Invitrogen

IL-10 Monoclonal Antibody (JES3-9D7), Alexa Fluor™ 488, eBioscience™

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IL-10 Antibody (53-7108-42) in Flow

Intracellular staining of Th2-polarized cells restimulated with PMA/Ionomycin (right) or unstimulated (left) in the presence of monensin for 5 hrs. Cells were harvested, stained with Anti-Human CD3 PE (Product # 12-0037-42) and Anti-Human IL-10 Alexa Fluor® 488.

IL-10 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-7108-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 11 publications 11 publications

ELISA (ELISA)

View 4 publications 4 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES3-9D7

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834348

Product Specific Information

Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).

Applications Reported: This JES3-9D7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Histological changes of cervical tumours following Zanthoxylum acanthopodium DC treatment, and its impact on cytokine expression.

Saudi journal of biological sciences

Simanullang RH,Situmorang PC,Herlina M,Silalahi B,Manurung SS

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Immunohistochemistry

Fri Apr 01 00:00:00 EDT 2022

Circulating Regulatory B-Lymphocytes in Patients with Acute Myocardial Infarction: A Pilot Study.

Journal of cardiovascular development and disease

Volodarsky I,Shimoni S,Haberman D,Mirkin V,Fabrikant Y,Yoskovich Mashriki T,Zalik A,George J

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Thu Dec 22 00:00:00 EST 2022

Induction of cytotoxic effector cells towards cholangiocellular, pancreatic, and colorectal tumor cells by activation of the immune checkpoint CD40/CD40L on dendritic cells.

Cancer immunology, immunotherapy : CII

Sadeghlar F,Vogt A,Mohr RU,Mahn R,van Beekum K,Kornek M,Weismüller TJ,Branchi V,Matthaei H,Toma M,Schmidt-Wolf IGH,Kalff JC,Strassburg CP,González-Carmona MA

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in ELISA

Sat May 01 00:00:00 EDT 2021

Early loss of T lymphocyte 4-1BB receptor expression is associated with higher short-term mortality in alcoholic hepatitis.

PloS one

Eriksen LL,Nielsen MA,Laursen TL,Deleuran B,Vilstrup H,Støy S

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Mon Nov 29 00:00:00 EST 2021

Cysticercus cellulosae Regulates T-Cell Responses and Interacts With the Host Immune System by Excreting and Secreting Antigens.

Frontiers in cellular and infection microbiology

Fan X,Zhang Y,Ouyang R,Luo B,Li L,He W,Liu M,Jiang N,Yang F,Wang L,Zhou B

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

Altered T-Cell Subsets are Associated with Dysregulated Cytokine Secretion of CD4⁺ T Cells During HIV Infection.

Journal of inflammation research

Wang D,Jiang Y,Song Y,Zeng Y,Li C,Wang X,Liu Y,Xiao J,Kong Y,Zhao H

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Wed Apr 27 00:00:00 EDT 2022

CAIX Regulates GBM Motility and TAM Adhesion and Polarization through EGFR/STAT3 under Hypoxic Conditions.

International journal of molecular sciences

Huang BR,Liu YS,Lai SW,Lin HJ,Shen CK,Yang LY,Lu DY

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Fri Aug 14 00:00:00 EDT 2020

Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.

mSystems

Cekanaviciute E,Pröbstel AK,Thomann A,Runia TF,Casaccia P,Katz Sand I,Crabtree E,Singh S,Morrissey J,Barba P,Gomez R,Knight R,Mazmanian S,Graves J,Cree BAC,Zamvil SS,Baranzini SE

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

siRNA‑mediated knockdown of T‑bet and RORγt contributes to decreased inflammation in pre‑eclampsia.

Molecular medicine reports

Wang J,Wen ZQ,Cheng XY,Mei TY,Chen ZF,Su LX

53-7108-42 was used in Flow Cytometry to determine whether a decrease in the expression of T‚bet can restore immune balance and alleviate the systemic inflammatory response present in preeclampsia.

Wed Nov 01 00:00:00 EDT 2017

β-Catenin Stabilization in Skin Fibroblasts Causes Fibrotic Lesions by Preventing Adipocyte Differentiation of the Reticular Dermis.

The Journal of investigative dermatology

Mastrogiannaki M,Lichtenberger BM,Reimer A,Collins CA,Driskell RR,Watt FM

53-7108 was used in Flow cytometry/Cell sorting to study how Wnt/β-catenin signalling may control adipogenic cell fate within the lower dermis, which potentially contributes to the pathogenesis of fibrotic skin diseases.

Wed Jun 01 00:00:00 EDT 2016

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in ELISA

Thu Sep 01 00:00:00 EDT 2016

Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.

Laboratory investigation; a journal of technical methods and pathology

Mariuzzi L,Domenis R,Orsaria M,Marzinotto S,Londero AP,Bulfoni M,Candotti V,Zanello A,Ballico M,Mimmi MC,Calcagno A,Marchesoni D,Di Loreto C,Beltrami AP,Cesselli D,Gri G

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in ELISA

Thu Sep 01 00:00:00 EDT 2016

The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.

Oncology letters

Wang Y,Yi J,Chen X,Zhang Y,Xu M,Yang Z

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in ELISA

Mon Feb 01 00:00:00 EST 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using IL-10 monoclonal antibody (Product # 53-7108-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

CD4(+)CD25(-)Foxp3(+) T cells play a role in tuberculous hydrothorax rather than malignant hydrothorax.

Journal of translational medicine

Tang Y,Peng LP,Qin GX,Sun JT,Xu LJ,Jiang YF

53-7108 was used in Flow cytometry/Cell sorting to determine that CD4(+)CD25(-)Foxp3(+) T cells play a role in tuberculous hydrothorax rather than malignant hydrothorax.

Tue Aug 18 00:00:00 EDT 2015

Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.

Cellular immunology

Nowacki TM,Kuerten S,Zhang W,Shive CL,Kreher CR,Boehm BO,Lehmann PV,Tary-Lehmann M

Tue May 01 00:00:00 EDT 2007

Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.

Journal of immunology (Baltimore, Md. : 1950)

Sendide K,Deghmane AE,Pechkovsky D,Av-Gay Y,Talal A,Hmama Z

Sat Oct 15 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1; T-cell growth inhibitory factor

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Gene Aliases: CSIF; GVHDS; IL-10; IL10; IL10A; TGIF

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UniProt ID: (Human) P22301

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Entrez Gene ID: (Human) 3586

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Function(s)

cytokine activity interleukin-10 receptor binding protein binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (JES3-9D7), Alexa Fluor™ 488, eBioscience™

IL-10 Antibody (53-7108-42) in Flow

Product Details

53-7108-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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