Invitrogen

IL-5 Monoclonal Antibody (TRFK5), Biotin

Product Details

MM550CB

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

View 14 publications 14 publications

Neutralization (Neu)

View 1 publication 1 publication

Blocking Assay (BLOCK)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Hamster, Mouse

Host/Isotype

Rat / IgG1

Class

Monoclonal

Type

Antibody

Clone

TRFK5

Immunogen

Recombinant mouse IL-5

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 4% BSA

Contains

proprietary Preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_223606

Product Specific Information

MM550CB targets IL-5 in ELISA, and WB applications and shows reactivity with mouse samples.

The MM550CB immunogen is recombinant mouse IL-5.

MM550CB detects IL-5 which has a predicted molecular weight of approximately 13 kDa.

The MM550CB mouse IL5 antibody (biotinylated conjugate of clone TRFK5) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody MM550D (clone TRFK4). Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.

Biotinylated antibody MM550CB (clone TRFK5) and antibody MM550D (clone TRFK4) have successfully been used in combination with recombinant mouse IL5 protein SMIL5 in ELISA applications.

This antibody is produced by injecting Rat IgG secreting hybridoma cells into the peritoneum of mice. The resulting ascites is collected from the mice and the antibody is purified.

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Extravascular gelation shrinkage-derived internal stress enables tumor starvation therapy with suppressed metastasis and recurrence.

Nature communications

Zhang K,Fang Y,He Y,Yin H,Guan X,Pu Y,Zhou B,Yue W,Ren W,Du D,Li H,Liu C,Sun L,Chen Y,Xu H

MM550CB was used in an ELISA assay to establish an extravascular gelation shrinkage-derived internal stress strategy for squeezing and narrowing blood vessels, occluding blood & nutrition supply, reducing vascular density, inducing hypoxia and apoptosis and eventually realizing starvation therapy of malignancies.

Tue Nov 26 00:00:00 EST 2019

Inhibition of interleukin-5 induced false positive anti-drug antibody responses against mepolizumab through the use of a competitive blocking antibody.

Journal of immunological methods

Liao K,Meyer E,Lee TN,Loercher A,Sikkema D

MM550CB was used in blocking or activating experiment to compare solid-phase immunodepletion of IL-5 with an in-solution IL-5 immunocompetition

Wed Feb 01 00:00:00 EST 2017

Chronic intestinal nematode infection induces Stat6-independent interleukin-5 production and causes eosinophilic inflammatory responses in mice.

Immunology

Sakamoto Y,Hiromatsu K,Ishiwata K,Inagaki-Ohara K,Ikeda T,Nakamura-Uchiyama F,Nawa Y

MM550CB was used in ELISA to investigate the role of STAT6 for the immune response induced by Nippostrongylus brasiliensis

Sun Aug 01 00:00:00 EDT 2004

Vaccination with recombinant alphavirus or immune-stimulating complex antigen against respiratory syncytial virus.

Journal of immunology (Baltimore, Md. : 1950)

Chen M,Hu KF,Rozell B,Orvell C,Morein B,Liljeström P

MM550CB was used in ELISA to study the vaccination against respiratory syncytial virus

Sun Sep 15 00:00:00 EDT 2002

Effects of birch pollen and traffic particulate matter on Th2 cytokines, immunoglobulin E levels and bronchial hyper-responsiveness in mice.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

Fernvik E,Peltre G,Sénéchal H,Vargaftig BB

MM550CB was used in ELISA to study the role of pollen and traffic particulate matter in the initiation and persistence of experimental allergic inflammation

Mon Apr 01 00:00:00 EST 2002

Murine strain differences in airway inflammation caused by diesel exhaust particles.

The European respiratory journal

Miyabara Y,Yanagisawa R,Shimojo N,Takano H,Lim HB,Ichinose T,Sagai M

MM550CB was used in ELISA to investigate the strain-specific inflammatory responses against air pollutants

Sun Feb 01 00:00:00 EST 1998

Immunization of mice with phosphatidylcholine drastically reduces the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections.

Immunology

Bordmann G,Rudin W,Favre N

MM550CB was used in ELISA to investigate the effect of phosphatidylcholine immunization of mice for the parasitaemia of subsequent Plasmodium chabaudi chabaudi blood-stage infections

Fri May 01 00:00:00 EDT 1998

IL-5-dependent immunity to microfilariae is independent of IL-4 in a mouse model of onchocerciasis.

