Invitrogen

IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™

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IL-10 Antibody (11-7101-82) in Flow

Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0041-82) and 0.25 µg of Anti-Mouse IL-10 FITC.

IL-10 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

11-7101-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 34 publications 34 publications

ELISA (ELISA)

View 1 publication 1 publication

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES5-16E3

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_465403

Product Specific Information

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported:The JES5-16E3 antibody has been reported for use in capture of mouse IL-10 by ELISA and ELISPOT, intracellular staining for flow cytometric analysis, IHC, and neutralization of IL-10 bioactivity.

Applications Tested: Has been tested by intracellular staining with flow cytometric analysis of cultured mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2024

Myeloid-derived suppressor cells cross-talk with B10 cells by BAFF/BAFF-R pathway to promote immunosuppression in cervical cancer.

Cancer immunology, immunotherapy : CII

Jianyi D,Haili G,Bo Y,Meiqin Y,Baoyou H,Haoran H,Fang L,Qingliang Z,Lingfei H

11-7101-82 was used in Flow Cytometry to reveal a new mechanism of BAFF-mediated mutual immune regulation between MDSCs and B cells in the occurrence and development of cervical cancer.

Sun Jan 01 00:00:00 EST 2023

Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammation.

EMBO reports

Zhu K,Wang Y,Sarlus H,Geng K,Nutma E,Sun J,Kung SY,Bay C,Han J,Min JH,Benito-Cuesta I,Lund H,Amor S,Wang J,Zhang XM,Kutter C,Guerreiro-Cacais AO,Högberg B,Harris RA

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Jul 05 00:00:00 EDT 2022

RORγt phosphorylation protects against T cell-mediated inflammation.

Cell reports

Ma S,Patel SA,Abe Y,Chen N,Patel PR,Cho BS,Abbasi N,Zeng S,Schnabl B,Chang JT,Huang WJM

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2022

Peptide-siRNA nanoparticles targeting NF-κB p50 mitigate experimental abdominal aortic aneurysm progression and rupture.

Biomaterials advances

Yan H,Hu Y,Akk A,Wickline SA,Pan H,Pham CTN

11-7101-82 was used in flow cytometry to provide a rationale for selective targeting of p50 for more specific therapeutic intervention in the medical treatment of small AAA.

Mon Aug 01 00:00:00 EDT 2022

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Sat Nov 06 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

High Glucose Intake Exacerbates Autoimmunity through Reactive-Oxygen-Species-Mediated TGF-β Cytokine Activation.

Immunity

Zhang D,Jin W,Wu R,Li J,Park SA,Tu E,Zanvit P,Xu J,Liu O,Cain A,Chen W

11-7101-82 was used in Flow Cytometry to reveal a previously unrecognized mechanism underlying the adverse effects of high glucose intake in the pathogenesis of autoimmunity and inflammation.

Tue Oct 15 00:00:00 EDT 2019

Diabetes impairs wound healing by Dnmt1-dependent dysregulation of hematopoietic stem cells differentiation towards macrophages.

Nature communications

Yan J,Tie G,Wang S,Tutto A,DeMarco N,Khair L,Fazzio TG,Messina LM

11-7101 was used in Flow cytometry/Cell sorting to demonstrate for the first time that type 2 diabetes mellitus induces an haematopoietic stem cell-autonomous mechanism that causes impaired wound healing.

Tue Jan 02 00:00:00 EST 2018

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

EMBO molecular medicine

Huang SC,Wei PC,Hwang-Verslues WW,Kuo WH,Jeng YM,Hu CM,Shew JY,Huang CS,Chang KJ,Lee EY,Lee WH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Neutralization

Fri Dec 01 00:00:00 EST 2017

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Low-Dose Intestinal Trichuris muris Infection Alters the Lung Immune Microenvironment and Can Suppress Allergic Airway Inflammation.

Infection and immunity

Chenery AL,Antignano F,Burrows K,Scheer S,Perona-Wright G,Zaph C

11-7101 was used in Flow cytometry/Cell sorting to study crosstalk between the intestine and lung during disease, showing that low-dose intestinal Trichuris muris infection alters the lung immune microenvironment.

Mon Feb 01 00:00:00 EST 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner.

Cancer research

Kratochvill F,Gratz N,Qualls JE,Van De Velde LA,Chi H,Kovarik P,Murray PJ

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Dimethyl sulfoxide inhibits spontaneous diabetes and autoimmune recurrence in non-obese diabetic mice by inducing differentiation of regulatory T cells.

