Invitrogen

Lymphotactin Polyclonal Antibody, Biotin, PeproTech®

View all (15) Lymphotactin antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

500-P40BT-25UG

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.2 µg/mL
-

ELISA (ELISA)

0.25-1.0 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived Recombinant Human Lymphotactin (XCL1)
View immunogen

Conjugate

Biotin Biotin Biotin

Form

Lyophilized

Concentration

0.1-1.0 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

RRID

AB_2930519

Product Specific Information

AA Sequence of recombinant protein: GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Lymphotactin (XCL1). Anti-Human Lymphotactin (XCL1)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hLymphotactin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Lymphotactin (500-P40) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hLymphotactin.

Western Blot: To detect hLymphotactin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hLymphotactin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P40BT-1MG will be provided as 2 x 500 µg

Target Information

Lymphotactin, otherwise known as XCL1, is a member of the C-chemokine family that uniquely acts as a chemoattractant for lymphocytes, but not monocytes or neutrophils, suggesting an important regulatory role in lymphocyte trafficking and inflammation. Lymphotactin, shown to signal through chemokine XC receptor 1 (XCR1), is detected at the highest level in the spleen and at low levels in the lungs, small intestine and colon, and is abundantly and selectively expressed by activated T cells, in particular CD8+ T cells, and is also produced by NK cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATAC; C motif chemokine 1; chemokine (C motif) ligand 1; Cytokine SCM-1; Lymphotactin; Lymphotaxin; SCM-1-alpha; SCM-1-beta; SCM-1alpha; SCM-1beta; SCM1alpha; SCM1beta; single cysteine motif 1a; small inducible cytokine subfamily C, member 1 (lymphotactin); Small-inducible cytokine C1; XC chemokine ligand 1

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Gene Aliases: ATAC; LPTN; LTN; SCM-1; SCM-1a; SCM1; SCM1A; SCYC1; XCL1

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UniProt ID: (Human) P47992

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Entrez Gene ID: (Human) 6375

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Function(s)
chemokine activity chemokine receptor binding protein homodimerization activity CCR chemokine receptor binding cytokine intercellular signal molecule
Process(es)
positive regulation of T cell mediated cytotoxicity monocyte chemotaxis positive regulation of leukocyte chemotaxis negative regulation of T cell cytokine production positive regulation of T cell cytokine production negative regulation of T-helper 1 type immune response signal transduction G-protein coupled receptor signaling pathway cell-cell signaling response to virus positive regulation of T cell chemotaxis neutrophil chemotaxis negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-10 production mature natural killer cell chemotaxis negative regulation of sequence-specific DNA binding transcription factor activity positive regulation of GTPase activity negative regulation of transcription, DNA-templated regulation of inflammatory response positive regulation of inflammatory response release of sequestered calcium ion into cytosol positive regulation of release of sequestered calcium ion into cytosol chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade cellular response to interferon-gamma cellular response to interleukin-1 cellular response to interleukin-4 cellular response to tumor necrosis factor cellular response to transforming growth factor beta stimulus positive regulation of transforming growth factor beta production positive regulation of granzyme B production positive regulation of neutrophil chemotaxis positive regulation of thymocyte migration positive regulation of natural killer cell chemotaxis positive regulation of granzyme A production negative regulation of T-helper 1 cell activation positive regulation of B cell chemotaxis positive regulation of T-helper 2 cell cytokine production positive regulation of T-helper 1 cell cytokine production positive regulation of immunoglobulin production in mucosal tissue negative regulation of CD4-positive, alpha-beta T cell proliferation positive regulation of CD4-positive, alpha-beta T cell proliferation positive regulation of CD8-positive, alpha-beta T cell proliferation
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