Journal of immunology (Baltimore, Md. : 1950)

Hogarth PJ,Taylor MJ,Bianco AE

MM550CB was used in ELISA to study the roles of cytokines in helminth immune responses

Mon Jun 01 00:00:00 EDT 1998

Oral administration of L-arginine potentiates allergen-induced airway inflammation and expression of interleukin-5 in mice.

The Journal of pharmacology and experimental therapeutics

Takano H,Lim HB,Miyabara Y,Ichinose T,Yoshikawa T,Sagai M

MM550CB was used in ELISA to investigate the effect of L-arginine administration on airway inflammation and cytokine expression

Sat Aug 01 00:00:00 EDT 1998

Long-term exposure to diesel exhaust enhances antigen-induced eosinophilic inflammation and epithelial damage in the murine airway.

Toxicological sciences : an official journal of the Society of Toxicology

Ichinose T,Takano H,Miyabara Y,Sagai M

MM550CB was used in ELISA to investigate the effect of diesel exhaust on the damage of mouse airway system

Wed Jul 01 00:00:00 EDT 1998

Diesel exhaust particles enhance antigen-induced airway inflammation and local cytokine expression in mice.

American journal of respiratory and critical care medicine

Takano H,Yoshikawa T,Ichinose T,Miyabara Y,Imaoka K,Sagai M

MM550CB was used in ELISA to investigate the effect of diesel exhaust particles on airway inflammation and cytokine expression

Tue Jul 01 00:00:00 EDT 1997

Depletion of eosinophil infiltration by anti-IL-5 monoclonal antibody (TRFK-5) accelerates open skin wound epithelial closure.

The American journal of pathology

Yang J,Torio A,Donoff RB,Gallagher GT,Egan R,Weller PF,Wong DT

MM550CB was used in blocking/activating experiment to study the effect of eosinophil depletion on wound healing

Mon Sep 01 00:00:00 EDT 1997

Allospecific CD8+ Tc1 and Tc2 populations in graft-versus-leukemia effect and graft-versus-host disease.

Journal of immunology (Baltimore, Md. : 1950)

Fowler DH,Breglio J,Nagel G,Eckhaus MA,Gress RE

MM550CB was used in ELISA to study ways to improve allogeneic T cell transfers in leukemia patients

Sun Dec 01 00:00:00 EST 1996

Adjuvant-free hsp70 fusion protein system elicits humoral and cellular immune responses to HIV-1 p24.

Journal of immunology (Baltimore, Md. : 1950)

Suzue K,Young RA

MM550CB was used in ELISA to investigate the immunogenicity of Mycobacterium tuberculosis heat shock proteins

Mon Jan 15 00:00:00 EST 1996

IL-12-induced protection against blood-stage Plasmodium chabaudi AS requires IFN-gamma and TNF-alpha and occurs via a nitric oxide-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Stevenson MM,Tam MF,Wolf SF,Sher A

MM550CB was used in ELISA to study the mechanism for the effect of interleukin 12 on Plasmodium chabaudi

Fri Sep 01 00:00:00 EDT 1995

Synthesis of T helper 2-type cytokines at the maternal-fetal interface.

Journal of immunology (Baltimore, Md. : 1950)

Lin H,Mosmann TR,Guilbert L,Tuntipopipat S,Wegmann TG

MM550CB was used in ELISA to investigate the expression of Th2 cytokines at the maternal-fetal interface

Mon Nov 01 00:00:00 EST 1993

Bioinformatics

Protein Aliases: B-cell growth factor II; BCGF-II; Cytotoxic T-lymphocyte inducer; Eosinophil differentiation factor; IL-5; ILN; Interleukin; Interleukin-5; Interleukin5; T-cell replacing factor; TRF

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Gene Aliases: Il-5; Il5

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UniProt ID: (Mouse) P04401

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Entrez Gene ID: (Mouse) 16191

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Function(s)

cytokine activity interleukin-5 receptor binding growth factor activity

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-5 Monoclonal Antibody (TRFK5), Biotin

Product Details

MM550CB

Western Blot (WB)

ELISA (ELISA)

Neutralization (Neu)

Blocking Assay (BLOCK)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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