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CD44 Monoclonal Antibody (IM7.8.1), PE

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CD44 Antibody (MA5-17880) in Flow

Flow cytometric analysis of Spleen Cells staining using anti-CD44/H-CAM Monoclonal Antibody (Product # MA5-17880) at a 0.5 µg/10^6 cells dilution. The Isotypic control was a PE Rat IgG2b

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Product Details

MA5-17880

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/1x10^6 cells

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

IM7.8.1

Immunogen

Mouse CD44

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 4-5mg/mL BSA

Contains

0.02% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2539264

Target Information

CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Ultraviolet B suppresses immunity by inhibiting effector and memory T cells.

The American journal of pathology

Rana S,Byrne SN,MacDonald LJ,Chan CY,Halliday GM

MA5-17880 was used in flow cytometry to elucidate whether UVB exposure inhibits the expansion of effector CD4+ and CD8+ T cells in skin-draining lymph nodes and reduces the number of CD4+ and IFN-gamma+ CD8+ T cells at sites of challenge

Tue Apr 01 00:00:00 EDT 2008

Accumulation of effector CD4 T cells during type 2 immune responses is negatively regulated by Stat6.

Journal of immunology (Baltimore, Md. : 1950)

King SB,Knorn AM,Ohnmacht C,Voehringer D

MA5-17880 was used in flow cytometry to investigate the effect of Stat6 in T cell expansion, survival, and recruitment to the lung

Tue Jan 15 00:00:00 EST 2008

An intense form of homeostatic proliferation of naive CD8+ cells driven by IL-2.

The Journal of experimental medicine

Cho JH,Boyman O,Kim HO,Hahm B,Rubinstein MP,Ramsey C,Kim DM,Surh CD,Sprent J

MA5-17881 was used in Flow Cytometry to describe a novel form of homeostatic proliferation that occurs when naive T cells encounter raised levels of IL-2 and IL-15 in vivo.

Mon Aug 06 00:00:00 EDT 2007

Involvement of p38 mitogen-activated protein kinase in different stages of thymocyte development.

Blood

Hsu SC,Wu CC,Han J,Lai MZ

MA5-17880 was used in flow cytometry to suggest that p38 is involved in positive selection of T cells.

Sat Feb 01 00:00:00 EST 2003

Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection.

Molecular and cellular biology

Yu CT,Feng MH,Shih HM,Lai MZ

MA5-17880 was used in flow cytometry to test if T-cell-specific expression of p300 alters GR-TCR cross talk between thymocytes.

Mon Jul 01 00:00:00 EDT 2002

Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay.

The Journal of experimental medicine

Ikawa T,Kawamoto H,Fujimoto S,Katsura Y

MA5-17880 was used in flow cytometry to describe a clonal system that supports the development of T and natural killer cells evenly.

Mon Dec 06 00:00:00 EST 1999

Induction of Th2 cell differentiation in the primary immune response: dendritic cells isolated from adherent cell culture treated with IL-10 prime naive CD4+ T cells to secrete IL-4.

International immunology

Liu L,Rich BE,Inobe J,Chen W,Weiner HL

MA5-17880 was used in flow cytometry to identify the initial source of IL-4 in early immune responses.

Sat Aug 01 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: CD44; CD44 antigen; CD44 molecule; ECMR-III; ECMRIII; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; Hermes antigen; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44

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Gene Aliases: AU023126; AW121933; AW146109; Cd44; HERMES; Ly-24; Pgp-1

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UniProt ID: (Mouse) P15379

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Entrez Gene ID: (Mouse) 12505

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Function(s)

hyalurononglucosaminidase activity cytokine receptor activity epidermal growth factor receptor binding protein binding hyaluronic acid binding protein kinase binding cytokine binding phosphoprotein binding transmembrane signal receptor

Process(es)

Performance Guarantee

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CD44 Monoclonal Antibody (IM7.8.1), PE

CD44 Antibody (MA5-17880) in Flow

Product Details

MA5-17880

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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