Invitrogen

IL-17A Monoclonal Antibody (eBio64DEC17), APC-eFluor™ 780, eBioscience™

IL-17A Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 34

IL-17A Antibody (47-7179-42) in Flow

CD4-enriched human peripheral blood cells were polarized under Th17 conditions (with Human IL-23 Recombinant Protein (Product # 14-8239-63) for 10 days. Cells were restimulated with Protein Transport Inhibitor Cocktail (Product # 00-4980-03) (left) or Cell Stimulation Cocktail plus protein transport inhibitors (Product # 00-4975-03) (right) for 6 hours. Cells were stained intracellularly with Anti-Human CD4 FITC (Product # 11-0047-42) and Anti-Human IL-17A APC-eFluor® 780 using the Intracellular Fixation & Permeabilization Buffer Set (Prod... View More

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Product Details

47-7179-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 26 publications 26 publications

Product Specifications

Species Reactivity

Human

Published species

Hamster, Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio64DEC17

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11043559

Product Specific Information

Description: The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13, an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.

IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN gamma and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.

Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies.

Applications Reported: This eBio64DEC17 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio64DEC17 antibody has been pre-titrated and tested by intracellular flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-17A (IL-17A, CTLA-8) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. IL-17A is a 32 kDa long, disulfide-linked homodimer consisting of 136 amino acids that is a member of a six-species family of proteins (IL-17A-17F) and signals through the IL-17 receptor (IL-17R/CDw217). High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells, and IL-17A also enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. In particular, human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A regulates the activities of NF-kappa B and mitogen-activated protein kinases, stimulates the expression of IL-6 and cyclooxygenase-2 (PTGS2/COX-2), and enhances the production of nitric oxide (NO).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Antigen-Specific Intraocular Cytokine Responses Distinguish Ocular Tuberculosis From Undifferentiated Uveitis in Tuberculosis-Immunoreactive Patients.

American journal of ophthalmology

Alam K,Sharma G,Forrester JV,Basu S

47-7179-42 was used in Flow cytometry/Cell sorting to show that the intraocular polyfunctional cytokine response is stronger in undifferentiated TST+ uveitis than in OTB patients, likely representing an exaggerated anti-TB immune response rather than active infection.

Wed Feb 01 00:00:00 EST 2023

Identification of aryl hydrocarbon receptor as a barrier to HIV-1 infection and outgrowth in CD4⁺ T cells.

Cell reports

Chatterjee D,Zhang Y,Ngassaki-Yoka CD,Dutilleul A,Khalfi S,Hernalsteens O,Wiche Salinas TR,Dias J,Chen H,Smail Y,Goulet JP,Bell B,Routy JP,Van Lint C,Ancuta P

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Tue Jun 27 00:00:00 EDT 2023

Cathepsin S inhibition in dendritic cells prevents Th17 cell differentiation in perivascular adipose tissues following vascular injury in diabetic rats.

Journal of biochemical and molecular toxicology

Peng H,Lv Y,Li C,Cheng Z,He S,Wang C,Liu J

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Engineered Human Dendritic Cell Exosomes as Effective Delivery System for Immune Modulation.

International journal of molecular sciences

Elsayed R,Elashiry M,Tran C,Yang T,Carroll A,Liu Y,Hamrick M,Cutler CW

47-7179-42 was used in Flow cytometry/Cell sorting to report in a mouse model of periodontitis that custom murine DC-derived exo subtypes could reprogram the immune response toward a bone-sparing or bone-loss phenotype, depending on immune profile.

Tue Jul 11 00:00:00 EDT 2023

FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.

Molecular therapy. Nucleic acids

Chang GP,Yang XL,Liu W,Lin S,Yang SL,Zhao MY

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Small molecule allosteric inhibitors of RORγt block Th17-dependent inflammation and associated gene expression in vivo.

PloS one

Saenz SA,Local A,Carr T,Shakya A,Koul S,Hu H,Chourb L,Stedman J,Malley J,D'Agostino LA,Shanmugasundaram V,Malona J,Schwartz CE,Beebe L,Clements M,Rajaraman G,Cho J,Jiang L,Dubrovskiy A,Kreilein M,Shimanovich R,Hamann LG,Escoubet L,Ellis JM

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Wed Dec 29 00:00:00 EST 2021

YY1 regulation by miR-124-3p promotes Th17 cell pathogenicity through interaction with T-bet in rheumatoid arthritis.

JCI insight

Lin J,Tang J,Lin J,He Y,Yu Z,Jiang R,Yang B,Ou Q

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Mon Nov 22 00:00:00 EST 2021

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

Developing Human Skin Contains Lymphocytes Demonstrating a Memory Signature.

