Invitrogen

IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

22 Published Figures

29 References

View all (7) IL-1 beta (Pro-form) antibodies

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Datasheet

Protocols

Questions & Answers

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IL-1 beta (Pro-form) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 24

IL-1 beta (Pro-form) Antibody (12-7114-82) in Flow

In vitro-cultured mouse monocytes unstimulated (left) or stimulated with LPS (right), in the presence of Protein Transport Inhibitor Cocktail (Product # 00-4980-03), stained with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.03 µg of Anti-Mouse IL-1 beta Pro-form PE. Total viable cells were used for analysis.

IL-1 beta (Pro-form) Antibody in Immunocytochemistry (ICC/IF)

Product Image Gallery

Product Details

12-7114-82

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.06 µg/test

View 28 publications 28 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

NJTEN3

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10732630

Product Specific Information

Description: This NJTEN3 monoclonal antibody reacts with the pro-form of mouse IL-1 beta, which is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. IL-1 beta signals via the IL-1RI, which is shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.The NJTEN3 antibody recognizes only the pro-form of mouse IL-1 beta and does not see the active (cleaved) form.

Applications Reported: This NJTEN3 antibody has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: This NJTEN3 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thioglycolate-elicited peritoneal macrophages using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. It is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is instead secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions.IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. These cytokines play important roles in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses. Their activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions, giving IL-RA or IL-1 beta neutralizing antibodies potential therapeutical value.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Zika Virus Infection Induces Interleukin-1β-Mediated Inflammatory Responses by Macrophages in the Brain of an Adult Mouse Model.

Journal of virology

Jeong GU,Lee S,Kim DY,Lyu J,Yoon GY,Kim KD,Ku KB,Ko J,Kwon YC

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Thu Jun 29 00:00:00 EDT 2023

Inhibition of autophagy in microglia and macrophages exacerbates innate immune responses and worsens brain injury outcomes.

Autophagy

Hegdekar N,Sarkar C,Bustos S,Ritzel RM,Hanscom M,Ravishankar P,Philkana D,Wu J,Loane DJ,Lipinski MM

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2023

Microbially-Induced Exosomes from Dendritic Cells Promote Paracrine Immune Senescence: Novel Mechanism of Bone Degenerative Disease in Mice.

Aging and disease

Elsayed R,Elashiry M,Liu Y,Morandini AC,El-Awady A,Elashiry MM,Hamrick M,Cutler CW

12-7114-82 was used in Flow cytometry/Cell sorting to conclude that immune senescence, compounded by advanced age, and accelerated by oral dysbiosis and its induced SASP exosomes, plays a pivotal role in the pathophysiology of experimental periodontitis.

Wed Feb 01 00:00:00 EST 2023

Brain innate immune response via miRNA-TLR7 sensing in polymicrobial sepsis.

Brain, behavior, and immunity

Zou L,He J,Gu L,Shahror RA,Li Y,Cao T,Wang S,Zhu J,Huang H,Chen F,Fan X,Wu J,Chao W

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Tue Feb 01 00:00:00 EST 2022

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

12-7114-82 was used in Flow cytometry/Cell sorting to investigate the role of TNF-like ligand 1 aberrance (TL1A) in nonalcoholic steatohepatitis (NASH).

Fri Mar 04 00:00:00 EST 2022

TNF-Like Ligand 1 Aberrance Aggravates Nonalcoholic Steatohepatitis via M1 Macrophage Polarization.

Oxidative medicine and cellular longevity

Luo Y,Guo J,Jia W,Wu M,Yin F,Niu G,Shih DQ,Targan SR,Zhang X

12-7114-82 was used in Flow cytometry/Cell sorting to investigate the role of TNF-like ligand 1 aberrance (TL1A) in nonalcoholic steatohepatitis (NASH).

Fri Mar 04 00:00:00 EST 2022

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway.

Journal of neuroinflammation

Duan RN,Yang CL,Du T,Liu A,Wang AR,Sun WJ,Li X,Li JX,Yan CZ,Liu QJ

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway.

Journal of neuroinflammation

Duan RN,Yang CL,Du T,Liu A,Wang AR,Sun WJ,Li X,Li JX,Yan CZ,Liu QJ

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

Smek1 deficiency exacerbates experimental autoimmune encephalomyelitis by activating proinflammatory microglia and suppressing the IDO1-AhR pathway.

Journal of neuroinflammation

Duan RN,Yang CL,Du T,Liu A,Wang AR,Sun WJ,Li X,Li JX,Yan CZ,Liu QJ

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Mon Jun 28 00:00:00 EDT 2021

NLRP3 Inflammasome Activation of Mast Cells by Estrogen via the Nuclear-Initiated Signaling Pathway Contributes to the Development of Endometriosis.

Frontiers in immunology

Guo X,Xu X,Li T,Yu Q,Wang J,Chen Y,Ding S,Zhu L,Zou G,Zhang X

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Tue Jan 18 00:00:00 EST 2022

MicroRNA-326 attenuates immune escape and prevents metastasis in lung adenocarcinoma by targeting PD-L1 and B7-H3.

