Invitrogen

IL-2 Monoclonal Antibody (JES6-1A12), Functional Grade, eBioscience™

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IL-2 Antibody (16-7022-81) in IS

Splenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in Mouse IL-2 ELISPOT assay. Control well is medium alone.

IL-2 Antibody in Immunocytochemistry (ICC/IF)

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Product Details

16-7022-81

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

View 1 publication 1 publication

ELISA (ELISA)

View 5 publications 5 publications

Immunoprecipitation (IP)

Assay-Dependent

Neutralization (Neu)

Assay-Dependent

View 1 publication 1 publication

Functional Assay (Functional)

Assay-Dependent

View 8 publications 8 publications

Immunostaining (IS)

0.250 µg/mL

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

JES6-1A12

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469206

Product Specific Information

Description: The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.

Applications Reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, and IP.

Applications Tested: The Functional Grade Purified JES6-1A12 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-2 bioactivity.

The JES6-1A12 antibody at 0.250 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-2 in a CTLL-2 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.

The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Deletion Timing of Cic Alleles during Hematopoiesis Determines the Degree of Peripheral CD4⁺ T Cell Activation and Proliferation.

Immune network

Park GY,Lee GW,Kim S,Hong H,Park JS,Cho JH,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 16-7022-81) in ELISA

Thu Oct 01 00:00:00 EDT 2020

UCP3 reciprocally controls CD4+ Th17 and Treg cell differentiation.

PloS one

O'Connor EB,Muñoz-Wolf N,Leon G,Lavelle EC,Mills KHG,Walsh PT,Porter RK

16-7022 was used in Flow cytometry/Cell sorting to demonstrate that Ucp3 is expressed in peripheral naive CD4+ T cells at the mRNA level before being markedly downregulated following activation.

Thu Jan 07 00:00:00 EST 2021

A Stat6/Pten Axis Links Regulatory T Cells with Adipose Tissue Function.

Cell metabolism

Kälin S,Becker M,Ott VB,Serr I,Hosp F,Mollah MMH,Keipert S,Lamp D,Rohner-Jeanrenaud F,Flynn VK,Scherm MG,Nascimento LFR,Gerlach K,Popp V,Dietzen S,Bopp T,Krishnamurthy P,Kaplan MH,Serrano M,Woods SC,Tripal P,Palmisano R,Jastroch M,Blüher M,Wolfrum C,Weigmann B,Ziegler AG,Mann M,Tschöp MH,Daniel C

16-7022-81 was used in In Vivo experiments to study the link between adipose tissue function and regulatory T cells.

Tue Sep 05 00:00:00 EDT 2017

Capicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5.

Nature communications

Park S,Lee S,Lee CG,Park GY,Hong H,Lee JS,Kim YM,Lee SB,Hwang D,Choi YS,Fryer JD,Im SH,Lee SW,Lee Y

Published figure using IL-2 monoclonal antibody (Product # 16-7022-81) in ELISA

Wed Jul 12 00:00:00 EDT 2017

Thymic regulatory T cell niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition.

Nature immunology

Weist BM,Kurd N,Boussier J,Chan SW,Robey EA

16-7022 was used in Functional assays to investigate the sources of thymic IL-2 that limit access to the Treg developmental niche, showing that niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition.

Mon Jun 01 00:00:00 EDT 2015

Combination Therapy Using IL-2/IL-2 Monoclonal Antibody Complexes, Rapamycin, and Islet Autoantigen Peptides Increases Regulatory T Cell Frequency and Protects against Spontaneous and Induced Type 1 Diabetes in Nonobese Diabetic Mice.

Journal of immunology (Baltimore, Md. : 1950)

Manirarora JN,Wei CH

16-7022-81 was used in Functional assay to investigate whether expanded antigen-specific regulatory T cells can prevent or delay the development of type 1 diabetes in the NOD mouse model.

Tue Dec 01 00:00:00 EST 2015

Analysis of the vaccine potential of plasmid DNA encoding nine mycolactone polyketide synthase domains in Mycobacterium ulcerans infected mice.

PLoS neglected tropical diseases

Roupie V,Pidot SJ,Einarsdottir T,Van Den Poel C,Jurion F,Stinear TP,Huygen K

Published figure using IL-2 monoclonal antibody (Product # 16-7022-81) in ELISA

Mon Sep 08 00:00:00 EDT 2014

Evaluation of immunosuppressive function of regulatory T cells using a novel in vitro cytotoxicity assay.