Cell reports. Medicine

Dhariwala MO,Karthikeyan D,Vasquez KS,Farhat S,Weckel A,Taravati K,Leitner EG,Clancy S,Pauli M,Piper ML,Cohen JN,Ashouri JF,Lowe MM,Rosenblum MD,Scharschmidt TC

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Tue Nov 17 00:00:00 EST 2020

HIV vaccine candidate activation of hypoxia and the inflammasome in CD14⁺ monocytes is associated with a decreased risk of SIV_mac251 acquisition.

Nature medicine

Vaccari M,Fourati S,Gordon SN,Brown DR,Bissa M,Schifanella L,Silva de Castro I,Doster MN,Galli V,Omsland M,Fujikawa D,Gorini G,Liyanage NPM,Trinh HV,McKinnon KM,Foulds KE,Keele BF,Roederer M,Koup RA,Shen X,Tomaras GD,Wong MP,Munoz KJ,Gach JS,Forthal DN,Montefiori DC,Venzon DJ,Felber BK,Rosati M,Pavlakis GN,Rao M,Sekaly RP,Franchini G

47-7179 was used in Flow cytometry/Cell sorting to examine the ability of several vaccine prime modalities together with two ALVAC-SIV + gp120 protein boosts to reduce the risk of SIVmac251 acquisition in Rhesus macaques.

Fri Jun 01 00:00:00 EDT 2018

CXCL4 is a novel inducer of human Th17 cells and correlates with IL-17 and IL-22 in psoriatic arthritis.

European journal of immunology

Affandi AJ,Silva-Cardoso SC,Garcia S,Leijten EFA,van Kempen TS,Marut W,van Roon JAG,Radstake TRDJ

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Accumulation of T-helper 22 cells, interleukin-22 and myeloid-derived suppressor cells promotes gastric cancer progression in elderly patients.

Oncology letters

Chen X,Wang Y,Wang J,Wen J,Jia X,Wang X,Zhang H

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2018

Enhancing the toolbox to study IL-17A in cattle and sheep.

Veterinary research

Wattegedera SR,Corripio-Miyar Y,Pang Y,Frew D,McNeilly TN,Palarea-Albaladejo J,McInnes CJ,Hope JC,Glass EJ,Entrican G

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Sat Apr 08 00:00:00 EDT 2017

Berberine exerts an anti-inflammatory role in ocular Behcet's disease.

Molecular medicine reports

Yang Y,Wang Q,Xie M,Liu P,Qi X,Liu X,Li Z

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Sun Jan 01 00:00:00 EST 2017

Transcriptional signature of human pro-inflammatory T_H17 cells identifies reduced IL10 gene expression in multiple sclerosis.

Nature communications

Hu D,Notarbartolo S,Croonenborghs T,Patel B,Cialic R,Yang TH,Aschenbrenner D,Andersson KM,Gattorno M,Pham M,Kivisakk P,Pierre IV,Lee Y,Kiani K,Bokarewa M,Tjon E,Pochet N,Sallusto F,Kuchroo VK,Weiner HL

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Fri Nov 17 00:00:00 EST 2017

Single-cell profiling reveals GPCR heterogeneity and functional patterning during neuroinflammation.

JCI insight

Tischner D,Grimm M,Kaur H,Staudenraus D,Carvalho J,Looso M,Günther S,Wanke F,Moos S,Siller N,Breuer J,Schwab N,Zipp F,Waisman A,Kurschus FC,Offermanns S,Wettschureck N

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Thu Aug 03 00:00:00 EDT 2017

Human uterine lymphocytes acquire a more experienced and tolerogenic phenotype during pregnancy.

Scientific reports

Feyaerts D,Benner M,van Cranenbroek B,van der Heijden OWH,Joosten I,van der Molen RG

47-7179 was used in Flow cytometry/Cell sorting to immunophenotype lymphocytes from endometrium, term decidua parietalis, and peripheral blood mononuclear cells for direct comparison.

Tue Jun 06 00:00:00 EDT 2017

Surrogate in vitro activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens.

Oncotarget

Pathangey LB,McCurry DB,Gendler SJ,Dominguez AL,Gorman JE,Pathangey G,Mihalik LA,Dang Y,Disis ML,Cohen PA

47-7179 was used in Flow cytometry/Cell sorting to create a mechanistically rational culture sequence, enabling rapid preparation of T-cells recognizing tumor-associated antigens expressed by the majority of human cancer.

