Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™

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IL-10 Antibody (12-7108-82) in Flow

Normal human peripheral blood cells were stimulated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours. The cells were surface stained with Anti-Human CD4 FITC (Product # 11-0049-42) and intracellularly stained with Anti-Human IL-10 PE.

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Product Details

12-7108-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 22 publications 22 publications

ELISA (ELISA)

View 1 publication 1 publication

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Fungi, Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466179

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported:JES3-9D7 has been reported for use in intracellular staining and flow cytometric analysis.

Applications Tested: Has been tested by intracellular staining and flow cytometric analysis of human activated cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Interleukin 6 exacerbates the progression of warm autoimmune hemolytic anemia by influencing the activity and function of B cells.

Scientific reports

Zhao M,Chen L,Yang J,Zhang Z,Wang H,Shao Z,Liu X,Xing L

12-7108-82 was used in Flow cytometry/Cell sorting to explore the effect of IL-6 on the activity and secretory function of B cells and analyze its effect on clinical indicators and efficacy in wAIHA patients.

Mon Aug 14 00:00:00 EDT 2023

Circulating Regulatory B-Lymphocytes in Patients with Acute Myocardial Infarction: A Pilot Study.

Journal of cardiovascular development and disease

Volodarsky I,Shimoni S,Haberman D,Mirkin V,Fabrikant Y,Yoskovich Mashriki T,Zalik A,George J

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

Fungal sensing by dectin-1 directs the non-pathogenic polarization of T_H17 cells through balanced type I IFN responses in human DCs.

Nature immunology

Gringhuis SI,Kaptein TM,Remmerswaal EBM,Drewniak A,Wevers BA,Theelen B,D'Haens GRAM,Boekhout T,Geijtenbeek TBH

12-7108-82 was used in Flow Cytometry to show that dectin-1-mediated modulation of type I IFN responses allowed TGF-β activation and non-pathogenic TH17 cell development during fungal infections in humans.

Thu Dec 01 00:00:00 EST 2022

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens.

Frontiers in cellular and infection microbiology

Fan X,Zhang Y,Ouyang R,Luo B,Li L,He W,Liu M,Jiang N,Yang F,Wang L,Zhou B

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Altered T-Cell Subsets are Associated with Dysregulated Cytokine Secretion of CD4⁺ T Cells During HIV Infection.

Journal of inflammation research

Wang D,Jiang Y,Song Y,Zeng Y,Li C,Wang X,Liu Y,Xiao J,Kong Y,Zhao H

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

A human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism.

Nature medicine

Trotta E,Bessette PH,Silveria SL,Ely LK,Jude KM,Le DT,Holst CR,Coyle A,Potempa M,Lanier LL,Garcia KC,Crellin NK,Rondon IJ,Bluestone JA

12-7108-82 was used in Flow Cytometry to suggest that IL-2-F5111.2 may provide an immunotherapy to treat autoimmune diseases and graft-versus-host disease.

Sun Jul 01 00:00:00 EDT 2018

Regulatory T cells as a biomarker for response to adalimumab in rheumatoid arthritis.

The Journal of allergy and clinical immunology

Nguyen DX,Cotton A,Attipoe L,Ciurtin C,Doré CJ,Ehrenstein MR

12-7108 was used in Flow cytometry/Cell sorting to determine whether adalimumab's ability to boost Treg cells in vitro using PBMCs before treatment would predict clinical response.

Sat Sep 01 00:00:00 EDT 2018

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Microbiota-derived short-chain fatty acids promote Th1 cell IL-10 production to maintain intestinal homeostasis.

Nature communications

Sun M,Wu W,Chen L,Yang W,Huang X,Ma C,Chen F,Xiao Y,Zhao Y,Ma C,Yao S,Carpio VH,Dann SM,Zhao Q,Liu Z,Cong Y

12-7108-82 was used in Flow Cytometry to elucidate the various roles of gut microbiota-derived short-chain fatty acids in the maintenance of intestinal homeostasis.

Mon Sep 03 00:00:00 EDT 2018

Schistosoma mansoni rSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis.

Frontiers in immunology

Lopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS

12-7108 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis.

Tue Oct 15 00:00:00 EDT 2019

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

12-7108 was used in Microarray to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 EDT 2017

Decidual T Cells Exhibit a Highly Differentiated Phenotype and Demonstrate Potential Fetal Specificity and a Strong Transcriptional Response to IFN.

