Invitrogen

IL-4 Monoclonal Antibody (8D4-8), PE, eBioscience™

FIGURE: 1 / 13

IL-4 Antibody (12-7049-42) in Flow

Normal human peripheral blood cells were stimulated with PMA and Ionomycin in the presence of brefeldin A for 6 hours. The cells were surface stained with Anti-Human CD3 FITC (Product # 11-0038-42) and intracellularly stained with Anti-Human IL-4 PE (right panel). Left panel demonstrates unlabelled antibody blocking control.

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Product Details

12-7049-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 24 publications 24 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Nematode

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

8D4-8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1548817

Product Specific Information

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported:The 8D4-8 antibody has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining for flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested by intracellular staining for flow cytometric analysis. This can be used at test size: 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using IL-4 monoclonal antibody (Product # 12-7049-42) in Flow Cytometry

Wed Feb 23 00:00:00 EST 2022

Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively.

International journal of molecular sciences

Perveen K,Quach A,McPhee A,Prescott SL,Barry SC,Hii CS,Ferrante A

12-7049-42 was used in Flow Cytometry to support the view that low CBTC PKCζ levels relate to the increased risk of developing allergic diseases.

Wed May 05 00:00:00 EDT 2021

Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively.

International journal of molecular sciences

Perveen K,Quach A,McPhee A,Prescott SL,Barry SC,Hii CS,Ferrante A

12-7049-42 was used in Flow Cytometry to support the view that low CBTC PKCζ levels relate to the increased risk of developing allergic diseases.

Wed May 05 00:00:00 EDT 2021

Recombinant human IL-37 inhibited endometriosis development in a mouse model through increasing Th1/Th2 ratio by inducing the maturation of dendritic cells.

Reproductive biology and endocrinology : RB&E

Li L,Liao Z,Ye M,Jiang J

12-7049-42 was used in Flow cytometry/Cell sorting to study recombinant human IL-37 (rhIL-37) is an inhibitor of inflammation.

Tue Aug 24 00:00:00 EDT 2021

Single-cell multi-omics analysis presents the landscape of peripheral blood T-cell subsets in human chronic prostatitis/chronic pelvic pain syndrome.

Journal of cellular and molecular medicine

Zhang M,Liu Y,Chen J,Chen L,Meng J,Yang C,Yin S,Zhang X,Zhang L,Hao Z,Chen X,Liang C

12-7049 was used in Flow cytometry/Cell sorting to digitally quantify both the protein and mRNA expression levels in single peripheral blood T cells from two chronic prostatitis / chronic pelvic pain patients and two healthy controls.

Tue Dec 01 00:00:00 EST 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using IL-4 monoclonal antibody (Product # 12-7049-42) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using IL-4 monoclonal antibody (Product # 12-7049-42) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

CD4⁺ T_h immunogenicity of the Ascaris spp. secreted products.

NPJ vaccines

Ebner F,Morrison E,Bertazzon M,Midha A,Hartmann S,Freund C,Álvaro-Benito M

12-7049-42 was used in Flow Cytometry to identify the first human T cell epitope against Ascaris spp. and represents an easily adaptable platform for characterization of complex antigens.

Thu Mar 18 00:00:00 EDT 2021

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using IL-4 monoclonal antibody (Product # 12-7049-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia.

Oncology letters

Di N,Guo Y,Ding N

12-7049-42 was used in Flow cytometry/Cell sorting to investigate the effect of combined propofol-sevoflurane anesthesia on immune function in pediatric patients with acute lymphoblastic leukemia (ALL).

Mon Jul 01 00:00:00 EDT 2019

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

12-7049 was used in Flow cytometry/Cell sorting to indicate that CD4 T-cell deficiency, Th2 and Th17 bias were closely correlated with the severity of SFTS, indicating therapeutic potential of early immune interventions to ameliorate disease severity.

Mon Oct 01 00:00:00 EDT 2018

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

Mon Oct 01 00:00:00 EDT 2018

Lactobacillus rhamnosus GG-Derived Soluble Mediators Modulate Adaptive Immune Cells.

Frontiers in immunology

Ludwig IS,Broere F,Manurung S,Lambers TT,van der Zee R,van Eden W

Tue Sep 26 00:00:00 EDT 2023

Genetic Architecture of Adaptive Immune System Identifies Key Immune Regulators.

Cell reports

Lagou V,Garcia-Perez JE,Smets I,Van Horebeek L,Vandebergh M,Chen L,Mallants K,Prezzemolo T,Hilven K,Humblet-Baron S,Moisse M,Van Damme P,Boeckxstaens G,Bowness P,Dubois B,Dooley J,Liston A,Goris A

12-7049-42 was used in Flow Cytometry to identify eight genome-wide significant associations explaining 6%-20% of variance in immune system regulation.

Tue Oct 16 00:00:00 EDT 2018

Functionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells.

International journal of nanomedicine

Tomić S,Ilić N,Kokol V,Gruden-Movsesijan A,Mihajlović D,Bekić M,Sofronić-Milosavljević L,Čolić M,Vučević D

12-7049 was used in Flow cytometry/Cell sorting to investigate how differentially functionalised cellulose nanofibrils affect dendritic cell properties, their viability, morphology, differentiation and maturation potential, and the capacity to regulate T cell-mediated immune response.

