Invitrogen

IL-17A Monoclonal Antibody (eBio64DEC17), APC, eBioscience™

IL-17A Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 1

IL-17A Antibody (17-7179-42) in Flow

CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23 Recombinant Protein (Product # 14-8239-63) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (left) or Cell Stimulation Cocktail plus protein transport inhibitors (Product # 00-4975-03) (right) for 6 hours. Cells were intracellularly stained with Anti-Human CD4 FITC (Product # 11-0047-42) and Anti-Human IL-17A APC using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-882... View More

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Product Details

17-7179-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 27 publications 27 publications

Functional Assay (Functional)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Fungi, Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio64DEC17

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1582221

Product Specific Information

Description: The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies.

Applications Reported: This eBio64DEC17 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio64DEC17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Post-transplant cyclophosphamide prevents xenogeneic graft-versus-host disease while depleting proliferating regulatory T cells.

iScience

Ritacco C,Köse MC,Courtois J,Canti L,Beguin C,Dubois S,Vandenhove B,Servais S,Caers J,Beguin Y,Ehx G,Baron F

17-7179-42 was used in Flow cytometry/Cell sorting to demonstrated that highly xenoreactive T-cell clones were depleted by PTCy. Although Treg frequencies were significantly higher in PTCy-treated than in control mice on day 21, xGVHD attenuation by PTCy was not abrogated by Treg depletion.

Fri Mar 17 00:00:00 EDT 2023

Fungal sensing by dectin-1 directs the non-pathogenic polarization of T_H17 cells through balanced type I IFN responses in human DCs.

Nature immunology

Gringhuis SI,Kaptein TM,Remmerswaal EBM,Drewniak A,Wevers BA,Theelen B,D'Haens GRAM,Boekhout T,Geijtenbeek TBH

17-7179-42 was used in Flow Cytometry /test) to show that dectin-1-mediated modulation of type I IFN responses allowed TGF-β activation and non-pathogenic TH17 cell development during fungal infections in humans.

Thu Dec 01 00:00:00 EST 2022

Chronic morphine administration differentially modulates viral reservoirs in SIVmac251 infected rhesus macaque model.

Journal of virology

Acharya A,Olwenyi OA,Thurman M,Pandey K,Morsey BM,Lamberty B,Ferguson N,Callen S,Fang Q,Buch SJ,Fox HS,Byrareddy SN

17-7179-42 was used in Flow Cytometry to suggest that people with HIV who abuse opioids had higher reservoirs in CNS than the lymphoid system.

Mon Mar 01 00:00:00 EST 2021

Single-cell RNA sequencing identifies shared differentiation paths of mouse thymic innate T cells.

Nature communications

Lee M,Lee E,Han SK,Choi YH,Kwon DI,Choi H,Lee K,Park ES,Rha MS,Joo DJ,Shin EC,Kim S,Kim JK,Lee YJ

17-7179 was used in Flow cytometry/Cell sorting to indicate that innate T cells share effector differentiation processes in the thymus.

Mon Aug 31 00:00:00 EDT 2020

Tumors induce de novo steroid biosynthesis in T cells to evade immunity.

Nature communications

Mahata B,Pramanik J,van der Weyden L,Polanski K,Kar G,Riedel A,Chen X,Fonseca NA,Kundu K,Campos LS,Ryder E,Duddy G,Walczak I,Okkenhaug K,Adams DJ,Shields JD,Teichmann SA

17-7179 was used in Flow cytometry/Cell sorting to show that tumors induce de novo steroidogenesis in T lymphocytes to evade anti-tumor immunity.

Fri Jul 17 00:00:00 EDT 2020

Respiratory Syncytial Virus Infection Reduces Kynurenic Acid Production and Reverses Th17/Treg Balance by Modulating Indoleamine 2,3-Dioxygenase (IDO) Molecules in Plasmacytoid Dendritic Cells.