Journal of immunology (Baltimore, Md. : 1950)

Powell RM,Lissauer D,Tamblyn J,Beggs A,Cox P,Moss P,Kilby MD

12-7108 was used in Flow cytometry/Cell sorting to investigate the specificity and regulation of CD4+ and CD8+ T cells obtained from human third trimester decidua and demonstrated that decidual CD4+ and CD8+ T cells exhibit a highly differentiated effector memory phenotype in comparison with peripheral blood.

Wed Nov 15 00:00:00 EST 2017

Azacytidine prevents experimental xenogeneic graft-versus-host disease without abrogating graft-versus-leukemia effects.

Oncoimmunology

Ehx G,Fransolet G,de Leval L,D'Hondt S,Lucas S,Hannon M,Delens L,Dubois S,Drion P,Beguin Y,Humblet-Baron S,Baron F

12-7108 was used in Flow cytometry/Cell sorting to investigate the impact of 5-azacytidine on xenogeneic graft-vs.-host disease and graft-vs.-leukaemia effects in a humanised murine model of transplantation.

Tue Jan 12 00:00:00 EST 2021

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Renal Transplant Recipients Treated with Calcineurin-Inhibitors Lack Circulating Immature Transitional CD19+CD24hiCD38hi Regulatory B-Lymphocytes.

PloS one

Tebbe B,Wilde B,Ye Z,Wang J,Wang X,Jian F,Dolff S,Schedlowski M,Hoyer PF,Kribben A,Witzke O,Hoerning A

12-7108 was used in Flow cytometry/Cell sorting to analyse the effect of calcineurin inhibition on peripherally circulating regulatory B cells in renal transplant recipients receiving cyclosporine A or tacrolimus.

Mon Aug 29 00:00:00 EDT 2016

CD26-mediated induction of EGR2 and IL-10 as potential regulatory mechanism for CD26 costimulatory pathway.

Journal of immunology (Baltimore, Md. : 1950)

Hatano R,Ohnuma K,Otsuka H,Komiya E,Taki I,Iwata S,Dang NH,Okumura K,Morimoto C

12-7108 was used in Flow cytometry/Cell sorting to investigate negative feedback mechanisms to regulate CD26-mediated T cell activation.

Sun Feb 01 00:00:00 EST 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using IL-10 monoclonal antibody (Product # 12-7108-82) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Hyperreactive onchocerciasis is characterized by a combination of Th17-Th2 immune responses and reduced regulatory T cells.

PLoS neglected tropical diseases

Katawa G,Layland LE,Debrah AY,von Horn C,Batsa L,Kwarteng A,Arriens S,W Taylor D,Specht S,Hoerauf A,Adjobimey T

12-7108 was used in Flow cytometry/Cell sorting to provide initial evidence that elevated frequencies of Th17 and Th2 cells form part of the immune network instigating the development of severe onchocerciasis.

Thu Jan 01 00:00:00 EST 2015

The IL-10 polarized cytokine pattern in innate and adaptive immunity cells contribute to the development of FVIII inhibitors.

BMC hematology

Silveira AC,Santana MA,Ribeiro IG,Chaves DG,Martins-Filho OA

12-7108 was used in Flow cytometry/Cell sorting to understand immune response to FVIII in patients with HA who were either positive [HAα-FVIII(+)] or negative [HAα-FVIII(-)] for inhibitors.

Thu Feb 05 00:00:00 EST 2015

The anti-idiotypic antibody 1F7 stimulates monocyte interleukin-10 production and induces endotoxin tolerance.

Journal of inflammation (London, England)

Davtyan TK,Poghosyan DA,Sukiasyan AG,Grant MD

12-7108 was used in Flow cytometry/Cell sorting to investigate the direct effects of the 1F7 mAb on peripheral blood mononuclear cells.

Fri Apr 05 00:00:00 EDT 2013

Differential IL-7 responses in developing human thymocytes.

Human immunology

Marino JH,Tan C,Taylor AA,Bentley C,Van De Wiele CJ,Ranne R,Paliotta M,Broughan TA,Teague TK

12-7108 was used in Flow cytometry/Cell sorting to assess IL-7 receptor expression and IL-7 signalling in human thymocytes.

Thu Apr 01 00:00:00 EDT 2010

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

Tue May 01 00:00:00 EDT 2007

Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.

Journal of immunology (Baltimore, Md. : 1950)

Sendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™

IL-10 Antibody (12-7108-82) in Flow

Product Details

12-7108-82

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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