Invitrogen

IL-10 Monoclonal Antibody (JES5-16E3), PerCP-Cyanine5.5, eBioscience™

FIGURE: 1 / 27

IL-10 Antibody (45-7101-82) in Flow

Intracellular staining of Mouse Cytokine Positive Control Cells (Product # 00-4500-51) with Anti-Mouse CD4 PE (Product # 12-0042-82) and 0.25 µg of Rat IgG2b K Isotype Control PerCP-Cyanine5-5 (Product # 45-4031-80) (left) or 0.25 µg of Anti-Mouse IL-10 PerCP-Cyanine5-5 (right).

Product Image Gallery

Product Details

45-7101-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 24 publications 24 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES5-16E3

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_996677

Product Specific Information

Description: The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported: This JES5-16E3 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This JES5-16E3 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Fasudil-modified macrophages reduce inflammation and regulate the immune response in experimental autoimmune encephalomyelitis.

Neural regeneration research

Liu C,Guo S,Liu R,Guo M,Wang Q,Chai Z,Xiao B,Ma C

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2024

Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis.

iScience

Zhou J,Wang H,Shu T,Wang J,Yang W,Li J,Ding L,Liu M,Sun H,Wong J,Lai PB,Tsang SW,Ward SE,Chow KL,Sung JJ,Sze-Lok Cheng A

45-7101-82 was used in ELISA to demonstrated that cell cycle-related kinase (CCRK) is upregulated in myeloid cells in tumor-bearing mice and in patients with hepatocellular carcinoma.

Fri Oct 20 00:00:00 EDT 2023

Myeloid cell-specific topoisomerase 1 inhibition using DNA origami mitigates neuroinflammation.

EMBO reports

Zhu K,Wang Y,Sarlus H,Geng K,Nutma E,Sun J,Kung SY,Bay C,Han J,Min JH,Benito-Cuesta I,Lund H,Amor S,Wang J,Zhang XM,Kutter C,Guerreiro-Cacais AO,Högberg B,Harris RA

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Tue Jul 05 00:00:00 EDT 2022

RORγt phosphorylation protects against T cell-mediated inflammation.

Cell reports

Ma S,Patel SA,Abe Y,Chen N,Patel PR,Cho BS,Abbasi N,Zeng S,Schnabl B,Chang JT,Huang WJM

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Tue Mar 15 00:00:00 EDT 2022

Gut epithelial IL-27 confers intestinal immunity through the induction of intraepithelial lymphocytes.

The Journal of experimental medicine

Lin CH,Chen MC,Lin LL,Christian DA,Min B,Hunter CA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Irradiated whole-cell vaccine suppresses hepatocellular carcinoma growth in mice via Th9 cells.

Oncology letters

Chen J,Ding Y,Huang F,Lan R,Wang Z,Huang W,Chen R,Wu B,Fu L,Yang Y,Liu J,Hong J,Zhang W,Zhang L

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.

Theranostics

Tong L,Hao H,Zhang Z,Lv Y,Liang X,Liu Q,Liu T,Gong P,Zhang L,Cao F,Pastorin G,Lee CN,Chen X,Wang JW,Yi H

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Fri Jan 28 00:00:00 EST 2022

Characterisation of Macrophage Polarisation in Mice Infected with Ninoa Strain of Trypanosoma cruzi.

Pathogens (Basel, Switzerland)

Medina-Buelvas DM,Rodríguez-Sosa M,Vega L

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Sat Nov 06 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

IL-13 Ameliorates Neuroinflammation and Promotes Functional Recovery after Traumatic Brain Injury.

Journal of immunology (Baltimore, Md. : 1950)

Miao W,Zhao Y,Huang Y,Chen D,Luo C,Su W,Gao Y

45-7101 was used in Flow cytometry/Cell sorting to demonstrate that IL-13 treatment improved neurologic outcomes after Traumatic Brain Injury through adjusting microglia/macrophage phenotypes and inhibiting inflammatory responses.

Sun Mar 15 00:00:00 EDT 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice.