Toxicology and applied pharmacology

Lin GJ,Sytwu HK,Yu JC,Chen YW,Kuo YL,Yu CC,Chang HM,Chan DC,Huang SH

11-7101 was used in Flow cytometry/Cell sorting to demonstrate that in vivo DMSO treatment suppresses spontaneous diabetes and autoimmune recurrence in NOD mice.

Thu Jan 15 00:00:00 EST 2015

Preemptive donor apoptotic cell infusions induce IFN-γ-producing myeloid-derived suppressor cells for cardiac allograft protection.

Journal of immunology (Baltimore, Md. : 1950)

Bryant J,Lerret NM,Wang JJ,Kang HK,Tasch J,Zhang Z,Luo X

11-7101 was used in Flow cytometry/Cell sorting to investigate the role of MDSCs in the graft protection provided by ECDI-SPs, showing that preemptive donor apoptotic cell infusions induce IFN-γ-producing MDSCs.

Sun Jun 15 00:00:00 EDT 2014

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Prevention or early cure of type 1 diabetes by intranasal administration of gliadin in NOD mice.

PloS one

Funda DP,Fundova P,Hansen AK,Buschard K

11-7101 was used in Flow cytometry/Cell sorting to investigate whether intranasal (i.n.) administration of gliadin or gluten may arrest the diabetogenic process, showing that i.n. administration of gliadin prevents type 1 diabetes in NOD mice.

Tue Jan 13 00:00:00 EST 2015

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-10 monoclonal antibody (Product # 11-7101-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Infection rate and tissue localization of murine IL-12p40-producing monocyte-derived CD103(+) lung dendritic cells during pulmonary tuberculosis.

PloS one

Leepiyasakulchai C,Taher C,Chuquimia OD,Mazurek J,Söderberg-Naucler C,Fernández C,Sköld M

11-7101 was used in Flow cytometry/Cell sorting to demonstrate that CD103(+) dendritic cells in the lungs and pulmonary lymph nodes are monocyte-derived during pulmonary tuberculosis.

Tue Feb 11 00:00:00 EST 2014

Osteoclast activated FoxP3+ CD8+ T-cells suppress bone resorption in vitro.

PloS one

Buchwald ZS,Kiesel JR,DiPaolo R,Pagadala MS,Aurora R

11-7101 was used in Flow cytometry/Cell sorting to demonstrate the effects of activated CD8 T cell-produced cytokines on osteoclast activity.

Thu Nov 08 00:00:00 EST 2012

IL-10 regulates viral lung immunopathology during acute respiratory syncytial virus infection in mice.

PloS one

Loebbermann J,Schnoeller C,Thornton H,Durant L,Sweeney NP,Schuijs M,O'Garra A,Johansson C,Openshaw PJ

11-7101 was used in Flow cytometry/Cell sorting to study experimental respiratory syncytial virus infection in IL-10 deficient mice and mice treated with anti-IL-10 receptor antibody.

Mon Jul 23 00:00:00 EDT 2012

Infection with a helminth parasite attenuates autoimmunity through TGF-beta-mediated suppression of Th17 and Th1 responses.

Journal of immunology (Baltimore, Md. : 1950)

Walsh KP,Brady MT,Finlay CM,Boon L,Mills KH

11-7101 was used in Flow cytometry/Cell sorting to provide evidence that parasites can exert bystander immunosuppression of pathogenic T cells that mediate autoimmune diseases.

Sat Aug 01 00:00:00 EDT 2009

Ly49 C/I-dependent NKT cell-derived IL-10 is required for corneal graft survival and peripheral tolerance.

Journal of leukocyte biology

Watte CM,Nakamura T,Lau CH,Ortaldo JR,Stein-Streilein J

11-7101 was used in Flow cytometry/Cell sorting to test if the NK inhibitory receptor functionally contributed to tolerance development.

Tue Apr 01 00:00:00 EDT 2008

Deletion of both ICAM-1 and C3 enhances severity of experimental autoimmune encephalomyelitis compared to C3-deficient mice.

Neuroscience letters

Smith SS,Ludwig M,Wohler JE,Bullard DC,Szalai AJ,Barnum SR

11-7101 was used in Flow cytometry/Cell sorting to examine experimental autoimmune encephalitis in ICAM-1 x C3 double knockout mice.

Fri Sep 12 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

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Gene Aliases: CSIF; Il-10; Il10

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UniProt ID: (Mouse) P18893

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Entrez Gene ID: (Mouse) 16153

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Function(s)

cytokine activity interleukin-10 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-10 Monoclonal Antibody (JES5-16E3), FITC, eBioscience™

IL-10 Antibody (11-7101-82) in Flow

Product Details

11-7101-82

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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