Invitrogen

IL-13 Monoclonal Antibody (eBio13A), APC-eFluor™ 780, eBioscience™

FIGURE: 1 / 15

IL-13 Antibody (47-7133-82) in Flow

BALB/c splenocytes were stimulated for 3 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82), soluble Anti-Mouse CD28 Functional Grade Purified (Product # 16-0281-82), Mouse IL-2 Recombinant Protein (Product # 14-8021-64), and Mouse IL-4 Recombinant Protein (Product # 14-8041-80). Cells were then restimulated with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) for 5 hours. Following restimulation, cells were fixed and permeabilized (Product # 88-8824-00) and then stai... View More

IL-13 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

IL-13 Antibody in Flow Cytometry, ELISA (Flow, ELISA)

Product Image Gallery

Product Details

47-7133-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 14 publications 14 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio13A

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2716964

Product Specific Information

Description: The eBio13A antibody reacts with mouse IL-13. IL-13 is a cytokine produced mainly by Th2 cells, but also by antigen-primed CD8 T cells. IL-13 has a strong involvement in allergic inflammation and parasitic clearing. In cancer models it has been shown to have either inhibitory or stimulatory activity depending on the tumor. In humans, IL-13 is found to play a role in isotype switching in B cells. IL-13 is implicating in down modulating macrophage activity, through the reduction of pro-inflammatory cytokines (IL-1, IL-6, IL-8, IL-10, IL-12)

Applications Reported: This eBio13A antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio13A antibody has been tested by intracellular staining and flow cytometric analysis of Th2-polarized mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and is involved in regulating inflammatory and immune responses. IL-13 down-regulates macrophage activity, thereby inhibiting the production of pro-inflammatory cytokines and chemokines, and is involved in allergen-induced asthma through mechanisms independent of IgE and eosinophils. IL-13 also regulates several stages of B cell maturation and differentiation, up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. Structurally, IL-13 closely resembles IL-3, IL-4, IL-5 and GM-CSF. The gene encoding IL-13, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with the IL-13 gene particularly close to IL4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Self-amplifying mRNA seasonal influenza vaccines elicit mouse neutralizing antibody and cell-mediated immunity and protect ferrets.

NPJ vaccines

Cheung M,Chang C,Rathnasinghe R,Rossignol E,Zhang Y,Ferrari A,Patel H,Huang Y,Sanchez Guillen M,Scalzo T,Lee C,Otten GR,Settembre EC,Music N,Palladino G,Wen Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Wed Oct 04 00:00:00 EDT 2023

Monitoring Group 2 Innate Lymphoid Cell Biology in Models of Lung Inflammation.

Bio-protocol

Badrani JH,Strohm AN,Haung YA,Doherty TA

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Jul 20 00:00:00 EDT 2023

Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.

Nature communications

Cheong LY,Wang B,Wang Q,Jin L,Kwok KHM,Wu X,Shu L,Lin H,Chung SK,Cheng KKY,Hoo RLC,Xu A

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Mitochondrial transcription factor A in RORγt⁺ lymphocytes regulate small intestine homeostasis and metabolism.

Nature communications

Fu Z,Dean JW,Xiong L,Dougherty MW,Oliff KN,Chen ZE,Jobin C,Garrett TJ,Zhou L

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Jul 22 00:00:00 EDT 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in ELISA

Fri Oct 22 00:00:00 EDT 2021

Synergistic effect of non-neutralizing antibodies and interferon-γ for cross-protection against influenza.

iScience

Shibuya M,Tamiya S,Kawai A,Hirai T,Cragg MS,Yoshioka Y

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in ELISA

Fri Oct 22 00:00:00 EDT 2021

Type 1 Cytotoxic T Cells Increase in Placenta after Intrauterine Inflammation.

Frontiers in immunology

Liu J,Liu Y,Panda S,Liu A,Lei J,Burd I

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Fri Dec 17 00:00:00 EST 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Gut Microbial Metabolite Pravastatin Attenuates Intestinal Ischemia/Reperfusion Injury Through Promoting IL-13 Release From Type II Innate Lymphoid Cells via IL-33/ST2 Signaling.

Frontiers in immunology

Deng F,Hu JJ,Yang X,Sun QS,Lin ZB,Zhao BC,Yao ZW,Luo SD,Chen ZL,Liu Y,Yan ZZ,Li C,Liu WF,Liu KX

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

6-Phosphogluconate dehydrogenase (6PGD), a key checkpoint in reprogramming of regulatory T cells metabolism and function.

eLife

Daneshmandi S,Cassel T,Higashi RM,Fan TW,Seth P

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Thu Oct 28 00:00:00 EDT 2021

ILC3s control airway inflammation by limiting T cell responses to allergens and microbes.

Cell reports

Teng F,Tachó-Piñot R,Sung B,Farber DL,Worgall S,Hammad H,Lambrecht BN,Hepworth MR,Sonnenberg GF

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma.

Nature immunology

Harb H,Stephen-Victor E,Crestani E,Benamar M,Massoud A,Cui Y,Charbonnier LM,Arbag S,Baris S,Cunnigham A,Leyva-Castillo JM,Geha RS,Mousavi AJ,Guennewig B,Schmitz-Abe K,Sioutas C,Phipatanakul W,Chatila TA

47-7133 was used in Flow cytometry/Cell sorting to find that interleukin-6- and STAT3 transcription factor-dependent upregulation of Notch4 receptor on lung tissue regulatory T (Treg) cells is necessary for allergens and particulate matter pollutants to promote airway inflammation.

Sun Nov 01 00:00:00 EDT 2020

A helminth-derived suppressor of ST2 blocks allergic responses.

eLife

Vacca F,Chauché C,Jamwal A,Hinchy EC,Heieis G,Webster H,Ogunkanbi A,Sekne Z,Gregory WF,Wear M,Perona-Wright G,Higgins MK,Nys JA,Cohen ES,McSorley HJ

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Tue Dec 24 00:00:00 EST 2019

Nematode-Infected Mice Acquire Resistance to Subsequent Infection With Unrelated Nematode by Inducing Highly Responsive Group 2 Innate Lymphoid Cells in the Lung.

Frontiers in immunology

Yasuda K,Adachi T,Koida A,Nakanishi K

Published figure using IL-13 monoclonal antibody (Product # 47-7133-82) in Flow Cytometry

Mon Sep 30 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: ALRH; ALRH antibody; BHR1; il 13; IL-13; ILN; Interleukin; Interleukin-13; Interleukin13; MGC116786; MGC116788; MGC116789; NC30 antibody; P600 antibody; T-cell activation protein P600

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Gene Aliases: Il-13; Il13

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UniProt ID: (Mouse) P20109

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Entrez Gene ID: (Mouse) 16163

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Function(s)

cytokine activity cytokine receptor binding interleukin-13 receptor binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-13 Monoclonal Antibody (eBio13A), APC-eFluor™ 780, eBioscience™

IL-13 Antibody (47-7133-82) in Flow

Product Details

47-7133-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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