Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), Functional Grade, eBioscience™

IL-10 Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 14

IL-10 Antibody (16-7108-81) in IHC

Saudi journal of biological sciences 2022 - Histological changes of cervical rats on IL-10 expression by Z. acanthopodium methanol extracts. a. Control, b. Cancer rats without treatments, c. Cancer rats + 100 mg/Kg BW of ZAM, d. Cancer rats + 200 mg/Kg BW of ZAM, e.Cancer rats + 400 mg/Kg BW of ZAM. red arrows: positive IL-10 (400x).

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Product Details

16-7108-81

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

View 12 publications 12 publications

ELISA (ELISA)

Assay-Dependent

View 6 publications 6 publications

Neutralization (Neu)

Assay-Dependent

View 2 publications 2 publications

Functional Assay (Functional)

Assay-Dependent

View 2 publications 2 publications

Inhibition Assays (IA)

View 2 publications 2 publications

In vitro Assay (IV)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469228

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: The JES3-9D7 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The JES3-9D7 antibody has been tested for neutralizing IL-10 binding and biological activity.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Histological changes of cervical tumours following Zanthoxylum acanthopodium DC treatment, and its impact on cytokine expression.

Saudi journal of biological sciences

Simanullang RH,Situmorang PC,Herlina M,Silalahi B,Manurung SS

16-7108-81 was used in ELISA to conclude that ZAM may be a potential target for molecular cytokine therapy for cervical cancer.

Fri Apr 01 00:00:00 EDT 2022

Histological changes of cervical tumours following Zanthoxylum acanthopodium DC treatment, and its impact on cytokine expression.

Saudi journal of biological sciences

Simanullang RH,Situmorang PC,Herlina M,Silalahi B,Manurung SS

16-7108-81 was used in ELISA to conclude that ZAM may be a potential target for molecular cytokine therapy for cervical cancer.

Fri Apr 01 00:00:00 EDT 2022

Circulating Regulatory B-Lymphocytes in Patients with Acute Myocardial Infarction: A Pilot Study.

Journal of cardiovascular development and disease

Volodarsky I,Shimoni S,Haberman D,Mirkin V,Fabrikant Y,Yoskovich Mashriki T,Zalik A,George J

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells.

Cancer immunology, immunotherapy : CII

Sadeghlar F,Vogt A,Mohr RU,Mahn R,van Beekum K,Kornek M,Weismüller TJ,Branchi V,Matthaei H,Toma M,Schmidt-Wolf IGH,Kalff JC,Strassburg CP,González-Carmona MA

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in ELISA

Sat May 01 00:00:00 EDT 2021

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens.

Frontiers in cellular and infection microbiology

Fan X,Zhang Y,Ouyang R,Luo B,Li L,He W,Liu M,Jiang N,Yang F,Wang L,Zhou B

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Altered T-Cell Subsets are Associated with Dysregulated Cytokine Secretion of CD4⁺ T Cells During HIV Infection.

Journal of inflammation research

Wang D,Jiang Y,Song Y,Zeng Y,Li C,Wang X,Liu Y,Xiao J,Kong Y,Zhao H

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

CD19-targeted CAR regulatory T cells suppress B cell pathology without GvHD.

JCI insight

Imura Y,Ando M,Kondo T,Ito M,Yoshimura A

16-7108-81 was used in In vitro Assay to suggest that CD19-CAR regulatory T cells can treat autoantibody-mediated autoimmune diseases by suppressing B cell pathology.

Thu Jul 23 00:00:00 EDT 2020

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Description of organ-specific phenotype, and functional characteristics of tissue resident lymphocytes from liver transplantation donor and research on immune tolerance mechanism of liver.

Oncotarget

Shi Y,Zhang P,Wang G,Liu X,Sun X,Zhang X,Li H,Qi J,Ding L,Li T,Zhang R,Chen Y,Zhou J,Lv G,Tu Z

16-7108 was used in Flow cytometry to study the phenotype and function of tissue resident lymphocytes in peripheral blood, lymph node, spleen and liver.

Tue Mar 20 00:00:00 EDT 2018

PKCζ in prostate cancer cells represses the recruitment and M2 polarization of macrophages in the prostate cancer microenvironment.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Fan HH,Li L,Zhang YM,Yang J,Li MC,Zeng FY,Deng F

16-7108 was used in an ELISA assay to suggest that protein kinase C zeta plays an important role in reducing infiltration of tumor-associated macrophages and activation of a pro-tumor M2 phenotype, which may constitute an important mechanism by which protein kinase C zeta represses cancer progression.

