Invitrogen

IgM Monoclonal Antibody (SA-DA4), eBioscience™

FIGURE: 1 / 3

IgM Antibody (14-9998-82) in Flow

Staining of prelysed normal human peripheral blood cells with Anti-Human IgM Purified followed by Anti-Mouse IgG FITC.Appropriate isotype controls were used (open histogram).Cells in the lymphocyte population were used for analysis.

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Product Details

14-9998-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

Assay-Dependent

Flow Cytometry (Flow)

1 µg/test

View 3 publications 3 publications

ELISA (ELISA)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

SA-DA4

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468683

Product Specific Information

Description: The SA-DA4 monoclonal antibody reacts with the mu heavy chain of human IgM. It does not react with other classes of human immunoglobulin including IgD, IgG, or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of mature B cells.

Our testing has revealed variations in staining of the non-B cell population that is predicted to be non-specific. This variation can be attributed to both donor variability and the anticoagulant used during blood draw. Therefore, we recommend using sodium citrate as the anticoagulant to minimize non-specific staining by this antibody.

Applications Reported: The SA-DA4 antibody has been reported for use in flow cytometric analysis, immunohistochemical staining of frozen tissue sections, and ELISA. It has also been reported for use in affinity purification of human IgM.

Applications Tested: The SA-DA4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Synthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells.

Frontiers in immunology

Friedensohn S,Lindner JM,Cornacchione V,Iazeolla M,Miho E,Zingg A,Meng S,Traggiai E,Reddy ST

Published figure using IgM monoclonal antibody (Product # 14-9998-82) in Flow Cytometry

Wed Nov 20 00:00:00 EST 2019

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using IgM monoclonal antibody (Product # 14-9998-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

CD20+ B cell depletion alters T cell homing.

Journal of immunology (Baltimore, Md. : 1950)

Kap YS,van Driel N,Laman JD,Tak PP,'t Hart BA

14-9998 was used in Flow cytometry/Cell sorting to investigate the underlying mechanisms of CD20 depletion in the treatment of autoimmune inflammatory disorders, showing that CD20+ B cell depletion alters T cell homing.

Thu May 01 00:00:00 EDT 2014

B cell development in GALT: role of bacterial superantigen-like molecules.

Journal of immunology (Baltimore, Md. : 1950)

Severson KM,Mallozzi M,Driks A,Knight KL

14-9998 was used in Immunofluorescence to provide direct evidence that B cell development in gut-associated lymphoid tissue (GLAT) may be driven by superantigen-like molecules, and that bacterial spores modulate host immunity.

Tue Jun 15 00:00:00 EDT 2010

Bioinformatics

Gene Aliases: AGM1; MU; VH

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Entrez Gene ID: (Human) 3507