Invitrogen

IL-4 Monoclonal Antibody (8D4-8), APC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

11 Published Figures

18 References

View all (109) IL-4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (11)
Advanced Verification (1)

FIGURE: 1 / 12

IL-4 Antibody (17-7049-42) in Flow

Frontiers in cell and developmental biology 2022 - FIGURE 2 Cytokine-producing T cells from peripheral blood. PBMCs isolated from moderate-to-severe plaque psoriasis patients and healthy controls were stimulated with Cell Stimulation Cocktail for 5 h. The IFN-gamma-, IL-4- and IL-17-producing T cells were determined by intracellular staining and flow cytometry analysis. (A) The proportion of IFN-gamma-,IL-4- and IL-17-producing CD4 + T cells in psoriasis pa... View More

Product Image Gallery

IL-4 Antibody — Figure 6. piR30840 regulates the development of Th2 lymphocytes. ( A and B ) Human CD4 naive cells were cultured in a conditioned medium for Th2 development and then transfected with an s30840 (A) or piR30840 inhibitor (B). IL-4 expression was detected by qRT-PCR and western blotting analyses. P < 0.01. The data represents three independent experiments. ( C ) Detection of IL-4 expression in human PBMCs isolated from humanized NOG mice. IL-4 expression was detected by qRT-PCR analysis. The data was expressed as the mean of three independent experiments. Wilcoxon test was used to calculate the P -value. P < 0.05. ( D ) CD4 naive cells were cultured in the conditioned medium for Th2 development and transfected with s30840 or control. After 7 days, the Th2 T-lymphocytes (intracellular stained as IL-4 + IFN-gamma - ) were detected with FACS analysis. The data was shown as a mean of six independent experiments. P < 0.01. ( E ) The CD4 naive cells were cultured in a conditioned medium for Th2 development with or without the transfection of inhibitor. We adoptively transferred the naive cells into the irradiated NOG mice which were first humanized. After 7 days, Th2 T-lymphocytes (intracellularly stained as IL-4 + IFNgamma - ) were detected by using FACS analysis. The data were expressed as the mean of six independent experiments. Statistical significance between two samples was determined by using the student's t -test. P < 0.01. {TM}

Product Details

17-7049-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.06 µg)/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

8D4-8

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10852839

Product Specific Information

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported:The 8D4-8 antibody has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining for flow cytometric analysis.

Applications Tested: Has been pre-titrated and tested in intraceµLlµLar staining and flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

17-7049-42 was used in Flow cytometry/Cell sorting to report that human blood CD1c+ dendritic cells activated by GM-CSF (also known as CSF2) drive the differentiation of naive CD4+ T cells into Tfh1 cells.

Tue Nov 01 00:00:00 EDT 2022

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Wed Feb 23 00:00:00 EST 2022

Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively.

International journal of molecular sciences

Perveen K,Quach A,McPhee A,Prescott SL,Barry SC,Hii CS,Ferrante A

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Concomitant suppression of T_H2 and T_H17 cell responses in allergic asthma by targeting retinoic acid receptor-related orphan receptor γt.

The Journal of allergy and clinical immunology

Na H,Lim H,Choi G,Kim BK,Kim SH,Chang YS,Nurieva R,Dong C,Chang SH,Chung Y

17-7049 was used in Flow cytometry/Cell sorting to determine the role of the TH17 cell pathway in regulating TH2 cell responses in allergic asthma.

Fri Jun 01 00:00:00 EDT 2018

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma.

Journal of immunology research

Zhao X,Hua M,Yan S,Yu J,Han F,Zhong C,Wang R,Zhang C,Hou M,Ma D

17-7049 was used in Flow cytometry/Cell sorting to investigate the role of the NLRP3 inflammasome in multiple myeloma.

Fri Nov 09 00:00:00 EST 2018

Effects of Anti-Integrin Treatment With Vedolizumab on Immune Pathways and Cytokines in Inflammatory Bowel Diseases.

Frontiers in immunology

Rath T,Billmeier U,Ferrazzi F,Vieth M,Ekici A,Neurath MF,Atreya R

17-7049 was used in Flow cytometry/Cell sorting to identify genetic, cellular, and immunological mechanisms that define response and failure to vedolizumab treatment.

