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CD44 Monoclonal Antibody (IM7.8.1), FITC

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CD44 Antibody (MA5-17878) in Flow

Flow cytometric analysis of Spleen cells stained using anti-CD44/H-CAM Monoclonal Antibody (Product # MA5-17878) at a 0.5 µg/10^6 cells dilution. The Isotype control was a FITC Rat IgG2b

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Product Details

MA5-17878

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

IM7.8.1

Immunogen

Mouse CD44

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539262

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Complement-activating IgM enhances the humoral but not the T cell immune response in mice.

PloS one

Ding Z,Bergman A,Rutemark C,Ouchida R,Ohno H,Wang JY,Heyman B

MA5-17878 was used in flow cytometry to confirm the importance of complement for the ability of specific IgM to enhance antibody responses.

Mon Mar 30 00:00:00 EDT 2015

Developmental dynamics of post-selection thymic DN iNKT.

PloS one

Yassai M,Cooley B,Gorski J

MA5-17878 was used in flow cytometry to study the kinetics of iNKT cell precursors

Tue Jan 29 00:00:00 EST 2013

Development of memory-like autoregulatory CD8+ T cells is CD4+ T cell dependent.

Journal of immunology (Baltimore, Md. : 1950)

Shameli A,Clemente-Casares X,Wang J,Santamaria P

MA5-17878 was used in flow cytometry to report that development of autoregulatory CD8(+) T cell memory is CD4(+) T cell dependent.

Thu Sep 15 00:00:00 EDT 2011

Yersinia enterocolitica promotes robust mucosal inflammatory T-cell immunity in murine neonates.

Infection and immunity

Echeverry A,Saijo S,Schesser K,Adkins B

MA5-17878 was used in flow cytometry to investigate the mucosal inflammatory T-cell immunity induced by Yersinia enterocolitica infection

Sun Aug 01 00:00:00 EDT 2010

Studies on the mechanism by which antigen-specific IgG suppresses primary antibody responses: evidence for epitope masking and decreased localization of antigen in the spleen.

Scandinavian journal of immunology

Getahun A,Heyman B

MA5-17878 was used in flow cytometry to study mechanisms of IgG-mediated suppression

Tue Sep 01 00:00:00 EDT 2009

Self-organized criticality theory of autoimmunity.

PloS one

Tsumiyama K,Miyazaki Y,Shiozawa S

MA5-17878 was used in flow cytometry to discuss causes of autoimmunity

Thu Dec 31 00:00:00 EST 2009

Defective CD4+CD25+ regulatory T cell functioning in collagen-induced arthritis: an important factor in pathogenesis, counter-regulated by endogenous IFN-gamma.

Arthritis research & therapy

Kelchtermans H,De Klerck B,Mitera T,Van Balen M,Bullens D,Billiau A,Leclercq G,Matthys P

MA5-17878 was used in flow cytometry to show that the decrease in T regulatory cell activity in collagen-induced arthritis is counter-regulated by endogenous IFN-gamma

Thu Dec 15 00:00:00 EST 2005

IgE enhances antibody and T cell responses in vivo via CD23+ B cells.

Journal of immunology (Baltimore, Md. : 1950)

Getahun A,Hjelm F,Heyman B

MA5-17878 was used in flow cytometry to test if IgE antibodies modulate T helper cells in vivo

Mon Aug 01 00:00:00 EDT 2005

Involvement of p38 mitogen-activated protein kinase in different stages of thymocyte development.

Blood

Hsu SC,Wu CC,Han J,Lai MZ

MA5-17878 was used in flow cytometry to suggest that p38 is involved in positive selection of T cells.

Sat Feb 01 00:00:00 EST 2003

Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection.

Molecular and cellular biology

Yu CT,Feng MH,Shih HM,Lai MZ

MA5-17878 was used in flow cytometry to test if T-cell-specific expression of p300 alters GR-TCR cross talk between thymocytes.

Mon Jul 01 00:00:00 EDT 2002

Immature multipotent hemopoietic progenitors lacking long-term bone marrow-reconstituting activity in the aorta-gonad-mesonephros region of murine day 10 fetuses.

Journal of immunology (Baltimore, Md. : 1950)

Ohmura K,Kawamoto H,Lu M,Ikawa T,Ozaki S,Nakao K,Katsura Y

MA5-17878 was used in flow cytometry to examine multipotent progenitors from early fetuses.

Thu Mar 01 00:00:00 EST 2001

Costimulation light: activation of CD4+ T cells with CD80 or CD86 rather than anti-CD28 leads to a Th2 cytokine profile.

Journal of immunology (Baltimore, Md. : 1950)

Broeren CP,Gray GS,Carreno BM,June CH

MA5-17878 was used in flow cytometry to determine the role of CD28 and CTLA-4 in Th cell differentiation.

Fri Dec 15 00:00:00 EST 2000

Defective development of gamma/delta T cells in interleukin 7 receptor-deficient mice is due to impaired expression of T cell receptor gamma genes.

The Journal of experimental medicine

Kang J,Coles M,Raulet DH

MA5-17878 was used in flow cytometry to determine the role of IL-7R signaling in gamma/delta T cell development.

Mon Oct 04 00:00:00 EDT 1999

Class I MHC molecules on hematopoietic cells can support intrathymic positive selection of T cell receptor transgenic T cells.

Proceedings of the National Academy of Sciences of the United States of America

Zerrahn J,Volkmann A,Coles MC,Held W,Lemonnier FA,Raulet DH

MA5-17878 was used in flow cytometry to identify cells that mediate positive selection of CD8(+) T cells.

Tue Sep 28 00:00:00 EDT 1999

Memory CD44(int) CD8 T cells show increased proliferative responses and IFN-gamma production following antigenic challenge in vitro.

International immunology

Pihlgren M,Arpin C,Walzer T,Tomkowiak M,Thomas A,Marvel J,Dubois PM

MA5-17878 was used in flow cytometry to investigate hyper responsive CD8 T cells.

Sat May 01 00:00:00 EDT 1999

Differential chemotactic behavior of developing T cells in response to thymic chemokines.

Blood

Kim CH,Pelus LM,White JR,Broxmeyer HE

MA5-17878 was used in flow cytometry to determine the chemotactic responsiveness of thymocyte subsets during their early to late stages of development.

Mon Jun 15 00:00:00 EDT 1998

Resting memory CD8+ T cells are hyperreactive to antigenic challenge in vitro.

The Journal of experimental medicine

Pihlgren M,Dubois PM,Tomkowiak M,Sjögren T,Marvel J

MA5-17878 was used in flow cytometry to test if different activation thresholds distinguish naive from memory CD8+ T cell populations.

Sun Dec 01 00:00:00 EST 1996

Bioinformatics

Protein Aliases: CD44; CD44 antigen; CD44 molecule; ECMR-III; ECMRIII; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44

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Gene Aliases: AU023126; AW121933; AW146109; Cd44; HERMES; Ly-24; Pgp-1

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UniProt ID: (Mouse) P15379

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Entrez Gene ID: (Mouse) 12505

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Function(s)

hyalurononglucosaminidase activity cytokine receptor activity epidermal growth factor receptor binding protein binding hyaluronic acid binding protein kinase binding cytokine binding phosphoprotein binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD44 Monoclonal Antibody (IM7.8.1), FITC

CD44 Antibody (MA5-17878) in Flow

Product Details

MA5-17878

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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