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CD8 alpha Monoclonal Antibody (5H10), PE

CD8 alpha Antibody in Flow Cytometry (Flow)

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CD8 alpha Antibody (MCD0804) in Flow

Tuberculosis (Edinburgh, Scotland) 2011 - Published figure using CD8 alpha monoclonal antibody (Product # MCD0804) in Flow Cytometry

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Product Details

MCD0804

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 13 publications 13 publications

ELISA (ELISA)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5H10

Immunogen

Con A-stimulated BALB/c splenic T cells

View immunogen

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373415

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A candidate subunit vaccine induces protective immunity against Mycobacterium avium subspecies paratuberculosis in mice.

NPJ vaccines

Shao M,Cui N,Tang Y,Chen F,Cui Y,Dang G,Liu S

MCD0804 was used in ELISA to demonstrate that recombinant protein 66NC is an efficient candidate for further development into a protective vaccine in terms of inducing specific protection against MAP.

Sat May 20 00:00:00 EDT 2023

M2 polarization of tumor-associated macrophages is dependent on integrin β3 via peroxisome proliferator-activated receptor-γ up-regulation in breast cancer.

Immunology

Shu Y,Qin M,Song Y,Tang Q,Huang Y,Shen P,Lu Y

MCD0804 was used in Flow Cytometry to find a connection between integrin β3 and macrophage polarization.

Sat Aug 01 00:00:00 EDT 2020

Single-cell analysis reveals sexually dimorphic repertoires of Interferon-γ and IL-17A producing T cells in salivary glands of Sjögren's syndrome mice.

Scientific reports

Wanchoo A,Voigt A,Sukumaran S,Stewart CM,Bhattacharya I,Nguyen CQ

MCD0804 was used in Flow cytometry to investigate the clonal diversity of T cell receptors in interferon-γ and IL-17A producing T cells in salivary glands of Sjogren's syndrome mice.

Mon Oct 02 00:00:00 EDT 2017

CD4+ Foxp3+ regulatory T cells mediate Toxoplasma gondii-induced T-cell suppression through an IL-2-related mechanism but independently of IL-10.

European journal of immunology

Tenorio EP,Fernández J,Castellanos C,Olguín JE,Saavedra R

MCD0804 was used in Flow cytometry/Cell sorting to study the role of Treg cells in Toxoplasma gondii induced suppression.

Thu Dec 01 00:00:00 EST 2011

The ΔfbpA attenuated candidate vaccine from Mycobacterium tuberculosis, H37Rv primes for a stronger T-bet dependent Th1 immunity in mice.

Tuberculosis (Edinburgh, Scotland)

Roche CM,Smith A,Lindsey DR,Meher A,Schluns K,Arora A,Armitige LY,Jagannath C

Published figure using CD8 alpha monoclonal antibody (Product # MCD0804) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2011

B7-H1-dependent sex-related differences in tumor immunity and immunotherapy responses.

Journal of immunology (Baltimore, Md. : 1950)

Lin PY,Sun L,Thibodeaux SR,Ludwig SM,Vadlamudi RK,Hurez VJ,Bahar R,Kious MJ,Livi CB,Wall SR,Chen L,Zhang B,Shin T,Curiel TJ

MCD0804 was used in flow cytometry to report sex-dependent, B7-H1-dependent differences in tumor immunity

Wed Sep 01 00:00:00 EDT 2010

Lumican is required for neutrophil extravasation following corneal injury and wound healing.

Journal of cell science

Hayashi Y,Call MK,Chikama T,Liu H,Carlson EC,Sun Y,Pearlman E,Funderburgh JL,Babcock G,Liu CY,Ohashi Y,Kao WW

Published figure using CD8 alpha monoclonal antibody (Product # MCD0804) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2010

CD4+ T cells are required for the priming of CD8+ T cells following infection with herpes simplex virus type 1.

Journal of virology

Rajasagi NK,Kassim SH,Kollias CM,Zhao X,Chervenak R,Jennings SR

MCD0804 was used in flow cytometry to determine the contribution of CD4+ T cells in the generation of CD8+-T-cell responses following acute infection with HSV-1

Fri May 01 00:00:00 EDT 2009

A single naive CD8+ T cell precursor can develop into diverse effector and memory subsets.

Immunity

Stemberger C,Huster KM,Koffler M,Anderl F,Schiemann M,Wagner H,Busch DH

MCD0804 was used in Flow cytometry/Cell sorting to study how the transfer of a single antigen-specific naive T cell into a normal recipient mouse allows recovery of clonally expanded daughter cells upon immunisation.

Sat Dec 01 00:00:00 EST 2007

Myeloproliferative defects following targeting of the Drf1 gene encoding the mammalian diaphanous related formin mDia1.

Cancer research

Peng J,Kitchen SM,West RA,Sigler R,Eisenmann KM,Alberts AS

MCD0804 was used in flow cytometry to indicate that defective DRF1 expression or mammalian diaphanous 1 function contributes to myeloid malignancies and suggest mammalian diaphanous 1 as an attractive therapeutic target in human myeloproliferative syndro

Wed Aug 15 00:00:00 EDT 2007

Characterization of early gamma interferon (IFN-gamma) expression during murine listeriosis: identification of NK1.1+ CD11c+ cells as the primary IFN-gamma-expressing cells.

Infection and immunity

Chang SR,Wang KJ,Lu YF,Yang LJ,Chen WJ,Lin YH,Chang HH,Wang SL

MCD0804 was used in flow cytometry to identify the cells that are producing gamma interferon during infection with Listeria monocytogenes

Thu Mar 01 00:00:00 EST 2007

Efficient induction of tumor antigen-specific CD8+ memory T cells by recombinant lentivectors.

Cancer research

Chapatte L,Colombetti S,Cerottini JC,Lévy F

MCD0804 was used in flow cytometry to study the use of a recombinant lentivector to immunize against the melanoma-associated antigen

Sun Jan 15 00:00:00 EST 2006

Targeted delivery of the ErbB2/HER2 tumor antigen to professional APCs results in effective antitumor immunity.

Journal of immunology (Baltimore, Md. : 1950)

Rohrbach F,Weth R,Kursar M,Sloots A,Mittrücker HW,Wels WS

MCD0804 was used in flow cytometry to optimize the targeted delivery of the ErbB2 tumor antigen to antigen presenting cells in vivo

Sun May 01 00:00:00 EDT 2005

A transgenic mouse model for T-cell ignorance of a glial autoantigen.

Journal of autoimmunity

Cabarrocas J,Piaggio E,Zappulla JP,Desbois S,Mars LT,Lassmann H,Liblau RS

MCD0804 was used in flow cytometry to determine the fate of autoreactive CD4+T cells in HNT-TCR x GFAP-HA double transgenic mice

Sat May 01 00:00:00 EDT 2004

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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Function(s)

protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 alpha Monoclonal Antibody (5H10), PE

CD8 alpha Antibody (MCD0804) in Flow

Product Details

MCD0804

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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