Invitrogen

IRF4 Monoclonal Antibody (3E4), eFluor™ 450, eBioscience™

FIGURE: 1 / 16

IRF4 Antibody (48-9858-82) in Flow

Intracellular staining of 3-day Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0031-82 and Product # 16-0281-82)-stimulated human peripheral blood cells with 0.25 µg of Rat IgG1 K Isotype Control eFluor® 450 (Product # 48-4301-82) (blue histogram) or 0.25 µg of Anti-Human/Mouse IRF4 eFluor® 450 (purple histogram). The orange histogram is unstimulated cells stained with 0.25 µg of Anti-Human/Mouse IRF4 eFluor® 450. Viable lymphocytes, as determined by Fixable Viability Dye eFluor® 780, were used for analysis.

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Product Details

48-9858-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 15 publications 15 publications

ELISA (ELISA)

View 1 publication 1 publication

ChIP assay (ChIP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG1, kappa

Recommended Isotype Control

Rat IgG1 kappa Isotype Control (eBRG1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

3E4

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574135

Product Specific Information

Description: The monoclonal antibody 3E4 reacts with human and mouse interferon regulatory factor 4 (IRF4). IRF4 is a 52 kDa transcription factor with roles in B cell, T cell and macrophage function. In B cells, IRF4 is highly expressed in mature plasma cells and plays a crucial role in their differentiation. IRF4 has been shown to interact with PU.1 and control the transcription of many B cell-specific genes including Prdm1, which encodes Blimp1. In T cells, IRF4 has been implicated in regulatory T (Treg), Th2, Th9 and Th17 cell development and function. This transcription factor is upregulated upon T cell activation and is expressed in mature T cells. Studies have shown that IRF4 directly induces Blimp1 expression in Tregs, leading to IL-10 expression. IRF4 has also been demonstrated to be involved in macrophage polarization and regulation. Lastly, in addition to its roles in normal immune function, IRF4 expression has been reported to be upregulated in many blood-related cancers.

Applications Reported:This 3E4 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 3E4 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The IRF (interferon regulatory factor) family of transcription factors are characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. IRF4 is lymphocyte specific and negatively regulates toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Toll-like receptor mediated inflammation directs B cells towards protective antiviral extrafollicular responses.

Nature communications

Lam JH,Baumgarth N

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Wed Jul 05 00:00:00 EDT 2023

Translation factor eIF5a is essential for IFNγ production and cell cycle regulation in primary CD8⁺ T lymphocytes.

Nature communications

Tan TCJ,Kelly V,Zou X,Wright D,Ly T,Zamoyska R

48-9858-82 was used in Flow cytometry/Cell sorting to show that the function of the elongation factor, eIF5a, is regulated dynamically in naive CD8+ T cells upon activation by post-translational modification, whereupon it facilitates translation of specific subsets of proteins.

Sat Dec 17 00:00:00 EST 2022

B cells promote CD8 T cell primary and memory responses to subunit vaccines.

Cell reports

Klarquist J,Cross EW,Thompson SB,Willett B,Aldridge DL,Caffrey-Carr AK,Xu Z,Hunter CA,Getahun A,Kedl RM

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Tue Aug 24 00:00:00 EDT 2021

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Tue Feb 11 00:00:00 EST 2020

Pro-Survival Lipid Sphingosine-1-Phosphate Metabolically Programs T Cells to Limit Anti-tumor Activity.

Cell reports

Chakraborty P,Vaena SG,Thyagarajan K,Chatterjee S,Al-Khami A,Selvam SP,Nguyen H,Kang I,Wyatt MW,Baliga U,Hedley Z,Ngang RN,Guo B,Beeson GC,Husain S,Paulos CM,Beeson CC,Zilliox MJ,Hill EG,Mehrotra M,Yu XZ,Ogretmen B,Mehrotra S

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in ELISA

Tue Aug 13 00:00:00 EDT 2019

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

Blimp-1 Functions as a Molecular Switch to Prevent Inflammatory Activity in Foxp3⁺RORγt⁺ Regulatory T Cells.

Cell reports

Ogawa C,Bankoti R,Nguyen T,Hassanzadeh-Kiabi N,Nadeau S,Porritt RA,Couse M,Fan X,Dhall D,Eberl G,Ohnmacht C,Martins GA

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in ChIP assay

Tue Oct 02 00:00:00 EDT 2018

ROCK2, but not ROCK1 interacts with phosphorylated STAT3 and co-occupies TH17/TFH gene promoters in TH17-activated human T cells.

