Invitrogen

IL-4 Monoclonal Antibody (8D4-8), PE-Cyanine7, eBioscience™

FIGURE: 1 / 12

IL-4 Antibody (25-7049-82) in Flow

Surface staining of normal human peripheral blood cells stimulated with PMA/Ionomycin in the presence of Brefeldin A with Anti-Human CD4 FITC (Product # 11-0048-42), followed by intracellular staining with Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human IL-4 PE-Cyanine7 (right).

Product Image Gallery

Product Details

25-7049-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

8D4-8

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469676

Product Specific Information

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported:The 8D4-8 antibody has been reported for use as a capture antibody for human IL-4 ELISA and for intracellular staining for flow cytometric analysis.

Applications Tested: Has been tested by intracellular flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Limited induction of polyfunctional lung-resident memory T cells against SARS-CoV-2 by mRNA vaccination compared to infection.

Nature communications

Pieren DKJ,Kuguel SG,Rosado J,Robles AG,Rey-Cano J,Mancebo C,Esperalba J,Falcó V,Buzón MJ,Genescà M

25-7049-82 was used in Flow cytometry/Cell sorting to indicate that mRNA vaccination induces specific T cell responses to SARS-CoV-2 in the lung parenchyma, although to a limited extend.

Wed Apr 05 00:00:00 EDT 2023

Regulatory T-cells and IL-5 mediate pain outcomes in a preclinical model of chronic muscle pain.

Molecular pain

Lenert ME,Szabo-Pardi TA,Burton MD

25-7049-82 was used in Flow Cytometry to provide novel evidence that downregulation of regulatory T-cells play a role in chronic muscle pain pathology in the acidic saline model of FM and that IL-5 signaling is a promising target for future development of therapeutics.

Mon Feb 13 00:00:00 EST 2023

A multiclade env-gag VLP mRNA vaccine elicits tier-2 HIV-1-neutralizing antibodies and reduces the risk of heterologous SHIV infection in macaques.

Nature medicine

Zhang P,Narayanan E,Liu Q,Tsybovsky Y,Boswell K,Ding S,Hu Z,Follmann D,Lin Y,Miao H,Schmeisser H,Rogers D,Falcone S,Elbashir SM,Presnyak V,Bahl K,Prabhakaran M,Chen X,Sarfo EK,Ambrozak DR,Gautam R,Martin MA,Swerczek J,Herbert R,Weiss D,Misamore J,Ciaramella G,Himansu S,Stewart-Jones G,McDermott A,Koup RA,Mascola JR,Finzi A,Carfi A,Fauci AS,Lusso P

25-7049-82 was used in Flow Cytometry to conclude that the multiclade env-gag VLP mRNA platform represents a promising approach for the development of an HIV-1 vaccine.

Wed Dec 01 00:00:00 EST 2021

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

25-7049-82 was used in Flow cytometry/Cell sorting to explore the expression of iNKT cells in psoriasis patients and the effect of secukinumab on them.

Wed Feb 23 00:00:00 EST 2022

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

25-7049-82 was used in Flow cytometry/Cell sorting to explore the expression of iNKT cells in psoriasis patients and the effect of secukinumab on them.

Wed Feb 23 00:00:00 EST 2022

Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively.

International journal of molecular sciences

Perveen K,Quach A,McPhee A,Prescott SL,Barry SC,Hii CS,Ferrante A

Published figure using IL-4 monoclonal antibody (Product # 25-7049-82) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using IL-4 monoclonal antibody (Product # 25-7049-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using IL-4 monoclonal antibody (Product # 25-7049-82) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using IL-4 monoclonal antibody (Product # 25-7049-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

Published figure using IL-4 monoclonal antibody (Product # 25-7049-82) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

Published figure using IL-4 monoclonal antibody (Product # 25-7049-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Imbalance between subsets of CD8(+) peripheral blood T cells in patients with chronic obstructive pulmonary disease.

PeerJ

Chen L,Chen G,Zhang MQ,Xiong XZ,Liu HJ,Xin JB,Zhang JC,Wu JH,Meng ZJ,Sun SW

25-7049 was used in Flow cytometry/Cell sorting to analyse systematically, CD8(+) cytotoxic T cell subsets in chronic obstructive pulmonary disease patients.

Mon Aug 22 00:00:00 EDT 2016

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Nucleic acids research

Zhong F,Zhou N,Wu K,Guo Y,Tan W,Zhang H,Zhang X,Geng G,Pan T,Luo H,Zhang Y,Xu Z,Liu J,Liu B,Gao W,Liu C,Ren L,Li J,Zhou J,Zhang H

Published figure using IL-4 monoclonal antibody (Product # 25-7049-82) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2015

Altered expression and editing of miRNA-100 regulates iTreg differentiation.

Nucleic acids research

Negi V,Paul D,Das S,Bajpai P,Singh S,Mukhopadhyay A,Agrawal A,Ghosh B

25-7049 was used in Flow cytometry/Cell sorting to explore the role of miRNA editing in CD4(+) T cell differentiation. CD4(+) T cells are an integral component of the adaptive immune system.

Fri Sep 18 00:00:00 EDT 2015

Regulatory T cells produce profibrotic cytokines in the skin of patients with systemic sclerosis.

The Journal of allergy and clinical immunology

MacDonald KG,Dawson NAJ,Huang Q,Dunne JV,Levings MK,Broady R

25-7049 was used in Flow cytometry/Cell sorting to determine whether Treg cells in the blood and skin of patients with SSc acquired abnormal production of effector cytokines.

Wed Apr 01 00:00:00 EDT 2015

Galectin-9 activates and expands human T-helper 1 cells.

PloS one

Gooden MJ,Wiersma VR,Samplonius DF,Gerssen J,van Ginkel RJ,Nijman HW,Hirashima M,Niki T,Eggleton P,Helfrich W,Bremer E

25-7049 was used in Flow cytometry/Cell sorting to demonstrate that galectin-9 activates resting T-cells and promotes their transition to a T-helper 1 cell phenotype.

Fri Jan 10 00:00:00 EST 2014

Programmed death-1+ T cells and regulatory T cells are enriched in tumor-involved lymph nodes and associated with aggressive features in papillary thyroid cancer.

The Journal of clinical endocrinology and metabolism

French JD,Kotnis GR,Said S,Raeburn CD,McIntyre RC,Klopper JP,Haugen BR

25-7049 was used in Flow cytometry/Cell sorting to characterise the CD4(+) T cell response to metastatic PTC.

Fri Jun 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

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Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

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UniProt ID: (Human) P05112

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Entrez Gene ID: (Human) 3565

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Function(s)

cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-4 Monoclonal Antibody (8D4-8), PE-Cyanine7, eBioscience™

IL-4 Antibody (25-7049-82) in Flow

Product Details

25-7049-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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