Cell death discovery

Shao L,He Q,Wang J,He F,Lin S,Wu L,Gao Y,Ma W,Dong J,Yang X,Li F

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Tue Jun 15 00:00:00 EDT 2021

Fructose reprogrammes glutamine-dependent oxidative metabolism to support LPS-induced inflammation.

Nature communications

Jones N,Blagih J,Zani F,Rees A,Hill DG,Jenkins BJ,Bull CJ,Moreira D,Bantan AIM,Cronin JG,Avancini D,Jones GW,Finlay DK,Vousden KH,Vincent EE,Thornton CA

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Mon Feb 22 00:00:00 EST 2021

Interleukin-1β Suppresses Gastrin via Primary Cilia and Induces Antral Hyperplasia.

Cellular and molecular gastroenterology and hepatology

Ding L,Sontz EA,Saqui-Salces M,Merchant JL

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

The Intermucosal Connection between the Mouth and Gut in Commensal Pathobiont-Driven Colitis.

Cell

Kitamoto S,Nagao-Kitamoto H,Jiao Y,Gillilland MG,Hayashi A,Imai J,Sugihara K,Miyoshi M,Brazil JC,Kuffa P,Hill BD,Rizvi SM,Wen F,Bishu S,Inohara N,Eaton KA,Nusrat A,Lei YL,Giannobile WV,Kamada N

12-7114-82 was used in Flow Cytometry to conclude that oral inflammation, such as periodontitis, exacerbates gut inflammation by supplying the gut with both colitogenic pathobionts and pathogenic T cells.

Thu Jul 23 00:00:00 EDT 2020

Endogenous oxidized phospholipids reprogram cellular metabolism and boost hyperinflammation.

Nature immunology

Di Gioia M,Spreafico R,Springstead JR,Mendelson MM,Joehanes R,Levy D,Zanoni I

12-7114 was used in Flow Cytometry to report that the self-encoded oxidized phospholipid oxPAPC alters the metabolism of macrophages exposed to lipopolysaccharide.

Wed Jan 01 00:00:00 EST 2020

Hippocampal interleukin-33 mediates neuroinflammation-induced cognitive impairments.

Journal of neuroinflammation

Reverchon F,de Concini V,Larrigaldie V,Benmerzoug S,Briault S,Togbé D,Ryffel B,Quesniaux VFJ,Menuet A

12-7114 was used in Flow cytometry/Cell sorting to investigate the role of Interleukin (IL)-33 in immunoregulatory mechanisms in the brain.

Fri Sep 11 00:00:00 EDT 2020

Hippocampal interleukin-33 mediates neuroinflammation-induced cognitive impairments.

Journal of neuroinflammation

Reverchon F,de Concini V,Larrigaldie V,Benmerzoug S,Briault S,Togbé D,Ryffel B,Quesniaux VFJ,Menuet A

12-7114 was used in Flow cytometry/Cell sorting to investigate the role of Interleukin (IL)-33 in immunoregulatory mechanisms in the brain.

Fri Sep 11 00:00:00 EDT 2020

Triacylglycerol synthesis enhances macrophage inflammatory function.

Nature communications

Castoldi A,Monteiro LB,van Teijlingen Bakker N,Sanin DE,Rana N,Corrado M,Cameron AM,Hässler F,Matsushita M,Caputa G,Klein Geltink RI,Büscher J,Edwards-Hicks J,Pearce EL,Pearce EJ

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Mannose receptor high, M2 dermal macrophages mediate nonhealing Leishmania major infection in a Th1 immune environment.

The Journal of experimental medicine

Lee SH,Charmoy M,Romano A,Paun A,Chaves MM,Cope FO,Ralph DA,Sacks DL

12-7114 was used in Flow cytometry/Cell sorting to study the origin and functional specialisation of dermal macrophages in cutaneous infections.

Tue Jan 02 00:00:00 EST 2018

1,25-Dihydroxyvitamin D suppresses M1 macrophages and promotes M2 differentiation at bone injury sites.

JCI insight

Wasnik S,Rundle CH,Baylink DJ,Yazdi MS,Carreon EE,Xu Y,Qin X,Lau KW,Tang X

12-7114 was used in Flow cytometry/Cell sorting to define the role of M1 macrophages at bone injury sites via the function of 1,25-Dihydroxyvitamin D in suppressing M1 but promoting M2 differentiation.

Thu Sep 06 00:00:00 EDT 2018

Dicer Deficiency Differentially Impacts Microglia of the Developing and Adult Brain.

Immunity

Varol D,Mildner A,Blank T,Shemer A,Barashi N,Yona S,David E,Boura-Halfon S,Segal-Hayoun Y,Chappell-Maor L,Keren-Shaul H,Leshkowitz D,Hornstein E,Fuhrmann M,Amit I,Maggio N,Prinz M,Jung S

12-7114 was used in Flow cytometry/Cell sorting to examine the impact of post-transcriptional control mediated by microRNAs on microglial performance during development and adulthood.