Scientific reports

Chen W,Nyuydzefe MS,Weiss JM,Zhang J,Waksal SD,Zanin-Zhorov A

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Fri Nov 09 00:00:00 EST 2018

2D Kinetic Analysis of TCR and CD8 Coreceptor for LCMV GP33 Epitopes.

Frontiers in immunology

Kolawole EM,Andargachew R,Liu B,Jacobs JR,Evavold BD

48-9858 was used in Flow cytometry/Cell sorting to study TCR interaction in closely related GP33 variants.

Mon Sep 30 00:00:00 EDT 2019

Batf3 selectively determines acquisition of CD8⁺ dendritic cell phenotype and function.

Immunology and cell biology

Chandra J,Kuo PT,Hahn AM,Belz GT,Frazer IH

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

Ubiquitin specific peptidase 4 stabilizes interferon regulatory factor protein and promotes its function to facilitate interleukin-4 expression in T helper type 2 cells.

International journal of molecular medicine

Guo Z,Xu P,Ge S,Zhang C,Zheng X,Xu J,Liu Z,Li B,Ge S

Sun Oct 01 00:00:00 EDT 2017

γ-Tocopherol supplementation of allergic female mice augments development of CD11c+CD11b+ dendritic cells in utero and allergic inflammation in neonates.

American journal of physiology. Lung cellular and molecular physiology

Abdala-Valencia H,Soveg F,Cook-Mills JM

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4.

Nature communications

Gomez-Rodriguez J,Meylan F,Handon R,Hayes ET,Anderson SM,Kirby MR,Siegel RM,Schwartzberg PL

48-9858 was used in Flow cytometry/Cell sorting to investigate the role of IL-9 in allergic asthma and autoimmunity, showing that Itk is required for Th9 differentiation via TCR-mediated induction of IL-2 and IRF4.

Thu Mar 03 00:00:00 EST 2016

Cell-intrinsic expression of TLR9 in autoreactive B cells constrains BCR/TLR7-dependent responses.

Journal of immunology (Baltimore, Md. : 1950)

Nündel K,Green NM,Shaffer AL,Moody KL,Busto P,Eilat D,Miyake K,Oropallo MA,Cancro MP,Marshak-Rothstein A

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Sun Mar 15 00:00:00 EDT 2015

Beta-catenin signaling drives differentiation and proinflammatory function of IRF8-dependent dendritic cells.

Journal of immunology (Baltimore, Md. : 1950)

Cohen SB,Smith NL,McDougal C,Pepper M,Shah S,Yap GS,Acha-Orbea H,Jiang A,Clausen BE,Rudd BD,Denkers EY

Published figure using IRF4 monoclonal antibody (Product # 48-9858-82) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2015

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals.

Nature immunology

Salmond RJ,Brownlie RJ,Morrison VL,Zamoyska R

48-9858 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity.

Mon Sep 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; NF-EM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP

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Gene Aliases: AI385587; IRF-4; IRF4; LSIRF; MUM1; NF-EM5; SHEP8; Spip

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UniProt ID: (Human) Q15306, (Mouse) Q64287

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Entrez Gene ID: (Human) 3662, (Mouse) 16364

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Function(s)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein-lysine N-methyltransferase activity sequence-specific DNA binding regulatory region DNA binding DNA binding winged helix/forkhead transcription factor

Process(es)

transcription from RNA polymerase II promoter peptidyl-lysine methylation negative regulation of toll-like receptor signaling pathway T cell activation defense response to protozoan myeloid dendritic cell differentiation positive regulation of DNA binding histone H3 acetylation histone H4 acetylation positive regulation of interleukin-10 biosynthetic process positive regulation of interleukin-2 biosynthetic process positive regulation of interleukin-13 biosynthetic process positive regulation of interleukin-4 biosynthetic process regulation of T-helper cell differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter interferon-gamma-mediated signaling pathway type I interferon signaling pathway T-helper 17 cell lineage commitment transcription, DNA-templated regulation of transcription, DNA-templated protein methylation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IRF4 Monoclonal Antibody (3E4), eFluor™ 450, eBioscience™

IRF4 Antibody (48-9858-82) in Flow

Product Details

48-9858-82

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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