Thu Jun 01 00:00:00 EDT 2017

Helicobacter pylori-Mediated Protection from Allergy Is Associated with IL-10-Secreting Peripheral Blood Regulatory T Cells.

Frontiers in immunology

Hussain K,Letley DP,Greenaway AB,Kenefeck R,Winter JA,Tomlinson W,Rhead J,Staples E,Kaneko K,Atherton JC,Robinson K

16-7108 was used in Blocking experiments to suggest a role for systemic IL-10+ Treg response in Helicobacter pylori-mediated protection against allergy in humans.

Fri Mar 25 00:00:00 EDT 2016

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2016

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2016

The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.

Oncology letters

Wang Y,Yi J,Chen X,Zhang Y,Xu M,Yang Z

16-7108 was used in Blocking experiments to investigate the role of exosomes in tumourigenesis.

Mon Feb 01 00:00:00 EST 2016

The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.

Oncology letters

Wang Y,Yi J,Chen X,Zhang Y,Xu M,Yang Z

16-7108 was used in Blocking experiments to investigate the role of exosomes in tumourigenesis.

Mon Feb 01 00:00:00 EST 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using IL-10 monoclonal antibody (Product # 16-7108-81) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Activated platelets enhance IL-10 secretion and reduce TNF-α secretion by monocytes.

Journal of immunology (Baltimore, Md. : 1950)

Gudbrandsdottir S,Hasselbalch HC,Nielsen CH

16-7108 was used in Blocking experiments to suggest that activated platelets have anti-inflammatory properties related to the interaction between CD40L and CD40.

Tue Oct 15 00:00:00 EDT 2013

The role of JAK-3 in regulating TLR-mediated inflammatory cytokine production in innate immune cells.

Journal of immunology (Baltimore, Md. : 1950)

Wang H,Brown J,Gao S,Liang S,Jotwani R,Zhou H,Suttles J,Scott DA,Lamont RJ

16-7108 was used in Neutralization experiments to demonstrate the signaling pathway by which JAK3 differentially regulates TLR-mediated inflammatory cytokine production in innate immune cells.

Thu Aug 01 00:00:00 EDT 2013

Bacterial reprogramming of PBMCs impairs monocyte phagocytosis and modulates adaptive T cell responses.

Journal of leukocyte biology

André MC,Gille C,Glemser P,Woiterski J,Hsu HY,Spring B,Keppeler H,Kramer BW,Handgretinger R,Poets CF,Lauber K,Orlikowsky TW

16-7108 was used in Functional assays to characterise bacterially reprogrammed peripheral blood mononuclear cell-derived monocytes, with specific focus on their phagocytic activity.

Fri Jun 01 00:00:00 EDT 2012

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells.

Nature medicine

Ma C,Fan R,Ahmad H,Shi Q,Comin-Anduix B,Chodon T,Koya RC,Liu CC,Kwong GA,Radu CG,Ribas A,Heath JR

16-7108 was used in Flow cytometry/Cell sorting to develop a microchip for quantitative measurement of secreted proteins from single cells.

Wed Jun 01 00:00:00 EDT 2011

Thymic stromal lymphopoietin fosters human breast tumor growth by promoting type 2 inflammation.

The Journal of experimental medicine

Pedroza-Gonzalez A,Xu K,Wu TC,Aspord C,Tindle S,Marches F,Gallegos M,Burton EC,Savino D,Hori T,Tanaka Y,Zurawski S,Zurawski G,Bover L,Liu YJ,Banchereau J,Palucka AK

16-7108 was used in Flow cytometry/Cell sorting to study how breast cancer cell-derived TSLP contributes to the inflammatory Th2 microenvironment conducive to breast tumor development by inducing OX40L expression on DCs.

Mon Mar 14 00:00:00 EDT 2011

Human TCR transgenic Bet v 1-specific Th1 cells suppress the effector function of Bet v 1-specific Th2 cells.

Journal of immunology (Baltimore, Md. : 1950)

Neunkirchner A,Leb-Reichl VM,Schmetterer KG,Mutschlechner S,Kueng HJ,Haiderer D,Schuch K,Wallner M,Jahn-Schmid B,Bohle B,Pickl WF

16-7108 was used in Flow cytometry/Cell sorting to study the mechanisms underlying the cross-reactivity between the respiratory allergen, Bet v 1, and its homologous food allergens.

Sat Oct 15 00:00:00 EDT 2011

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

Tue May 01 00:00:00 EDT 2007

Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.

Journal of immunology (Baltimore, Md. : 1950)

Sendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (JES3-9D7), Functional Grade, eBioscience™

IL-10 Antibody (16-7108-81) in IHC

Product Details

16-7108-81

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

In vitro Assay (IV)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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