Tue Jun 20 00:00:00 EDT 2017

Cholesterol Accumulation in Dendritic Cells Links the Inflammasome to Acquired Immunity.

Cell metabolism

Westerterp M,Gautier EL,Ganda A,Molusky MM,Wang W,Fotakis P,Wang N,Randolph GJ,D'Agati VD,Yvan-Charvet L,Tall AR

12-7114 was used in Flow cytometry/Cell sorting to identify an essential role of DC cholesterol efflux pathways in maintaining immune tolerance.

Tue Jun 06 00:00:00 EDT 2017

Notch Regulates Macrophage-Mediated Inflammation in Diabetic Wound Healing.

Frontiers in immunology

Kimball AS,Joshi AD,Boniakowski AE,Schaller M,Chung J,Allen R,Bermick J,Carson WF,Henke PK,Maillard I,Kunkel SL,Gallagher KA

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

The amino acid sensor GCN2 controls gut inflammation by inhibiting inflammasome activation.

Nature

Ravindran R,Loebbermann J,Nakaya HI,Khan N,Ma H,Gama L,Machiah DK,Lawson B,Hakimpour P,Wang YC,Li S,Sharma P,Kaufman RJ,Martinez J,Pulendran B

12-7114 was used in Flow cytometry/Cell sorting to reveal a mechanism that couples amino acid sensing with control of intestinal inflammation via GCN2.

Thu Mar 24 00:00:00 EDT 2016

Neutrophils Oppose Uterine Epithelial Carcinogenesis via Debridement of Hypoxic Tumor Cells.

Cancer cell

Blaisdell A,Crequer A,Columbus D,Daikoku T,Mittal K,Dey SK,Erlebacher A

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Mon Dec 14 00:00:00 EST 2015

Blood-brain barrier dysfunction developed during normal aging is associated with inflammation and loss of tight junctions but not with leukocyte recruitment.

Immunity & ageing : I & A

Elahy M,Jackaman C,Mamo JC,Lam V,Dhaliwal SS,Giles C,Nelson D,Takechi R

Wed Mar 18 00:00:00 EDT 2015

Differential ASC requirements reveal a key role for neutrophils and a noncanonical IL-1β response to Pseudomonas aeruginosa.

American journal of physiology. Lung cellular and molecular physiology

Patankar YR,Mabaera R,Berwin B

12-7114 was used in Flow cytometry/Cell sorting to demonstrate the physiological contribution of neutrophils as a source of IL-1β in response to P. aeruginosa infection.

Thu Oct 15 00:00:00 EDT 2015

Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.

Nature medicine

Kim HY,Lee HJ,Chang YJ,Pichavant M,Shore SA,Fitzgerald KA,Iwakura Y,Israel E,Bolger K,Faul J,DeKruyff RH,Umetsu DT

12-7114 was used in Flow cytometry/Cell sorting to show that IL-17 producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.

Wed Jan 01 00:00:00 EST 2014

The frustrated host response to Legionella pneumophila is bypassed by MyD88-dependent translation of pro-inflammatory cytokines.

PLoS pathogens

Asrat S,Dugan AS,Isberg RR

12-7114 was used in Flow cytometry/Cell sorting to show how the protein translation inhibition induced by L. pneumophila is bypassed by MyD88, allowing pro-inflammatory cytokine translation.

Tue Jul 01 00:00:00 EDT 2014

Acidosis potentiates the host proinflammatory interleukin-1β response to Pseudomonas aeruginosa infection.

Infection and immunity

Torres IM,Patankar YR,Shabaneh TB,Dolben E,Hogan DA,Leib DA,Berwin BL

12-7114 was used in Flow cytometry/Cell sorting to show that extracellular pH governs the host inflammatory response against P. aeruginosa via synergistic mechanisms that modulate cytokine release.

Sat Nov 01 00:00:00 EDT 2014

Cell-selective inhibition of NF-κB signaling improves therapeutic index in a melanoma chemotherapy model.

Cancer discovery

Enzler T,Sano Y,Choo MK,Cottam HB,Karin M,Tsao H,Park JM

Published figure using IL-1 beta (Pro-form) monoclonal antibody (Product # 12-7114-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: il 1b; IL 1β; IL-1 beta; IL1β; ILN; Interleukin; Interleukin-1 beta; Interleukin-1b; Interleukin1 beta

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Gene Aliases: Il-1b; IL-1beta; Il1b

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UniProt ID: (Mouse) P10749

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Entrez Gene ID: (Mouse) 16176

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Function(s)

receptor binding cytokine activity interleukin-1 receptor binding protein domain specific binding interleukin superfamily

Process(es)

Performance Guarantee

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IL-1 beta (Pro-form) Monoclonal Antibody (NJTEN3), PE, eBioscience™

IL-1 beta (Pro-form) Antibody (12-7114-82) in Flow

Product Details

12-7114-82

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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