Mon Dec 31 00:00:00 EST 2018

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

12-7049 was used in Flow cytometry/Cell sorting to investigate the function of enhancer of zeste homolog 2 in human Th1 and Th2 cell differentiation, and its association with allergic rhinitis.

Wed Nov 20 00:00:00 EST 2019

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

12-7049 was used in Flow cytometry/Cell sorting to investigate the function of enhancer of zeste homolog 2 in human Th1 and Th2 cell differentiation, and its association with allergic rhinitis.

Wed Nov 20 00:00:00 EST 2019

Mucosal and systemic immune modulation by Trichuris trichiura in a self-infected individual.

Parasite immunology

Dige A,Rasmussen TK,Nejsum P,Hagemann-Madsen R,Williams AR,Agnholt J,Dahlerup JF,Hvas CL

12-7049-42 was used in Flow cytometry/Cell sorting to investigated how self-infection with Trichuris (T.) trichiura in an adult man without intestinal disease affected mucosal and systemic immunity.

Sun Jan 01 00:00:00 EST 2017

A pilot study showing associations between frequency of CD4(+) memory cell subsets at diagnosis and duration of partial remission in type 1 diabetes.

Clinical immunology (Orlando, Fla.)

Moya R,Robertson HK,Payne D,Narsale A,Koziol J,Davies JD

12-7049 was used in Flow cytometry/Cell sorting to assess whether frequencies of CD4(+) T cell subsets in children newly diagnosed with type 1 diabetes are associated with length of partial remission.

Sun May 01 00:00:00 EDT 2016

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

12-7049 was used in Flow cytometry/Cell sorting to demonstrate that CD16+monocytes were enriched and played a critical role in driving the pathogenic T- and B-cell responses in patients with systemic lupus erythematosus.

Wed Nov 20 00:00:00 EST 2019

Effects of transcutaneous acupoint electrical stimulation on the imbalance of Th₁, Th₂, Th₁₇ and T_reg cells following thoracotomy of patients with lung cancer.

Experimental and therapeutic medicine

Wu H,Wang K,Li G,Meng D,Han J,Wang G,Li YU

12-7049 was used in Flow cytometry/Cell sorting to demonstrate that transcutaneous acupoint electrical stimulation was able to partially attenuate the postoperative immune depression of patients with lung cancer.

Mon Feb 01 00:00:00 EST 2016

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Nucleic acids research

Zhong F,Zhou N,Wu K,Guo Y,Tan W,Zhang H,Zhang X,Geng G,Pan T,Luo H,Zhang Y,Xu Z,Liu J,Liu B,Gao W,Liu C,Ren L,Li J,Zhou J,Zhang H

Published figure using IL-4 monoclonal antibody (Product # 12-7049-42) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2015

T-helper 17 cell polarization in pulmonary arterial hypertension.

Chest

Hautefort A,Girerd B,Montani D,Cohen-Kaminsky S,Price L,Lambrecht BN,Humbert M,Perros F

12-7049 was used in Flow cytometry/Cell sorting to study T helper 17 cell immune polarization in patients with pulmonary arterial hypertension.

Mon Jun 01 00:00:00 EDT 2015

Do patients with a failed metal-on-metal hip implant with a pseudotumor present differences in their peripheral blood lymphocyte subpopulations?

Clinical orthopaedics and related research

Catelas I,Lehoux EA,Hurda I,Baskey SJ,Gala L,Foster R,Kim PR,Beaulé PE

12-7049 was used in Flow cytometry/Cell sorting to reveal the presence of a type IV hypersensitivity reaction in patients with a failed hip implant with a pseudotumour.

Tue Dec 01 00:00:00 EST 2015

Depressive symptoms post hip fracture in older adults are associated with phenotypic and functional alterations in T cells.

Immunity & ageing : I & A

Duggal NA,Upton J,Phillips AC,Hampson P,Lord JM

12-7049 was used in Flow cytometry/Cell sorting to investigate the synergistic effects of hip fracture physical trauma and psychological depressive symptoms on the aged immune system.

Wed Jan 28 00:00:00 EST 2015

BET bromodomain inhibition suppresses TH17-mediated pathology.

The Journal of experimental medicine

Mele DA,Salmeron A,Ghosh S,Huang HR,Bryant BM,Lora JM

12-7049 was used in Flow cytometry/Cell sorting to identify BET proteins as a fundamental link between chromatin signalling and T(H)17 biology.

Mon Oct 21 00:00:00 EDT 2013

Brefeldin A, but not monensin, enables flow cytometric detection of interleukin-4 within peripheral T cells responding to ex vivo stimulation with Chlamydia trachomatis.

Journal of immunological methods

Vicetti Miguel RD,Maryak SA,Cherpes TL

12-7049 was used in Flow cytometry/Cell sorting to compare the ability of brefeldin A and monensin to concurrently trap 4 cytokines within peripheral blood mononuclear cells.

Wed Oct 31 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)

cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (8D4-8), PE, eBioscience™

IL-4 Antibody (12-7049-42) in Flow

Product Details

12-7049-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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