Invitrogen

CD8 alpha Monoclonal Antibody (5H10), PE-Texas Red

CD8 alpha Antibody in Flow Cytometry (Flow)

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CD8 alpha Antibody (MCD0817) in Flow

Journal of immunology (Baltimore, Md. : 1950) 2009 - Published figure using CD8 alpha monoclonal antibody (Product # MCD0817) in Flow Cytometry

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Product Details

MCD0817

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-dependent

View 13 publications 13 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

5H10

Immunogen

Con A-stimulated BALB/c splenic T cells

View immunogen

Conjugate

PE-Texas Red PE-Texas Red PE-Texas Red

Excitation/Emission Max

567/615 nm View spectra

Form

Liquid

Purification

Affinity chromatography

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10374589

Product Specific Information

The 5H10 monoclonal antibody (mAb) recognizes the mouse CD8a chain (Ly-2). Antibodies specific for CD8a rather than CD8b are recommended for a rigorous delineation of CD8 positive T cells. The 5H10 mAb has been used in other labs for flow cytometry and inhibition of T cell responses to IL-21.

Each lot is tested by flow cytometry using freshly harvested mouse splenocytes. For PE-Texas Red conjugates: excitation 488 nm/peak emission 615 nm.

Use dim light during handling, incubation with cells, and prior to analysis. It is recommended that cells be analyzed within 18 hours of staining. If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

HFE inhibits type I IFNs signaling by targeting the SQSTM1-mediated MAVS autophagic degradation.

Autophagy

Liu J,Wu X,Wang H,Wei J,Wu Q,Wang X,Yan Y,Cui J,Min J,Wang F,Zhou J

MCD0817 was used in Flow cytometry/Cell sorting to identify 5 iron metabolism-associated genes specifically affected during RNA virus infection by a high-throughput assay and further found that HFE was a key negative regulator of RIG-I-like receptors (RLR)-mediated type I interferons (IFNs) signaling.

Sun Aug 01 00:00:00 EDT 2021

Combined OX40 Agonist and PD-1 Inhibitor Immunotherapy Improves the Efficacy of Vascular Targeted Photodynamic Therapy in a Urothelial Tumor Model.

Molecules (Basel, Switzerland)

Alvim RG,Georgala P,Nogueira L,Somma AJ,Nagar K,Thomas J,Alvim L,Riegel A,Hughes C,Chen J,Reis AB,Lebdai S,Scherz A,Zanganeh S,Gardner R,Kim K,Coleman JA

MCD0817 was used in Flow Cytometry to study the efficacy of the vascular targeted photodynamic therapy in combination with concurrent PD-1 inhibitor/OX40 agonist immunotherapy in a urothelial tumor-bearing model.

Sat Jun 19 00:00:00 EDT 2021

Blockade of the AHR restricts a Treg-macrophage suppressive axis induced by L-Kynurenine.

Nature communications

Campesato LF,Budhu S,Tchaicha J,Weng CH,Gigoux M,Cohen IJ,Redmond D,Mangarin L,Pourpe S,Liu C,Zappasodi R,Zamarin D,Cavanaugh J,Castro AC,Manfredi MG,McGovern K,Merghoub T,Wolchok JD

MCD0817 was used in Flow cytometry/Cell sorting to show that the AHR pathway is selectively active in IDO/TDO-overexpressing tumors and is associated with resistance to immune checkpoint inhibitors.

Tue Aug 11 00:00:00 EDT 2020

MicroRNA-155 Expression Is Enhanced by T-cell Receptor Stimulation Strength and Correlates with Improved Tumor Control in Melanoma.

Cancer immunology research

Martinez-Usatorre A,Sempere LF,Carmona SJ,Carretero-Iglesia L,Monnot G,Speiser DE,Rufer N,Donda A,Zehn D,Jandus C,Romero P

MCD0817 was used in Flow cytometry/Cell sorting to explore the regulation of miR-155 expression in CD8+ cells.

Sat Jun 01 00:00:00 EDT 2019

Non-conventional Inhibitory CD4⁺Foxp3^-PD-1^hi T Cells as a Biomarker of Immune Checkpoint Blockade Activity.

Cancer cell

Zappasodi R,Budhu S,Hellmann MD,Postow MA,Senbabaoglu Y,Manne S,Gasmi B,Liu C,Zhong H,Li Y,Huang AC,Hirschhorn-Cymerman D,Panageas KS,Wherry EJ,Merghoub T,Wolchok JD

MCD0817 was used in Flow cytometry/Cell sorting to provide evidence that mouse and human circulating and intra-tumor 4PD1hi inhibit T cell functions in a PD-1/PD-L1 dependent fashion and resemble follicular helper T cell-like cells.

Mon Jun 11 00:00:00 EDT 2018

11 Beta-hydroxysteroid dehydrogenase type 1 regulates synovitis, joint destruction, and systemic bone loss in chronic polyarthritis.

Journal of autoimmunity

Hardy RS,Fenton C,Croft AP,Naylor AJ,Begum R,Desanti G,Buckley CD,Lavery G,Cooper MS,Raza K

MCD0817 was used in Flow cytometry/Cell sorting to demonstrate a fundamental role for 11 beta-hydroxysteroid dehydrogenase type 1 in the suppression of synovitis, joint destruction, and systemic bone loss.