Medical science monitor : international medical journal of experimental and clinical research

Jin L,Hu Q,Hu Y,Chen Z,Liao W

17-7179 was used in Flow cytometry/Cell sorting to understand that respiratory syncytial virus infection reduces the production of kynurenic acid and inhibits transformation from Th17 to Foxp3+ regulatory T cells by modulating indoleamine 2,3-dioxygenase molecules in plasmacytoid dendritic cells.

Wed Dec 02 00:00:00 EST 2020

IFN-γ promoted exosomes from mesenchymal stem cells to attenuate colitis via miR-125a and miR-125b.

Cell death & disease

Yang R,Huang H,Cui S,Zhou Y,Zhang T,Zhou Y

17-7179-42 was used in Flow Cytometry to investigate the potential anti-inflammatory effects of bone marrow mesenchymal stem cell-derived exosomes.

Thu Jul 30 00:00:00 EDT 2020

Interleukin-24 Regulates T Cell Activity in Patients With Colorectal Adenocarcinoma.

Frontiers in oncology

Zhang Y,Liu Y,Xu Y

17-7179-42 was used in Flow Cytometry to indicate that IL-24 might regulate T cell function in a dose-dependent manner.

Wed Sep 30 00:00:00 EDT 2020

Two phase kinetics of the inflammatory response from hepatocyte-peripheral blood mononuclear cell interactions.

Scientific reports

Beringer A,Molle J,Bartosch B,Miossec P

17-7179 was used in Flow cytometry/Cell sorting to study the role of interactions between non and activated human peripheral blood mononuclear cells and human hepatoma HepaRG cells in the inflammatory response.

Mon Jun 10 00:00:00 EDT 2019

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

17-7179 was used in Flow cytometry/Cell sorting to indicate that CD4 T-cell deficiency, Th2 and Th17 bias were closely correlated with the severity of SFTS, indicating therapeutic potential of early immune interventions to ameliorate disease severity.

Mon Oct 01 00:00:00 EDT 2018

The microvascular niche instructs T cells in large vessel vasculitis via the VEGF-Jagged1-Notch pathway.

Science translational medicine

Wen Z,Shen Y,Berry G,Shahram F,Li Y,Watanabe R,Liao YJ,Goronzy JJ,Weyand CM

17-7179 was used in Flow cytometry/Cell sorting to study the VEGF-Jagged1-Notch pathway and the effects of T cells in pathogenic large vessel vasculitis.

Wed Jul 19 00:00:00 EDT 2017

Antigen-Presenting Human γδ T Cells Promote Intestinal CD4⁺ T Cell Expression of IL-22 and Mucosal Release of Calprotectin.

Journal of immunology (Baltimore, Md. : 1950)

Tyler CJ,McCarthy NE,Lindsay JO,Stagg AJ,Moser B,Eberl M

17-7179 was used in Flow cytometry/Cell sorting to study the role of γδ T cells in the regulation of IL-22 expression.

Mon May 01 00:00:00 EDT 2017

Azacytidine prevents experimental xenogeneic graft-versus-host disease without abrogating graft-versus-leukemia effects.

Oncoimmunology

Ehx G,Fransolet G,de Leval L,D'Hondt S,Lucas S,Hannon M,Delens L,Dubois S,Drion P,Beguin Y,Humblet-Baron S,Baron F

17-7179 was used in Flow cytometry/Cell sorting to investigate the impact of 5-azacytidine on xenogeneic graft-vs.-host disease and graft-vs.-leukaemia effects in a humanised murine model of transplantation.

Tue Jan 12 00:00:00 EST 2021

High frequency of intestinal T_H17 cells correlates with microbiota alterations and disease activity in multiple sclerosis.

Science advances

Cosorich I,Dalla-Costa G,Sorini C,Ferrarese R,Messina MJ,Dolpady J,Radice E,Mariani A,Testoni PA,Canducci F,Comi G,Martinelli V,Falcone M

17-7179 was used in Flow cytometry/Cell sorting to assess the crucial role of the intestinal environment in promoting Th17 cell expansion in human multiple sclerosis patients.

Sat Jul 01 00:00:00 EDT 2017

Role of podoplanin in the high interleukin-17A secretion resulting from interactions between activated lymphocytes and psoriatic skin-derived mesenchymal cells.

