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OCT4 Antibody (MA1-104-HRP) in WB
Western blot analysis of Oct4/Pou5F1 was performed by loading 50 µg of NCCIT and negative control HeLa cell lysates per well onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane. The membrane was blocked, and probed with an HRP-conjugated Oct4/Pou5F1 monoclonal antibody (Product # MA1-104-HRP) at a dilution of 1:1000 overnight at 4C. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
Product Details
MA1-104-HRP
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
9B7
Immunogen
Full length human recombinant protein of human POU5F1 produced in E.coli.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
proprietary buffer with proprietary stabilizer
Contains
0.07% Kathon
Storage conditions
4° C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2536810
Product Specific Information
MA1-104-HRP detects Oct4/Pou5F1 in human embryonic carcinoma cells.
By Western blot, this antibody detects a band at 40 kDa in human embryonal carcinoma cells, but not in negative control cells.
Target Information
POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2
Gene Aliases: Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; POU5F1
UniProt ID: (Human) Q01860
Entrez Gene ID: (Human) 5460
transcription regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
ubiquitin protein ligase binding
miRNA binding
sequence-specific DNA binding
poly(A) RNA binding
negative regulation of transcription from RNA polymerase II promoter
endodermal cell fate specification
blastocyst development
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
response to wounding
anatomical structure morphogenesis
regulation of asymmetric cell division
regulation of gene expression
somatic stem cell population maintenance
mRNA transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter
negative regulation of gene silencing by miRNA
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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