Invitrogen

IL-4 Monoclonal Antibody (8D4-8), eFluor™ 450, eBioscience™

FIGURE: 1 / 15

IL-4 Antibody (48-7049-42) in Flow

Normal human peripheral blood cells were stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-93) and then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with CD3 Monoclonal Antibody, APC (Product # 17-0038-42) and Mouse IgG1 kappa Isotype Control, eFluor 450 (Product # 48-4714-82) (left) or IL-4 Monoclonal Antibody, eFluor 450 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

48-7049-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 9 publications 9 publications

ELISA (ELISA)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

8D4-8

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815184

Product Specific Information

Description: The 8D4-8 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.

Applications Reported: This 8D4-8 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 8D4-8 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol A: Two step protocol for intracellular (cytoplasmic) proteins" located at www.thermofisher.com/flowprotocols . This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor 450 is an alternative to Pacific Blue. eFluor 450 emits at 446 nm and is excited with the violet laser line (405 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Target Information

Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Wed Feb 23 00:00:00 EST 2022

Cord Blood T Cells Expressing High and Low PKCζ Levels Develop into Cells with a Propensity to Display Th1 and Th9 Cytokine Profiles, Respectively.

International journal of molecular sciences

Perveen K,Quach A,McPhee A,Prescott SL,Barry SC,Hii CS,Ferrante A

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Platelet-Derived GARP Induces Peripheral Regulatory T Cells-Potential Impact on T Cell Suppression in Patients with Melanoma-Associated Thrombocytosis.

Cancers

Zimmer N,Krebs FK,Zimmer S,Mitzel-Rink H,Kumm EJ,Jurk K,Grabbe S,Loquai C,Tuettenberg A

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Sat Dec 05 00:00:00 EST 2020

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Reciprocal regulation of Th2 and Th17 cells by PAD2-mediated citrullination.

JCI insight

Sun B,Chang HH,Salinger A,Tomita B,Bawadekar M,Holmes CL,Shelef MA,Weerapana E,Thompson PR,Ho IC

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in ELISA

Thu Nov 14 00:00:00 EST 2019

CD4 T cell loss and Th2 and Th17 bias are associated with the severity of severe fever with thrombocytopenia syndrome (SFTS).

Clinical immunology (Orlando, Fla.)

Li MM,Zhang WJ,Weng XF,Li MY,Liu J,Xiong Y,Xiong SE,Zou CC,Wang H,Lu MJ,Yang DL,Peng C,Zheng X

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2018

Impact of Enhancer of Zeste Homolog 2 on T Helper Cell-Mediated Allergic Rhinitis.

Frontiers in immunology

Hou TY,Chen MR,Chou YC,Kan PC,Tsai YT,Cha TL

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

CD16⁺ Monocyte Subset Was Enriched and Functionally Exacerbated in Driving T-Cell Activation and B-Cell Response in Systemic Lupus Erythematosus.

Frontiers in immunology

Zhu H,Hu F,Sun X,Zhang X,Zhu L,Liu X,Li X,Xu L,Shi L,Gan Y,Su Y

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes.

Nucleic acids research

Zhong F,Zhou N,Wu K,Guo Y,Tan W,Zhang H,Zhang X,Geng G,Pan T,Luo H,Zhang Y,Xu Z,Liu J,Liu B,Gao W,Liu C,Ren L,Li J,Zhou J,Zhang H

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in Flow Cytometry

Wed Dec 02 00:00:00 EST 2015

Rapid, multiparameter profiling of cellular secretion using silicon photonic microring resonator arrays.

Journal of the American Chemical Society

Luchansky MS,Bailey RC

Published figure using IL-4 monoclonal antibody (Product # 48-7049-42) in ELISA

Wed Dec 21 00:00:00 EST 2011

Bioinformatics

Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra

View more View less

Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4

View more View less

UniProt ID: (Human) P05112

View more View less

Entrez Gene ID: (Human) 3565

View more View less

Function(s)

cytokine activity interleukin-4 receptor binding protein binding growth factor activity interleukin superfamily

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-4 Monoclonal Antibody (8D4-8), eFluor™ 450, eBioscience™

IL-4 Antibody (48-7049-42) in Flow

Product Details

48-7049-42

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!