Mon Sep 09 00:00:00 EDT 2019

The Phenotype of Monocytes in Anterior Uveitis Depends on the HLA-B27 Status.

Frontiers in immunology

Kasper M,Walscheid K,Laffer B,Bauer D,Busch M,Wildschütz L,Wang B,Loser K,Vogl T,Grajewski RS,Langmann T,Heiligenhaus A

Mon Sep 16 00:00:00 EDT 2019

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

Published figure using IL-4 monoclonal antibody (Product # 17-7049-42) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

TIGIT expressing CD4+T cells represent a tumor-supportive T cell subset in chronic lymphocytic leukemia.

Oncoimmunology

Catakovic K,Gassner FJ,Ratswohl C,Zaborsky N,Rebhandl S,Schubert M,Steiner M,Gutjahr JC,Pleyer L,Egle A,Hartmann TN,Greil R,Geisberger R

17-7049 was used in Flow cytometry/Cell sorting to reveal that TIGIT+CD4+T cells provide a supportive microenvironment for CLL cells.

Sat Jan 29 00:00:00 EST 2022

Proteinase 3 on apoptotic cells disrupts immune silencing in autoimmune vasculitis.

The Journal of clinical investigation

Millet A,Martin KR,Bonnefoy F,Saas P,Mocek J,Alkan M,Terrier B,Kerstein A,Tamassia N,Satyanarayanan SK,Ariel A,Ribeil JA,Guillevin L,Cassatella MA,Mueller A,Thieblemont N,Lamprecht P,Mouthon L,Perruche S,Witko-Sarsat V

17-7049 was used in Flow cytometry/Cell sorting to investigate disruption of immune silencing by proteinase 3 on apoptotic cells.

Mon Nov 02 00:00:00 EST 2015

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Nucleic acids research

Zhong F,Zhou N,Wu K,Guo Y,Tan W,Zhang H,Zhang X,Geng G,Pan T,Luo H,Zhang Y,Xu Z,Liu J,Liu B,Gao W,Liu C,Ren L,Li J,Zhou J,Zhang H

Wed Dec 02 00:00:00 EST 2015

DCLRE1C (ARTEMIS) mutations causing phenotypes ranging from atypical severe combined immunodeficiency to mere antibody deficiency.

Human molecular genetics

Volk T,Pannicke U,Reisli I,Bulashevska A,Ritter J,Björkman A,Schäffer AA,Fliegauf M,Sayar EH,Salzer U,Fisch P,Pfeifer D,Di Virgilio M,Cao H,Yang F,Zimmermann K,Keles S,Caliskaner Z,Güner SÜ,Schindler D,Hammarström L,Rizzi M,Hummel M,Pan-Hammarström Q,Schwarz K,Grimbacher B

17-7049 was used in Flow cytometry/Cell sorting to investigate the range of phenotypes associated with DCLRE1C (ARTEMIS) mutations in humans.

Sun Dec 20 00:00:00 EST 2015

CD4+ T cells contain early extrapulmonary tuberculosis (TB) dissemination and rapid TB progression and sustain multieffector functions of CD8+ T and CD3- lymphocytes: mechanisms of CD4+ T cell immunity.

Journal of immunology (Baltimore, Md. : 1950)

Yao S,Huang D,Chen CY,Halliday L,Wang RC,Chen ZW

17-7049 was used in Flow cytometry/Cell sorting to investigate the possibility that CD4(+) T cells can act as innate-like cells to contain very early Mycobacterium tuberculosis dissemination using CD4-depleted macaques.

Sat Mar 01 00:00:00 EST 2014

A polymorphism in TIM1 is associated with susceptibility to severe hepatitis A virus infection in humans.

The Journal of clinical investigation

Kim HY,Eyheramonho MB,Pichavant M,Gonzalez Cambaceres C,Matangkasombut P,Cervio G,Kuperman S,Moreiro R,Konduru K,Manangeeswaran M,Freeman GJ,Kaplan GG,DeKruyff RH,Umetsu DT,Rosenzweig SD

17-7049 was used in Flow cytometry/Cell sorting to investigate the variability in hepatitis disease severity.

Tue Mar 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)

cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

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IL-4 Monoclonal Antibody (8D4-8), APC, eBioscience™

IL-4 Antibody (17-7049-42) in Flow

Product Details

17-7049-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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