Invitrogen

HLA-DR Monoclonal Antibody (TU36), Qdot™ 655

HLA-DR Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 3

HLA-DR Antibody (Q22158) in Flow

Histogram of gated human lymphocytes labeled with HLA-DR Mouse Anti-Human mAb (clone Tu36), Qdot® 655 Conjugate. Sample was acquired and analyzed using 405 nm excitation and a 660/20 band pass emission filter using a BD LSR II flow cytometer (BD Biosciences, San Jose, CA).

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Product Details

Q22158

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

TU36

Immunogen

Human HLA-DR

Conjugate

Qdot™ 655 Qdot™ 655 Qdot™ 655

Excitation/Emission Max

300/654 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2556514

Target Information

HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Characterization of HIV-induced remodeling reveals differences in infection susceptibility of memory CD4⁺ T cell subsets in vivo.

Cell reports

Xie G,Luo X,Ma T,Frouard J,Neidleman J,Hoh R,Deeks SG,Greene WC,Roan NR

Q22158 was used in Flow Cytometry to provide an atlas of the phenotypes of in vivo and in vitro HIV-susceptible cells.

Tue Apr 27 00:00:00 EDT 2021

Distinctive features of SARS-CoV-2-specific T cells predict recovery from severe COVID-19.

Cell reports

Neidleman J,Luo X,George AF,McGregor M,Yang J,Yun C,Murray V,Gill G,Greene WC,Vasquez J,Lee SA,Ghosn E,Lynch KL,Roan NR

Q22158 was used in Flow Cytometry to define the features of T cells from individuals hospitalized in the ICU for COVID-19, including some that recovered and some that died from disease.

Tue Jul 20 00:00:00 EDT 2021

mRNA vaccine-induced T cells respond identically to SARS-CoV-2 variants of concern but differ in longevity and homing properties depending on prior infection status.

eLife

Neidleman J,Luo X,McGregor M,Xie G,Murray V,Greene WC,Lee SA,Roan NR

Q22158 was used in flow cytometry to provide reassurance that vaccine-elicited T cells respond robustly to emerging viral variants, confirm that convalescents may not need a second vaccine dose, and suggest that vaccinated convalescents may have more persistent nasopharynx-homing SARS-CoV-2-specific T cells compared to their infection-naive counterparts.

Tue Oct 12 00:00:00 EDT 2021

Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir.

eLife

Neidleman J,Luo X,Frouard J,Xie G,Hsiao F,Ma T,Morcilla V,Lee A,Telwatte S,Thomas R,Tamaki W,Wheeler B,Hoh R,Somsouk M,Vohra P,Milush J,James KS,Archin NM,Hunt PW,Deeks SG,Yukl SA,Palmer S,Greene WC,Roan NR

Q22158 was used in Flow cytometry/Cell sorting to describe a method that leverages high-dimensional phenotyping using CyTOF to trace latently infected cells reactivated ex vivo to their original pre-activation states.

Tue Sep 29 00:00:00 EDT 2020

SARS-CoV-2-Specific T Cells Exhibit Phenotypic Features of Helper Function, Lack of Terminal Differentiation, and High Proliferation Potential.

Cell reports. Medicine

Neidleman J,Luo X,Frouard J,Xie G,Gill G,Stein ES,McGregor M,Ma T,George AF,Kosters A,Greene WC,Vasquez J,Ghosn E,Lee S,Roan NR

Q22158 was used in Mass Spectrometry to show that long-lived and robust T cell immunity is generated following natural SARS-CoV-2 infection and support an important role of SARS-CoV-2-specific T cells in host control of COVID-19.

Tue Sep 22 00:00:00 EDT 2020

Multiparameter Single Cell Profiling of Airway Inflammatory Cells.

Cytometry. Part B, Clinical cytometry

Yao Y,Welp T,Liu Q,Niu N,Wang X,Britto CJ,Krishnaswamy S,Chupp GL,Montgomery RR

Q22158 was used in flow cytometry to perform a Cytometry by Time-Of-Flight analysis on primary airway samples obtained from well-characterized patients with asthma and cystic fibrosis

Sun Jan 01 00:00:00 EST 2017

Multiparameter Single Cell Profiling of Airway Inflammatory Cells.

Cytometry. Part B, Clinical cytometry

Yao Y,Welp T,Liu Q,Niu N,Wang X,Britto CJ,Krishnaswamy S,Chupp GL,Montgomery RR

Q22158 was used in flow cytometry to perform a Cytometry by Time-Of-Flight analysis on primary airway samples obtained from well-characterized patients with asthma and cystic fibrosis

Sun Jan 01 00:00:00 EST 2017

Increased Steady-State Memory B Cell Subsets Among High-Risk Participants in an HIV Vaccine Trial.

