Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), eFluor™ 660, eBioscience™

FIGURE: 1 / 15

IL-10 Antibody (50-7108-42) in Flow

CD4+ human peripheral blood cells were polarized under Th2 conditions then were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03). Cells were intracellularly stained with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-10 eFluor® 660 using the Fixation & Permeabilization Buffers (Product # 88-8824-00). Total viable cells were used for analysis.

IL-10 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

50-7108-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 8 publications 8 publications

ELISA (ELISA)

View 4 publications 4 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

eFluor™ 660 eFluor™ 660 eFluor™ 660

Excitation/Emission Max

651/668 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11149363

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: This JES3-9D7 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining anf flow cytometryci analysis of Th2 polarized and stimulated human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Histological changes of cervical tumours following Zanthoxylum acanthopodium DC treatment, and its impact on cytokine expression.

Saudi journal of biological sciences

Simanullang RH,Situmorang PC,Herlina M,Silalahi B,Manurung SS

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2022

Circulating Regulatory B-Lymphocytes in Patients with Acute Myocardial Infarction: A Pilot Study.

Journal of cardiovascular development and disease

Volodarsky I,Shimoni S,Haberman D,Mirkin V,Fabrikant Y,Yoskovich Mashriki T,Zalik A,George J

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells.

Cancer immunology, immunotherapy : CII

Sadeghlar F,Vogt A,Mohr RU,Mahn R,van Beekum K,Kornek M,Weismüller TJ,Branchi V,Matthaei H,Toma M,Schmidt-Wolf IGH,Kalff JC,Strassburg CP,González-Carmona MA

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in ELISA

Sat May 01 00:00:00 EDT 2021

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens.

Frontiers in cellular and infection microbiology

Fan X,Zhang Y,Ouyang R,Luo B,Li L,He W,Liu M,Jiang N,Yang F,Wang L,Zhou B

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Altered T-Cell Subsets are Associated with Dysregulated Cytokine Secretion of CD4⁺ T Cells During HIV Infection.

Journal of inflammation research

Wang D,Jiang Y,Song Y,Zeng Y,Li C,Wang X,Liu Y,Xiao J,Kong Y,Zhao H

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

50-7108 was used in Flow cytometry/Cell sorting to provide a comprehensive understanding of the mechanisms of CAIX in the GBM microenvironment.

Fri Aug 14 00:00:00 EDT 2020

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

50-7108 was used in Flow cytometry/Cell sorting to provide a comprehensive understanding of the mechanisms of CAIX in the GBM microenvironment.

Fri Aug 14 00:00:00 EDT 2020

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2016

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Immunofluorescence

Thu Sep 01 00:00:00 EDT 2016

The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.

Oncology letters

Wang Y,Yi J,Chen X,Zhang Y,Xu M,Yang Z

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in ELISA

Mon Feb 01 00:00:00 EST 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using IL-10 monoclonal antibody (Product # 50-7108-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

Tue May 01 00:00:00 EDT 2007

Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.

Journal of immunology (Baltimore, Md. : 1950)

Sendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

View more View less

Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

View more View less

UniProt ID: (Human) P22301

View more View less

Entrez Gene ID: (Human) 3586

View more View less

Function(s)

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-10 Monoclonal Antibody (JES3-9D7), eFluor™ 660, eBioscience™

IL-10 Antibody (50-7108-42) in Flow

Product Details

50-7108-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!