Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
IL-4 Monoclonal Antibody (MP4-25D2), Functional Grade, eBioscience™
FIGURE: 1 / 3
IL-4 Antibody (16-7048-81) in Flow
Scientific reports 2023 -
Inhibition of DYRK1B function suppresses Th1 and Th17, but enhances Treg differentiation. ( A-C ) Human naive CD4 T cells were stimulated by anti-CD3 and anti-CD28 and then differentiated under Th1-, Th17- and Th2-polarizing conditions, respectively, in the absence or presence of a selective DYRK1B inhibitor at 4 different concentrations for 96 h. The scatter dot plots represent data from triplicate sample... View More
Product Details
16-7048-81
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rat
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MP4-25D2
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_469210
Product Specific Information
Description: The MP4-25D2 antibody reacts with human interleukin-4 (IL-4), a 15-19 kDa cytokine secreted by Th2 cells.
Applications Reported: MP4-25D2 has been reported for use in neutralizing IL-4 binding and biological activity. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Applications Tested: The MP4-25D2 antibody has been tested for neutralizing IL-4 binding and biological activity.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B cell growth factor 1; B-cell stimulatory factor 1; B_cell stimulatory factor 1; Binetrakin; BSF-1; H-IL-4; IL-4; ILN; Interleukin; interleukin 4 variant 2; Interleukin-4; Interleukin4; Lymphocyte stimulatory factor 1; M-IL-4; MGC79402; Pitrakinra
Gene Aliases: BCGF-1; BCGF1; BSF-1; BSF1; IL-4; IL4
UniProt ID: (Human) P05112
Entrez Gene ID: (Human) 3565
microglial cell activation
innate immune response in mucosa
positive regulation of defense response to virus by host
T-helper 1 cell lineage commitment
negative regulation of acute inflammatory response
negative regulation of chronic inflammatory response
chemotaxis
immune response
cellular defense response
female pregnancy
response to nutrient
cholesterol metabolic process
regulation of proton transport
negative regulation of epithelial cell migration
response to organic cyclic compound
B cell differentiation
positive regulation of B cell proliferation
B cell costimulation
positive regulation of interleukin-10 production
positive regulation of interleukin-13 production
response to cytokine
T-helper 2 cell cytokine production
type 2 immune response
positive regulation of T cell proliferation
positive regulation of activated T cell proliferation
regulation of phosphorylation
response to drug
positive regulation of tyrosine phosphorylation of Stat5 protein
defense response to protozoan
myeloid dendritic cell differentiation
negative regulation of macrophage activation
negative regulation of apoptotic process
positive regulation of mast cell degranulation
negative regulation of nitric oxide biosynthetic process
T-helper 2 cell differentiation
positive regulation of chemokine biosynthetic process
connective tissue growth factor biosynthetic process
regulation of isotype switching
positive regulation of MHC class II biosynthetic process
response to ethanol
positive regulation of T cell differentiation
negative regulation of osteoclast differentiation
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of isotype switching to IgE isotypes
positive regulation of isotype switching to IgG isotypes
regulation of immune response
positive regulation of sequence-specific DNA binding transcription factor activity
retina development in camera-type eye
negative regulation of white fat cell proliferation
cellular response to mercury ion
positive regulation of mononuclear cell migration
dendritic cell differentiation
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of myoblast fusion
negative regulation of complement-dependent cytotoxicity
negative regulation of T-helper 17 cell differentiation
negative regulation of endothelial cell apoptotic process
positive regulation of eosinophil chemotaxis
negative regulation of extrinsic apoptotic signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support