Cell & bioscience

Zhang L,Manirarora JN,Wei CH

16-7022 was used in Functional assays to investigate the roles of regulatory T cells in treating various immune-mediated disorders.

Fri Apr 24 00:00:00 EDT 2015

The systemic immune state of super-shedder mice is characterized by a unique neutrophil-dependent blunting of TH1 responses.

PLoS pathogens

Gopinath S,Hotson A,Johns J,Nolan G,Monack D

Fri Jan 17 00:00:00 EST 2014

The systemic immune state of super-shedder mice is characterized by a unique neutrophil-dependent blunting of TH1 responses.

PLoS pathogens

Gopinath S,Hotson A,Johns J,Nolan G,Monack D

Fri Jan 17 00:00:00 EST 2014

GATA3 controls Foxp3⁺ regulatory T cell fate during inflammation in mice.

The Journal of clinical investigation

Wohlfert EA,Grainger JR,Bouladoux N,Konkel JE,Oldenhove G,Ribeiro CH,Hall JA,Yagi R,Naik S,Bhairavabhotla R,Paul WE,Bosselut R,Wei G,Zhao K,Oukka M,Zhu J,Belkaid Y

16-7022 was used in Functional assays to show that the canonical Th2 transcription factor GATA3 is selectively expressed in Tregs residing in barrier sites.

Tue Nov 01 00:00:00 EDT 2011

TNF optimally activatives regulatory T cells by inducing TNF receptor superfamily members TNFR2, 4-1BB and OX40.

European journal of immunology

Hamano R,Huang J,Yoshimura T,Oppenheim JJ,Chen X

16-7022 was used in Neutralization experiments to investigate pleiotropy in TNF inflammatory effects, showing that it activates Tregs by inducing TNFR2, 4-1BB and OX40.

Fri Jul 01 00:00:00 EDT 2011

Expansion of regulatory T cells via IL-2/anti-IL-2 mAb complexes suppresses experimental myasthenia.

European journal of immunology

Liu R,Zhou Q,La Cava A,Campagnolo DI,Van Kaer L,Shi FD

16-7022 was used in Functional assays to show that injection of cytokine IL-2 and anti-IL-2 mAb immune complexes induces effective and sustained expansion of Tregs.

Tue Jun 01 00:00:00 EDT 2010

Decrease of Foxp3+ Treg cell number and acquisition of effector cell phenotype during lethal infection.

Immunity

Oldenhove G,Bouladoux N,Wohlfert EA,Hall JA,Chou D,Dos Santos L,O'Brien S,Blank R,Lamb E,Natarajan S,Kastenmayer R,Hunter C,Grigg ME,Belkaid Y

16-7022 was used in Immunofluorescence to reveal a mechanism for Th1 cell pathogenicity that extends beyond their proinflammatory program to limit Treg cell survival.

Fri Nov 20 00:00:00 EST 2009

Modulation of pulmonary dendritic cell function during mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Liu Y,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 16-7022-81) in ELISA

Fri Feb 01 00:00:00 EST 2008

Modulation of naive CD4+ T-cell responses to an airway antigen during pulmonary mycobacterial infection.

Infection and immunity

Anis MM,Fulton SA,Reba SM,Harding CV,Boom WH

Published figure using IL-2 monoclonal antibody (Product # 16-7022-81) in ELISA

Tue May 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: H-IL-2; il 2; IL-2; ILN; Interleukin; Interleukin-2; Interleukin2; Involved in regulation of T cell clonal expansion; M-IL-2; T-cell growth factor; T-cell growth factor (TCGF); TCGF

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Gene Aliases: Il-2; Il2

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UniProt ID: (Mouse) P04351

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Entrez Gene ID: (Mouse) 16183

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Function(s)

cytokine activity interleukin-2 receptor binding growth factor activity carbohydrate binding kappa-type opioid receptor binding glycosphingolipid binding actin or actin-binding cytoskeletal protein non-receptor serine/threonine protein kinase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-2 Monoclonal Antibody (JES6-1A12), Functional Grade, eBioscience™

IL-2 Antibody (16-7022-81) in IS

Product Details

16-7022-81

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Functional Assay (Functional)

Immunostaining (IS)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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