AIDS research and human retroviruses

Keefer MC,Zheng B,Rosenberg AF,Kobie JJ

Q22158 was used in flow cytometry to investigate how differences in B cell populations may contribute to HIV susceptibility

Mon Dec 18 00:00:00 EST 2017

Bioinformatics

Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; DASS-397D15.1; DR beta-5; DR-9; DR2-beta-2; DR7; DR9; DRB1 transplantation antigen; Dw2; DW2.2/DR2.2; FLJ51114; FLJ75017; FLJ76359; gamma chain of class II antigens; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen gamma chain; HLA class II histocompatibility antigen, DR alpha chain; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DR beta 4 chain; HLA class II histocompatibility antigen, DR beta 5 chain; HLA class II histocompatibility antigen, DR-1 beta chain; HLA class II histocompatibility antigen, DR-5 beta chain; HLA class II histocompatibility antigen, DRB1 beta chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA class II histocompatibility antigen, DRB1-9 beta chain; HLA-DR antigens-associated invariant chain; HLA-DR-gamma; HLA-DRB1; HLA-DRB5; human leucocyte antigen DRB1; human leucocyte antigen DRB3; human leucocyte antigen DRB4; human leucocyte antigen DRB5; Human leukocyte antigen DRB1; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; leukocyte antigen; leukocyte antigen class II; lymphocyte antigen DRB1; MHC cell surface glycoprotein; MHC class I antigen; MHC Class II antigen; MHC class II antigen DR beta 3 chain; MHC class II antigen DRA; MHC class II antigen DRB1*9; MHC class II antigen DRB3; MHC class II antigen DRB4; MHC class II antigen DRB5; MHC class II antigen HLA-DR-beta; MHC class II HLA beta chain; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR beta 3 chain; MHC class II HLA-DR-beta cell surface glycoprotein; MHC class II HLA-DRw10-beta; MHC class2 antigen; MHC HLA DR-beta chain; MHC HLA-DR gamma chain; MHC HLA-DR-beta cell surface glycoprotein; MHC HLA-DR-beta chain; p33; XXbac-BPG161M6.1

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Gene Aliases: CD74; DHLAG; DR-4; DR4; DRB1; DRB4; DRw10; HLA-DR1B; HLA-DR3B; HLA-DR4B; HLA-DRA; HLA-DRA1; HLA-DRB; HLA-DRB1; HLA-DRB3; HLA-DRB4; HLA-DRB5; HLADG; Ia-GAMMA; II; MLRW; SS1

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UniProt ID: (Human) P04233, (Human) P01903, (Human) Q5UBA2, (Human) P79483, (Human) P13762, (Human) Q30154

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Entrez Gene ID: (Human) 972, (Human) 3122, (Human) 3123, (Human) 3125, (Human) 3126, (Human) 3127

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Function(s)

beta-amyloid binding cytokine receptor activity protein binding cytokine binding MHC class II protein complex binding macrophage migration inhibitory factor binding MHC class II protein binding MHC class II protein binding, via antigen binding groove identical protein binding protein binding involved in protein folding nitric-oxide synthase binding MHC class II receptor activity peptide antigen binding major histocompatibility complex protein scaffold/adaptor protein

Process(es)

activation of MAPK activity prostaglandin biosynthetic process positive regulation of cytokine-mediated signaling pathway positive regulation of dendritic cell antigen processing and presentation negative regulation of peptide secretion positive regulation of type 2 immune response negative regulation of mature B cell apoptotic process protein complex assembly intracellular protein transport defense response signal transduction cell proliferation immunoglobulin mediated immune response antigen processing and presentation of endogenous antigen antigen processing and presentation of exogenous peptide antigen via MHC class II positive regulation of B cell proliferation macrophage migration inhibitory factor signaling pathway regulation of macrophage activation negative regulation of apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator T cell selection positive thymic T cell selection negative thymic T cell selection negative regulation of T cell differentiation positive regulation of T cell differentiation positive regulation of fibroblast proliferation positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration chaperone mediated protein folding requiring cofactor positive regulation of macrophage cytokine production positive regulation of ERK1 and ERK2 cascade positive regulation of neutrophil chemotaxis negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of chemokine (C-X-C motif) ligand 2 production peptide antigen assembly with MHC class II protein complex antigen processing and presentation of peptide or polysaccharide antigen via MHC class II polysaccharide assembly with MHC class II protein complex immune response viral process T cell costimulation T cell receptor signaling pathway cognition interferon-gamma-mediated signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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HLA-DR Monoclonal Antibody (TU36), Qdot™ 655

HLA-DR Antibody (Q22158) in Flow

Product Details

Q22158

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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