Invitrogen

IL-12 p70 Monoclonal Antibody (C17-8), Biotin

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Datasheet

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Tech Support

Product Details

MM121B

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

View 14 publications 14 publications

Neutralization (Neu)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

C17-8

Immunogen

Recombinant mouse IL-12

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223600

Product Specific Information

MM121B targets IL-12 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM121B immunogen is recombinant mouse IL-12.

MM121B detects IL-12 which has a predicted molecular weight of approximately 22 kDa.

The MM121B mouse IL12 antibody (biotinylated conjugate of clone C17-8) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM120 (clone 9A5). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody MM121B (clone C17-8) and antibody MM120 (clone 9A5) have successfully been used in combination with recombinant mouse IL12p70 protein SMIL12 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

IL-12 p70, also known as interleukin-12, is a cytokine that plays a critical role in the immune system's response to infections and cancer. It is produced by immune cells, including dendritic cells, macrophages, and B cells, in response to certain pathogens and tumor cells. IL-12 p70 stimulates the production of interferon-gamma and other cytokines, which promote the activation and proliferation of T cells and natural killer cells. IL-12 p70 has been studied as a potential therapeutic target for various diseases and conditions, including infectious diseases, autoimmune disorders, and cancer. It has been shown to enhance immune responses against certain infections and tumors, and to modulate inflammatory responses in autoimmune diseases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A flagellin-conjugate protein induces dual NLRC4- and NLRP3-inflammasome activation which modulates inflammatory cytokine secretion from macrophages.

Frontiers in immunology

Lin YJ,Jamin A,Wolfheimer S,Fiedler A,Junker AC,Goretzki A,Scheurer S,Schülke S

MM121B was used in ELISA to investigate the mechanisms underlying the production of IL-1β from rFlaA:Betv1 stimulated macrophages.

Mon Apr 10 00:00:00 EDT 2023

The Fusion Protein rFlaA:Betv1 Modulates DC Responses by a p38-MAPK and COX2-Dependent Secretion of PGE₂ from Epithelial Cells.

Cells

Lin YJ,Flaczyk A,Wolfheimer S,Goretzki A,Jamin A,Wangorsch A,Vieths S,Scheurer S,Schülke S

MM121B was used in Enzyme-linked immunosorbent assay to investigate the immune-modulatory effects of rFlaA:Betv1 on airway epithelial cells. LA-4 mouse lung epithelial cells were stimulated with rFlaA:Betv1 in the presence/absence of various inhibitors with cytokine- and chemokine secretion quantified by ELISA and activation of intracellular signaling cascades demonstrated by Western blot (WB).

Sat Dec 04 00:00:00 EST 2021

Inhibition of Inflammatory Gene Transcription by IL-10 Is Associated with Rapid Suppression of Lipopolysaccharide-Induced Enhancer Activation.

Journal of immunology (Baltimore, Md. : 1950)

Conaway EA,de Oliveira DC,McInnis CM,Snapper SB,Horwitz BH

MM121B was used in an ELISA assay to demonstrate that inhibition of transcription is the primary mechanism for IL-10-mediated suppression in LPS-stimulated macrophages and that inhibited genes can be divided into two clusters.

Sat Apr 01 00:00:00 EDT 2017

A polymorphism in the coding region of Il12b promotes IL-12p70 and IL-23 heterodimer formation.

Journal of immunology (Baltimore, Md. : 1950)

Zwiers A,Fuss IJ,Seegers D,Konijn T,Garcia-Vallejo JJ,Samsom JN,Strober W,Kraal G,Bouma G

MM121B was used in western blot to investigate the effect of a polymorphism in the coding region of Il12b for interleukin-12p70 and interleukin-23 heterodimer formation

Tue Mar 15 00:00:00 EDT 2011

Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes.

Infection and immunity

Dewamitta SR,Nomura T,Kawamura I,Hara H,Tsuchiya K,Kurenuma T,Shen Y,Daim S,Yamamoto T,Qu H,Sakai S,Xu Y,Mitsuyama M

MM121B was used in ELISA to investigate the role of listeriolysin O in the calpain activation and interleukin-1 alpha secretion in Listeria monocytogene-infected macrophages

Sat May 01 00:00:00 EDT 2010

1-Methylnicotinamide and nicotinamide: two related anti-inflammatory agents that differentially affect the functions of activated macrophages.

Archivum immunologiae et therapiae experimentalis

Biedroń R,Ciszek M,Tokarczyk M,Bobek M,Kurnyta M,Słominska EM,Smoleński RT,Marcinkiewicz J

MM121B was used in ELISA to study the effects of 1-methylnicotinamide and nicotinamide on the generation of inflammatory mediators by macrophages

Tue May 20 00:00:00 EDT 2008

Heligmosomoides polygyrus promotes regulatory T-cell cytokine production in the murine normal distal intestine.