Clinical and experimental immunology

Noack M,Ndongo-Thiam N,Miossec P

17-7179-42 was used in Flow cytometry/Cell sorting to study the interactions of skin-derived fibroblasts with peripheral blood mononuclear cells (PBMC) with a focus on the Th17 pathway and to identify a mechanism which leads to a high interleukin (IL)-17 secretion.

Sat Oct 01 00:00:00 EDT 2016

Reduced pro-inflammatory profile of γδT cells in pregnant patients with rheumatoid arthritis.

Arthritis research & therapy

Tham M,Schlör GR,Yerly D,Mueller C,Surbek D,Villiger PM,Förger F

17-7179 was used in Flow cytometry/Cell sorting to investigate whether pregnancy-related changes in disease activity were associated with changes in the proportion and function of γδT cells.

Fri Jan 22 00:00:00 EST 2016

European journal of immunology

Shen H,Wang Y,Chen CY,Frencher J,Huang D,Yang E,Ryan-Payseur B,Chen ZW

17-7179 was used in Flow cytometry/Cell sorting to examine the involvement of cytokines in the adaptive immune response to bacterial infection.

Sun Feb 01 00:00:00 EST 2015

Administration of Panobinostat Is Associated with Increased IL-17A mRNA in the Intestinal Epithelium of HIV-1 Patients.

Mediators of inflammation

Bjerg Christensen A,Dige A,Vad-Nielsen J,Brinkmann CR,Bendix M,Østergaard L,Tolstrup M,Søgaard OS,Rasmussen TA,Randel Nyengaard J,Agnholt J,Denton PW

17-7179 was used in Flow cytometry/Cell sorting to investigate the effects of new therapeutic strategies for HIV treatment in the intestines.

Mon Sep 26 00:00:00 EDT 2016

The Clinical Relevance of IL-17-Producing CD4+CD161+ Cell and Its Subpopulations in Primary Sjögren's Syndrome.

Journal of immunology research

Li L,He J,Zhu L,Yang Y,Jin Y,Jia R,Liu X,Liu Y,Sun X,Li Z

17-7179 was used in Flow cytometry/Cell sorting to assess the contribution of CD4+CD161+ T cell populations to the development of Sjögren's syndrome.

Tue May 24 00:00:00 EDT 2016

Polycyclic aromatic hydrocarbons reciprocally regulate IL-22 and IL-17 cytokines in peripheral blood mononuclear cells from both healthy and asthmatic subjects.

PloS one

Plé C,Fan Y,Ait Yahia S,Vorng H,Everaere L,Chenivesse C,Balsamelli J,Azzaoui I,de Nadai P,Wallaert B,Lazennec G,Tsicopoulos A

17-7179 was used in Flow cytometry/Cell sorting to investigate whether PAH affects production of IL-17 and IL-22 production in allergic asthma, showing they reciprocally regulate IL-22 and IL-17 cytokines in peripheral blood mononuclear cells.

Thu Mar 31 00:00:00 EDT 2016

Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection.

PLoS pathogens

Li H,Richert-Spuhler LE,Evans TI,Gillis J,Connole M,Estes JD,Keele BF,Klatt NR,Reeves RK

17-7179-42 was used in Flow cytometry/Cell sorting to demonstrate the first evidence for acute, systemic, and permanent loss of mucosal innate lymphoid cells during SIV infection associated with reduction of IL-17.

Mon Dec 01 00:00:00 EST 2014

Vitamin D up-regulates the vitamin D receptor by protecting it from proteasomal degradation in human CD4+ T cells.

PloS one

Kongsbak M,von Essen MR,Boding L,Levring TB,Schjerling P,Lauritsen JP,Woetmann A,Ødum N,Bonefeld CM,Geisler C

17-7179 was used in Flow cytometry/Cell sorting to determine if and how 1,25(OH)2D3 by itself regulates vitamin D receptor (VDR) expression in human CD4+ T cells.

