu Dec 05 00:00:00 EST 2019

Single-Domain Antibodies Represent Novel Alternatives to Monoclonal Antibodies as Targeting Agents against the Human Papillomavirus 16 E6 Protein.

International journal of molecular sciences

Togtema M,Hussack G,Dayer G,Teghtmeyer MR,Raphael S,Tanha J,Zehbe I

26183-HRP was used in Western Blotting to lead the way for subsequent studies into the ability of these novel molecules to inhibit HPV16-infected cells in vitro and in vivo.

Sun Apr 28 00:00:00 EDT 2019

Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched.

Cell

Zhang Y,Bulkley DP,Xin Y,Roberts KJ,Asarnow DE,Sharma A,Myers BR,Cho W,Cheng Y,Beachy PA

26183-HRP was used in Western Blotting to determine how the Patched 12-transmembrane protein (a Hedgehog receptor) is involved in cholesterol transport.

Thu Nov 15 00:00:00 EST 2018

Blocking c-Met-mediated PARP1 phosphorylation enhances anti-tumor effects of PARP inhibitors.

Nature medicine

Du Y,Yamaguchi H,Wei Y,Hsu JL,Wang HL,Hsu YH,Lin WC,Yu WH,Leonard PG,Lee GR,Chen MK,Nakai K,Hsu MC,Chen CT,Sun Y,Wu Y,Chang WC,Huang WC,Liu CL,Chang YC,Chen CH,Park M,Jones P,Hortobagyi GN,Hung MC

26183-HRP was used in Western blot to study the impact of blocking c-Met-mediated PARP1 phosphorylation on the anti-tumour effects of PARP1 inhibitors.

Mon Feb 01 00:00:00 EST 2016

Valproic acid causes proteasomal degradation of DICER and influences miRNA expression.

PloS one

Zhang Z,Convertini P,Shen M,Xu X,Lemoine F,de la Grange P,Andres DA,Stamm S

26183-HRP was used in western blot to study the proteasomal degradation of DICER and altered miRNA levels induced by valproic acid

Tue Oct 21 00:00:00 EDT 2014

Functional importance of the anaphase-promoting complex-Cdh1-mediated degradation of TMAP/CKAP2 in regulation of spindle function and cytokinesis.

Molecular and cellular biology

Hong KU,Park YS,Seong YS,Kang D,Bae CD,Park J

26183-HRP was used in western blot to study the role of TMAP/CKAP2 in mitotic spindle assembly and maintenance

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: HA; HA Epitope Tag; HA Tag; HA-tag; hemagglutinin

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HA Tag Monoclonal Antibody (2-2.2.14), HRP

HA Tag Antibody (26183-HRP) in WB

Product Details

26183-HRP

Western Blot (WB)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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