Wed Aug 01 00:00:00 EDT 2018

PD-1 Blockade Unleashes Effector Potential of Both High- and Low-Affinity Tumor-Infiltrating T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Martínez-Usatorre A,Donda A,Zehn D,Romero P

MCD0817 was used in Flow cytometry/Cell sorting to show that αPD-1 treatment enhances tumor control of high- and low-affinity ligand-expressing tumors, suggesting that combination of high-affinity peripheral priming by altered peptide ligands and checkpoint blockade may enable tumor control upon low-affinity Ag recognition in the tumor.

Sun Jul 15 00:00:00 EDT 2018

CART cells are prone to Fas- and DR5-mediated cell death.

Journal for immunotherapy of cancer

Tschumi BO,Dumauthioz N,Marti B,Zhang L,Lanitis E,Irving M,Schneider P,Mach JP,Coukos G,Romero P,Donda A

MCD0817 was used in Flow cytometry/Cell sorting to study the effects of in vivo blockade of Fas and DR5 to rescue chimeric antigen receptor T cells from apoptosis.

Fri Jul 13 00:00:00 EDT 2018

Hydrophobic CDR3 residues promote the development of self-reactive T cells.

Nature immunology

Stadinski BD,Shekhar K,Gómez-Touriño I,Jung J,Sasaki K,Sewell AK,Peakman M,Chakraborty AK,Huseby ES

MCD0817 was used in flow cytometry to study the promotion of the development of self-reactive T cells by hydrophobic CDR3 residues

Mon Aug 01 00:00:00 EDT 2016

Timing of CSF-1/CSF-1R signaling blockade is critical to improving responses to CTLA-4 based immunotherapy.

Oncoimmunology

Holmgaard RB,Brachfeld A,Gasmi B,Jones DR,Mattar M,Doman T,Murphy M,Schaer D,Wolchok JD,Merghoub T

MCD0817 was used in Flow cytometry/Cell sorting to show that both CSF-1 and its receptor (CSF-1R) are frequently expressed in tumors from cancer patients, and that this expression correlates with tumor-infiltration of MDSCs.

Fri Jul 01 00:00:00 EDT 2016

A spread-deficient cytomegalovirus for assessment of first-target cells in vaccination.

Journal of virology

Mohr CA,Arapovic J,Mühlbach H,Panzer M,Weyn A,Dölken L,Krmpotic A,Voehringer D,Ruzsics Z,Koszinowski U,Sacher T

MCD0817 was used in flow cytometry to characterize a spread-deficient murine cytomegalovirus as a novel approach for betaherpesvirus vaccination

Sun Aug 01 00:00:00 EDT 2010

Potential roles of a special CD8 alpha alpha+ cell population and CC chemokine thymus-expressed chemokine in ovulation related inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Zhou C,Wu J,Borillo J,Torres L,McMahon J,Lou YH

Published figure using CD8 alpha monoclonal antibody (Product # MCD0817) in Flow Cytometry

Thu Jan 01 00:00:00 EST 2009

Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.

Journal of immunology (Baltimore, Md. : 1950)

Schaeffer M,Han SJ,Chtanova T,van Dooren GG,Herzmark P,Chen Y,Roysam B,Striepen B,Robey EA

MCD0817 was used in flow cytometry to study interactions between CD8 T cells, Toxoplasma gondii, and APCs in the brain

Fri May 15 00:00:00 EDT 2009

Hematopoietic stem cell dose correlates with the speed of immune reconstitution after stem cell transplantation.

Blood

Chen BJ,Cui X,Sempowski GD,Domen J,Chao NJ

MCD0817 was used in flow cytometry to test if higher numbers of hematopoietic stem cells correlate with the speed of immune reconstitution in a congenic transplantation mouse model

Tue Jun 01 00:00:00 EDT 2004

Marked inhibition of transplant vascular sclerosis by in vivo-mobilized donor dendritic cells and anti-CD154 mAb.

Transplantation

Wang Z,Morelli AE,Hackstein H,Kaneko K,Thomson AW

MCD0817 was used in flow cytometry to determine the influence of in vivo-mobilized immature donor DC plus anti-CD40L mAb on vascular sclerosis in functional murine aortic allografts.

Fri Aug 15 00:00:00 EDT 2003

Immature and mature CD8alpha+ dendritic cells prolong the survival of vascularized heart allografts.

Journal of immunology (Baltimore, Md. : 1950)

O'Connell PJ,Li W,Wang Z,Specht SM,Logar AJ,Thomson AW

MCD0817 was used in flow cytometry to determine the role of CD8alpha-(myeloid) DCs in organ transplantation.

Tue Jan 01 00:00:00 EST 2002

Bioinformatics

Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

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Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

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UniProt ID: (Mouse) P01731, (Mouse) P10300

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Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

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Function(s)

protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 alpha Monoclonal Antibody (5H10), PE-Texas Red

CD8 alpha Antibody (MCD0817) in Flow

Product Details

MCD0817

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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