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INPP5E Antibody (PA5-103551) in ICC/IF
Immunocytochemistry analysis of INPP5E in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with INPP5E polyclonal antibody (Product # PA5-103551) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-103551
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human INPP5E(Accession Q9NRR6), corresponding to amino acid residues G522-S572.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2852887
Product Specific Information
Antibody detects endogenous levels of total INPP5E.
Target Information
INPP5E (inositol polyphosphate 5-phosphatase), also known as phosphatidylinositol polyphosphate 5-phosphatase type IV, is a 644 amino acid peripheral membrane protein associated with Golgi stacks. Belonging to the inositol-1, 4, 5-trisphosphate 5-phosphatase type IV family, INPP5E converts phosphatidylinositol-3, 4, 5-triphosphate (PtdIns 3, 4, 5-P3) to PtdIns-P2. While inactive towards water soluble inositol phosphates, the activity of INPP5E is specific for lipid substrates. INPP5E becomes phosphorylated upon DNA damage and is expressed in brain, heart, pancreas, testis and spleen. Defects in INPP5E are the cause of Joubert syndrome type 1 (JBTS1), a disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. In addition, mutations in the INPP5E gene may lead to mental retardation-truncal obesity-retinal dystrophy-micropenis (MORMS), an autosomal recessive disorder characterized by moderate mental retardation, truncal obesity, congenital non-progressive retinal dystrophy, and micropenis in males.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5-phosphatase that induces arborization; 72 kDa inositol polyphosphate 5-phosphatase; inositol polyphosphate 5-phosphatase; Inositol polyphosphate-5-phosphatase E; inositol polyphosphate-5-phosphatase, 72 kDa; Pharbin; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; Phosphatidylinositol polyphosphate 5-phosphatase type IV; Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase; phosphatidylinositol-4,5-bisphosphate 5-phosphatase
Gene Aliases: 1200002L24Rik; 72kDa; CORS1; CPD4; INPP5E; JBTS1; mKIAA0123; MORMS; PPI5PIV
UniProt ID: (Human) Q9NRR6, (Rat) Q9WVR1, (Mouse) Q9JII1
Entrez Gene ID: (Human) 56623, (Rat) 114089, (Mouse) 64436
Performance Guarantee
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