Tue Feb 14 00:00:00 EST 2017

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Thu Jun 23 00:00:00 EDT 2016

Interleukin-25 Mediates Transcriptional Control of PD-L1 via STAT3 in Multipotent Human Mesenchymal Stromal Cells (hMSCs) to Suppress Th17 Responses.

Stem cell reports

Wang WB,Yen ML,Liu KJ,Hsu PJ,Lin MH,Chen PM,Sudhir PR,Chen CH,Chen CH,Sytwu HK,Yen BL

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

IL32 is progressively expressed in mycosis fungoides independent of helper T-cell 2 and helper T-cell 9 polarization.

Cancer immunology research

Ohmatsu H,Humme D,Gulati N,Gonzalez J,Möbs M,Suárez-Fariñas M,Cardinale I,Mitsui H,Guttman-Yassky E,Sterry W,Krueger JG

47-7179 was used in Flow cytometry/Cell sorting to reveal a potential, unique role for IL32 in mycosis fungoides progression as an autocrine cytokine.

Mon Sep 01 00:00:00 EDT 2014

An ACT1 mutation selectively abolishes interleukin-17 responses in humans with chronic mucocutaneous candidiasis.

Immunity

Boisson B,Wang C,Pedergnana V,Wu L,Cypowyj S,Rybojad M,Belkadi A,Picard C,Abel L,Fieschi C,Puel A,Li X,Casanova JL

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Thu Oct 17 00:00:00 EDT 2013

Increased frequencies of Th22 cells as well as Th17 cells in the peripheral blood of patients with ankylosing spondylitis and rheumatoid arthritis.

PloS one

Zhang L,Li YG,Li YH,Qi L,Liu XG,Yuan CZ,Hu NW,Ma DX,Li ZF,Yang Q,Li W,Li JM

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Fri Jul 27 00:00:00 EDT 2012

Gain-of-function human STAT1 mutations impair IL-17 immunity and underlie chronic mucocutaneous candidiasis.

The Journal of experimental medicine

Liu L,Okada S,Kong XF,Kreins AY,Cypowyj S,Abhyankar A,Toubiana J,Itan Y,Audry M,Nitschke P,Masson C,Toth B,Flatot J,Migaud M,Chrabieh M,Kochetkov T,Bolze A,Borghesi A,Toulon A,Hiller J,Eyerich S,Eyerich K,Gulácsy V,Chernyshova L,Chernyshov V,Bondarenko A,Grimaldo RM,Blancas-Galicia L,Beas IM,Roesler J,Magdorf K,Engelhard D,Thumerelle C,Burgel PR,Hoernes M,Drexel B,Seger R,Kusuma T,Jansson AF,Sawalle-Belohradsky J,Belohradsky B,Jouanguy E,Bustamante J,Bué M,Karin N,Wildbaum G,Bodemer C,Lortholary O,Fischer A,Blanche S,Al-Muhsen S,Reichenbach J,Kobayashi M,Rosales FE,Lozano CT,Kilic SS,Oleastro M,Etzioni A,Traidl-Hoffmann C,Renner ED,Abel L,Picard C,Maródi L,Boisson-Dupuis S,Puel A,Casanova JL

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2011

Interleukin-12 is the optimum cytokine to expand human Th17 cells in vitro.

Clinical and vaccine immunology : CVI

Nady S,Ignatz-Hoover J,Shata MT

Published figure using IL-17A monoclonal antibody (Product # 47-7179-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CTLA-8; Cytotoxic T-lymphocyte-associated antigen 8; cytotoxic T-lymphocyte-associated protein 8; il 17; IL-17; ILN; Interleukin; interleukin 17; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); Interleukin-17A; Interleukin17A; R-IL-17-A

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Gene Aliases: CTLA-8; CTLA8; IL-17; IL-17A; IL17; IL17A

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UniProt ID: (Human) Q16552

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Entrez Gene ID: (Human) 3605

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Function(s)

cytokine activity cytokine receptor binding protein binding

Process(es)

apoptotic process inflammatory response immune response cell surface receptor signaling pathway cell-cell signaling cell death positive regulation of necrotic cell death positive regulation of interleukin-23 production response to amino acid positive regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter defense response to fungus cellular response to interleukin-1 cellular response to glucocorticoid stimulus fibroblast activation positive regulation of cytokine production involved in inflammatory response positive regulation of interleukin-6 secretion

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IL-17A Monoclonal Antibody (eBio64DEC17), APC-eFluor™ 780, eBioscience™

IL-17A Antibody (47-7179-42) in Flow

Product Details

47-7179-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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