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Invitrogen
OCT4 Monoclonal Antibody (9B7), DyLight™ 488
Advanced Verification
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 4
OCT4 Antibody (MA1-104-D488) in ICC/IF
Immunofluorescence analysis of OCT-4 was performed using 70% confluent log phase NTERA-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with OCT-4 Mouse Monoclonal Antibody (Product # MA1-104-D488) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-ac... View More
Product Details
MA1-104-D488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
9B7
Immunogen
Full length human recombinant protein of human POU5F1 produced in E.coli.
Conjugate
Excitation/Emission Max
492/519 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with proprietary stabilizer
Contains
0.02% sodium azide
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536809
Product Specific Information
MA1-104-D488 detects Oct4/Pou5F1 in human iPS samples.
MA1-104-D488 has been successfully used in ICC/IF applications.
Target Information
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2
Gene Aliases: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU5F1
UniProt ID: (Human) Q01860
Entrez Gene ID: (Human) 5460
transcription regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ubiquitin protein ligase binding
miRNA binding
sequence-specific DNA binding
poly(A) RNA binding
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
blastocyst development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to wounding
anatomical structure morphogenesis
regulation of asymmetric cell division
regulation of gene expression
somatic stem cell population maintenance
mRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
negative regulation of gene silencing by miRNA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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