Thu Jun 04 00:00:00 EDT 2015

Elevated levels of CD4(+)CD25(+)FoxP3(+) T cells in systemic sclerosis patients contribute to the secretion of IL-17 and immunosuppression dysfunction.

PloS one

Liu X,Gao N,Li M,Xu D,Hou Y,Wang Q,Zhang G,Sun Q,Zhang H,Zeng X

17-7179 was used in Flow cytometry/Cell sorting to investigate the role of Treg subsets in the immune imbalance in naive systemic sclerosis (SSc), showing that elevated levels of CD4(+)CD25(+)FoxP3(+) T cells contribute to the secretion of IL-17 and immunosuppression dysfunction.

Thu Jan 16 00:00:00 EST 2014

Peripherally circulating CD4⁺ FOXP3⁺ CXCR3⁺ T regulatory cells correlate with renal allograft function.

Scandinavian journal of immunology

Hoerning A,Köhler S,Jun C,Tebbe B,Fu J,Menke J,Wilde B,Dolff S,Feldkamp T,Briscoe DM,Kribben A,Hoyer PF,Witzke O

17-7179 was used in Flow cytometry/Cell sorting to investigate the relevance of trafficking receptors for Tregs after solid organ transplantation.

Sat Sep 01 00:00:00 EDT 2012

Tumour cell generation of inducible regulatory T-cells in multiple myeloma is contact-dependent and antigen-presenting cell-independent.

PloS one

Feyler S,Scott GB,Parrish C,Jarmin S,Evans P,Short M,McKinley K,Selby PJ,Cook G

17-7179 was used in Immunofluorescence to provide further evidence of direct tumour manipulation of the immune system to augment immune evasion and propagation of the malignant cell clone.

Mon Oct 29 00:00:00 EDT 2012

IL-21 regulates the differentiation of a human γδ T cell subset equipped with B cell helper activity.

PloS one

Caccamo N,Todaro M,La Manna MP,Sireci G,Stassi G,Dieli F

17-7179 was used in Flow cytometry/Cell sorting to characterise a follicular B-helper T cell subset formed when Vγ9Vδ2 T lymphocytes are cocultured with phosphoantigen and IL-21.

Thu Jan 10 00:00:00 EST 2013

Differentiation, phenotype, and function of interleukin-17-producing human Vγ9Vδ2 T cells.

Blood

Caccamo N,La Mendola C,Orlando V,Meraviglia S,Todaro M,Stassi G,Sireci G,Fournié JJ,Dieli F

17-7179 was used in Flow cytometry/Cell sorting to describe a new subset of human IL-17(+) Vγ9Vδ2 T lymphocytes implicated in the inflammatory responses during bacterial infections.

Thu Jul 07 00:00:00 EDT 2011

Prostaglandin E2 and IL-23 plus IL-1β differentially regulate the Th1/Th17 immune response of human CD161(+) CD4(+) memory T cells.

Clinical and translational science

Barrie A,Khare A,Henkel M,Zhang Y,Barmada MM,Duerr R,Ray A

17-7179 was used in Flow cytometry/Cell sorting to suggest that Th17-mediated chronic inflammatory bowel disease depends on a threshold response of CD161(+) CD4(+) memory T cells to both PGE2 and IL-23 plus IL-1β.

Mon Aug 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A

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Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A

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UniProt ID: (Human) Q16552

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Entrez Gene ID: (Human) 3605

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Function(s)

cytokine activity cytokine receptor binding protein binding

Process(es)

apoptotic process inflammatory response immune response cell surface receptor signaling pathway cell-cell signaling cell death positive regulation of necrotic cell death positive regulation of interleukin-23 production response to amino acid positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter defense response to fungus cellular response to interleukin-1 cellular response to glucocorticoid stimulus fibroblast activation positive regulation of cytokine production involved in inflammatory response positive regulation of interleukin-6 secretion

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IL-17A Monoclonal Antibody (eBio64DEC17), APC, eBioscience™

IL-17A Antibody (17-7179-42) in Flow

Product Details

17-7179-42

Flow Cytometry (Flow)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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