JCI insight

Cai W,Dai X,Chen J,Zhao J,Xu M,Zhang L,Yang B,Zhang W,Rocha M,Nakao T,Kofler J,Shi Y,Stetler RA,Hu X,Chen J

45-7101 was used in Flow cytometry/Cell sorting to suggest that efferocytosis via STAT6/Arg1 modulates microglia/macrophage phenotype, accelerates inflammation resolution, and improves stroke outcomes.

Thu Oct 17 00:00:00 EDT 2019

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Maternal-Derived Hepatitis B Virus e Antigen Alters Macrophage Function in Offspring to Drive Viral Persistence after Vertical Transmission.

Immunity

Tian Y,Kuo CF,Akbari O,Ou JH

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Tue May 17 00:00:00 EDT 2016

Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.

PloS one

Singh S,Vedi S,Samrat SK,Li W,Kumar R,Agrawal B

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Tue Jul 19 00:00:00 EDT 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

Towards programming immune tolerance through geometric manipulation of phosphatidylserine.

Biomaterials

Roberts RA,Eitas TK,Byrne JD,Johnson BM,Short PJ,McKinnon KP,Reisdorf S,Luft JC,DeSimone JM,Ting JP

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Tristetraprolin Limits Inflammatory Cytokine Production in Tumor-Associated Macrophages in an mRNA Decay-Independent Manner.

Cancer research

Kratochvill F,Gratz N,Qualls JE,Van De Velde LA,Chi H,Kovarik P,Murray PJ

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

Mouse bone marrow-derived mesenchymal stem cells inhibit leukemia/lymphoma cell proliferation in vitro and in a mouse model of allogeneic bone marrow transplant.

International journal of molecular medicine

Song N,Gao L,Qiu H,Huang C,Cheng H,Zhou H,Lv S,Chen L,Wang J

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

A reproducible method for the expansion of mouse CD8+ T lymphocytes.

Journal of immunological methods

Lewis MD,de Leenheer E,Fishman S,Siew LK,Gross G,Wong FS

45-7101 was used in Flow cytometry/Cell sorting to report a tissue culture protocol for the reliable expansion of CD8+ T-cells derived from murine spleens.

Sun Feb 01 00:00:00 EST 2015

Nizatidine, a small molecular compound, enhances killed H5N1 vaccine cell-mediated responses and protects mice from lethal viral challenge.

Human vaccines & immunotherapeutics

Wang S,Wu B,Xue J,Wang M,Chen R,Wang B

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Mon Jan 05 00:00:00 EST 2015

BCG vaccine mediated reduction in the MHC-II expression of macrophages and dendritic cells is reversed by activation of Toll-like receptors 7 and 9.

Cellular immunology

Bakhru P,Sirisaengtaksin N,Soudani E,Mukherjee S,Khan A,Jagannath C

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-10 monoclonal antibody (Product # 45-7101-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

ICAM-1 deficiency exacerbates sarcoid-like granulomatosis induced by Propionibacterium acnes through impaired IL-10 production by regulatory T cells.

The American journal of pathology

Kamata M,Tada Y,Mitsui A,Shibata S,Miyagaki T,Asano Y,Sugaya M,Kadono T,Sato S

45-7101 was used in Flow cytometry/Cell sorting to show that intercellular adhesion molecule-1 is imperative for inducing regulatory T cells to produce IL-10 in vivo, which prevents granuloma formation.

Sun Dec 01 00:00:00 EST 2013

Bioinformatics

Protein Aliases: CSIF; Cytokine synthesis inhibitory factor; H-IL-10; il 10; IL-10; ILN; Interleukin; Interleukin-10; Interleukin10; MGC126450; MGC126451; RP11-262N9.1

View more View less

Gene Aliases: CSIF; Il-10; Il10

View more View less

UniProt ID: (Mouse) P18893

View more View less

Entrez Gene ID: (Mouse) 16153

View more View less

Function(s)

cytokine activity interleukin-10 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-10 Monoclonal Antibody (JES5-16E3), PerCP-Cyanine5.5, eBioscience™

IL-10 Antibody (45-7101-82) in Flow

Product Details

45-7101-82

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!