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Invitrogen
IFN gamma Monoclonal Antibody (MD-1), Functional Grade, eBioscience™
Product Details
16-7317-85
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MD-1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469249
Product Specific Information
Description: The MD-1 antibody reacts with human, rhesus, cynomolgus and baboon interferon-gamma (IFN gamma), the signature Th1 cytokine, which is produced mainly by activated T cells, NK cells and B cells. IFN gamma is a dimeric protein. IFN gamma has antiviral and antiparasitic activities and also inhibits the proliferation of normal and transformed cells.
Applications Reported: The MD-1 antibody has been reported for use in intracellular staining and flow cytometric analysis, cytokine neutralization, immunohistochemical staining, ELISA Capture, and ELISPOT Capture. Fluorochrome conjugated 4S.B3 antibody is recommended for use in intracellular staining and flow cytometry.
Applications Tested: The MD-1 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human interferon gamma (IFN gamma) in combination with the biotinylated 4S.B3 (13-7319) antibody for detection and recombinant human IFN gamma (39-8319) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/mL - 8 pg/mL should be included in each ELISA plate.
The FG Purified format of the MD-1 antibody has been tested as the capture antibody in an ELISPOT assay (in conjunction with the biotinylated 4S.B3 antibody) and for neutralization of IFN gamma biological activity. A suitable range of concentrations of this antibody for ELISPOT capture is 1-4 µg/mL.
For in vitro neutralization, the MD-1 antibody at 30 µg/mL has been found to neutralize by 50% the effect of 1 ng/mL human IFN gamma. A more potent neutralizing antibody is clone NIB42.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Immune interferon; Interferon gamma; Interferon y; Interferon γ; interferon, gamma; Interferonγ; M-IFN-g; R-IFN-g
Gene Aliases: IFG; IFI; IFNG
UniProt ID: (Human) P01579
Entrez Gene ID: (Human) 3458
protein import into nucleus, translocation
negative regulation of transcription from RNA polymerase II promoter
neutrophil apoptotic process
regulation of the force of heart contraction
adaptive immune response
CD8-positive, alpha-beta T cell differentiation involved in immune response
apoptotic process
movement of cell or subcellular component
humoral immune response
cell cycle arrest
cell surface receptor signaling pathway
positive regulation of cell proliferation
response to virus
positive regulation of autophagy
positive regulation of gene expression
negative regulation of gene expression
positive regulation of epithelial cell migration
antigen processing and presentation
neutrophil chemotaxis
negative regulation of epithelial cell differentiation
endoplasmic reticulum unfolded protein response
negative regulation of myelination
positive regulation of synaptic transmission, cholinergic
negative regulation of interleukin-17 production
positive regulation of interleukin-12 production
positive regulation of interleukin-23 production
positive regulation of tumor necrosis factor production
positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation involved in immune response
positive regulation of peptidyl-serine phosphorylation of STAT protein
positive regulation of smooth muscle cell apoptotic process
positive regulation of T cell proliferation
response to drug
positive regulation of tyrosine phosphorylation of Stat1 protein
defense response to bacterium
defense response to protozoan
negative regulation of growth of symbiont in host
positive regulation of chemokine biosynthetic process
positive regulation of interleukin-12 biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of nitric oxide biosynthetic process
positive regulation of neuron differentiation
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgG isotypes
negative regulation of smooth muscle cell proliferation
positive regulation of interleukin-1 beta secretion
regulation of insulin secretion
T cell receptor signaling pathway
sensory perception of mechanical stimulus
positive regulation of membrane protein ectodomain proteolysis
defense response to virus
positive regulation of killing of cells of other organism
interferon-gamma-mediated signaling pathway
regulation of interferon-gamma-mediated signaling pathway
positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
positive regulation of fructose 1,6-bisphosphate metabolic process
positive regulation of vitamin D biosynthetic process
positive regulation of calcidiol 1-monooxygenase activity
cellular response to lipopolysaccharide
cellular response to interleukin-18
positive regulation of establishment of protein localization to plasma membrane
extrinsic apoptotic signaling pathway
regulation of neuronal action potential
positive regulation of exosomal secretion
positive regulation of tumor necrosis factor (ligand) superfamily member 11 production
regulation of hepatocyte proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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