Infection and immunity

Setiawan T,Metwali A,Blum AM,Ince MN,Urban JF,Elliott DE,Weinstock JV

MM121B was used in ELISA to investigate the effect of exposure to Heligmosomoides polygyrus on cytokine pathways in murine normal distal intestine

Sat Sep 01 00:00:00 EDT 2007

The influence of collagenase treatment on the production of TNF-alpha, IL-6 and IL-10 by testicular macrophages.

Journal of immunological methods

Bryniarski K,Szczepanik M,Ptak M,Ptak W

MM121B was used in ELISA to study the effect of collagenase treatment on cytokine production by testicular macrophages

Wed Jun 01 00:00:00 EDT 2005

Modulation of testicular macrophage activity by collagenase.

Folia histochemica et cytobiologica

Bryniarski K,Szczepanik M,Ptak M,Ptak W

MM121B was used in ELISA to investigate the regulation of cytokines and the expression of ROI in testicular macrophages

Tue Aug 09 00:00:00 EDT 2005

Activated natural killer cell-mediated immunity is required for the inhibition of tumor metastasis by dendritic cell vaccination.

Experimental & molecular medicine

Kim A,Noh YW,Kim KD,Jang YS,Choe YK,Lim JS

MM121B was used in blocking/activating experiment to investigate the effect of dendritic cell vaccination for the prevention of tumor migration

Sun Oct 31 00:00:00 EDT 2004

Heligmosomoides polygyrus inhibits established colitis in IL-10-deficient mice.

European journal of immunology

Elliott DE,Setiawan T,Metwali A,Blum A,Urban JF,Weinstock JV

MM121B was used in ELISA to investigate the effect of Heligmosomoides polygyrus on colitis

Fri Oct 01 00:00:00 EDT 2004

In vivo compartmentalization of functionally distinct, rapidly responsive antigen-specific T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice.

Infection and immunity

Kirby AC,Sundquist M,Wick MJ

MM121B was used in blocking or activating experiment to characterize specific memory T-cell populations in DNA-immunized or Salmonella enterica serovar Typhimurium-infected mice

Mon Nov 01 00:00:00 EST 2004

NF-kappa B is required within the innate immune system to inhibit microflora-induced colitis and expression of IL-12 p40.

Journal of immunology (Baltimore, Md. : 1950)

Tomczak MF,Erdman SE,Poutahidis T,Rogers AB,Holcombe H,Plank B,Fox JG,Horwitz BH

MM121B was used in ELISA to study the role of NF-kappa B and the innate immune system in inhibiting microflora-induced colitis

Fri Aug 01 00:00:00 EDT 2003

Modulation of macrophage activity by proteolytic enzymes. Differential regulation of IL-6 and reactive oxygen intermediates (ROIs) synthesis as a possible homeostatic mechanism in the control of inflammation.

Inflammation

Bryniarski K,Maresz K,Szczepanik M,Ptak M,Ptak W

MM121B was used in ELISA to investigate the effect of collagenase and trypsin for the regulation of macrophage function

Mon Dec 01 00:00:00 EST 2003

Quantities of interleukin-12p40 in mature CD8alpha negative dendritic cells correlate with strength of TCR signal and determine Th cell development.

Microbiology and immunology

Fujimoto N,Ishida H,Nakamura I,Ogasawara K,Itoh Y

MM121B was used in ELISA and flow cytometry to report that the strength of TCR signaling directs Th cell development by regulating CD40L expression on T cells which determines production of IL-12p40 from DC via CD40.

Fri Mar 05 00:00:00 EST 2004

Interleukin 12 and antigen independently induce substance P receptor expression in T cells in murine schistosomiasis mansoni.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Blum AM,Metwali A,Crawford C,Li J,Qadir K,Elliott DE,Weinstock JV

MM121B was used in ELISA to investigate the promoting effect of interleukin 12 and schistosome egg antigen on substance P receptor expression

Sun Apr 01 00:00:00 EST 2001

CD40 ligation prevents neonatal induction of transplantation tolerance.

Journal of immunology (Baltimore, Md. : 1950)

Flamand V,Donckier V,Demoor FX,Le Moine A,Matthys P,Vanderhaeghen ML,Tagawa Y,Iwakura Y,Billiau A,Abramowicz D,Goldman M

MM121B was used in blocking/activating experiment to study the effect of CD40 ligation on neonatal transplantation tolerance

Fri May 15 00:00:00 EDT 1998

Differential stimulation requirements for IL-12 production among clones of macrophage hybridomas.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Taneichi M,Horino A,Naito S,Sakurai S,Komuro K,Uchida T

MM121B was used in ELISA to investigate the effect of interferon gamma and LPS on interleukin 12 production from macrophage hybridomas

Wed Oct 01 00:00:00 EDT 1997

Bioinformatics

Protein Aliases: 20C2; CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12p70; IL-12 p75; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL12A/B; IL12AB; ILN; Interleukin; Interleukin-12 p70; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; MGC151228; MGC151232

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; Il12a; Il12b; Il12p40; Ll12a; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160

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Function(s)

transmembrane signal receptor

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-12 p70 Monoclonal Antibody (C17-8), Biotin

Product Details

MM121B

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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